BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0046.0020.p (150 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogena... 135 1e-33 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 62 2e-11 gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogena... 62 2e-11 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 59 2e-10 >gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 135 bits (341), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 69/130 (53%), Positives = 90/130 (69%) Query: 4 ITLVTGASRGLGRNTALSIARHGGDVVVTYHSRSDEAQAVVAEIQSLGRKAVALQLDTGN 63 I L+TG SRGLG+NTAL +AR G V++TY A+A VAE+Q+LG A AL+LD + Sbjct: 10 IALITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQALGAVAAALRLDLAD 69 Query: 64 VAAFAAFAERLRAALRDTWSRDTFDHLVNNAGHGDYALIGDTTEAQFDGLVNVHFKGVYF 123 A+F AFA+ +R AL+ W + F +LVNN G G +A I +T+EA FD L VH KG +F Sbjct: 70 SASFPAFAQAVREALKTHWQAEHFHYLVNNGGMGMHASIEETSEAVFDALFKVHLKGPFF 129 Query: 124 LTQTLLPLIA 133 LTQ LLPL+A Sbjct: 130 LTQALLPLLA 139 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 62.0 bits (149), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 45/129 (34%), Positives = 64/129 (49%), Gaps = 9/129 (6%) Query: 6 LVTGASRGLGRNTALSIARHGGDVVVTYHSRSDEAQAVVAEIQSLGRKAVALQLDTGNVA 65 VTG+SRG+GR AL +AR G D+V+ H+R D A V AEI +LGR+ LQ D N Sbjct: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVLQFDVANRE 69 Query: 66 AFAAFAERLRAALRDTWSRDTFDHLVNNAG-HGDYALIGDTTEAQFDGLVNVHFKGVY-F 123 A + L D + +V NAG D A E +D ++ + G Y Sbjct: 70 QAA------QVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGE-DWDSVLRTNLDGFYNV 122 Query: 124 LTQTLLPLI 132 L ++P++ Sbjct: 123 LNPLVMPMV 131 >gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 61.6 bits (148), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 37/96 (38%), Positives = 57/96 (59%), Gaps = 7/96 (7%) Query: 4 ITLVTGASRGLGRNTALSIARHGGDVVVTYHSRSDEAQAVVAEIQSLGRKAVALQLDTGN 63 I LVTGA+R +GR+ AL++A+ G DV V Y+ +EA +V EI++LGR+A+A+Q + G+ Sbjct: 9 IALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIETLGRRALAVQAELGD 68 Query: 64 VAAFAAFAERLRAALRDTWSRDTFDHLVNNAGHGDY 99 ++R+ L +VNNA DY Sbjct: 69 -------ETQVRSLLPQAAPLGRISCVVNNASLFDY 97 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 58.5 bits (140), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 43/126 (34%), Positives = 65/126 (51%), Gaps = 6/126 (4%) Query: 1 MTSITLVTGASRGLGRNTALSIARHGGDVVVTYHSRSDEAQAVVAEIQSLGRKAVALQLD 60 ++ + V G SRG+G +A G V TY S D+AQA+V+ +Q+ G +A+A+Q D Sbjct: 16 VSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQAD 75 Query: 61 TGNVAAFAAFAERLRAALRDTWSRDTFDHLVNNAGHGDYALIGDTTEAQFDGLVNVHFKG 120 + + AA A RL A +T+ R D LVNNAG + D + V+ + Sbjct: 76 SADAAALQQ-AIRLAA---ETFGR--LDILVNNAGVLAMGPLDQFKLEDLDRTLAVNVRS 129 Query: 121 VYFLTQ 126 V+ TQ Sbjct: 130 VFVATQ 135 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 438,613 Number of extensions: 15393 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 6 Length of query: 150 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 73 Effective length of database: 1,255,275 Effective search space: 91635075 Effective search space used: 91635075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)