BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0044.0020.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314042|ref|YP_003778134.1| signal transduction protein con... 206 3e-54 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 192 4e-50 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 192 4e-50 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 181 6e-47 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 176 2e-45 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 206 bits (523), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 110/282 (39%), Positives = 169/282 (59%), Gaps = 18/282 (6%) Query: 11 FKIVNDSLGHLAGDAMIIQVAARLKNALRPDHPASSAEQGAGGVNPGYTLARMGGDEFTV 70 FK VND+LGH AGD ++ VA RL+ A+R T+AR+GGDEF + Sbjct: 561 FKFVNDTLGHKAGDTLLRTVAGRLRAAVRE----------------ADTVARLGGDEFIM 604 Query: 71 LLSDIDDVGEAVRIAERIQAAMALPFLIRDQEIYASASIGIVFGDIGHTLADEVLRDADL 130 +L + D++ R +RI A+A P + ++ + S+G+ T + +++ AD+ Sbjct: 605 ILPEQDELTLDTRSLQRIMDAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADI 664 Query: 131 AVGRAKTMGKSRYEVFDRSMRKGAVSRLAMETALRHALKNNEFVLHYQPIIELQGGTIVG 190 A+ R K G++ ++ + +M + A+ RL +E LR+AL + EF+LHYQP ++L G ++G Sbjct: 665 AMYRGKQTGRNNFQFYTPAMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIG 724 Query: 191 VEALVRWQRGPDELVYPGDFIDVAEDTGLILYIGMWVLREACRQMHQWHRQFPRAKPLTI 250 EAL+RWQ +V P FI +AE+TGLIL IG WVLR AC Q QW Q +++ Sbjct: 725 SEALIRWQHPQLGMVSPARFIGLAEETGLILPIGAWVLRAACTQCRQW--QLGGYADISV 782 Query: 251 SVNISPRQFVQPNLVDQIKQIVAETGIDPNTLRLEITESVTM 292 SVN+S QF+Q +LV I ++ +TG+ P +L LE+TES+ M Sbjct: 783 SVNLSVGQFLQKDLVSSIADVLEDTGLPPESLELELTESLMM 824 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 192 bits (487), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 110/287 (38%), Positives = 167/287 (58%), Gaps = 27/287 (9%) Query: 11 FKIVNDSLGHLAGDAMIIQVAARLKNALRPDHPASSAEQGAGGVNPGYTLARMGGDEFTV 70 FK +ND++GH GD ++ QVA RL +R + T+AR+GGDEF V Sbjct: 641 FKTLNDTIGHEKGDTLLKQVAQRLLANVREND----------------TVARVGGDEFVV 684 Query: 71 LLSDIDDVGEAVR-----IAERIQAAMALPFLIRDQEIYASASIG-IVFGDIGHTLA-DE 123 +L ++++ + I E+I A+ + + D E +ASIG VF +G + DE Sbjct: 685 VLEGLNELQQEAATQTRAIGEKILTALGSTYQLDDVEYRTTASIGATVF--LGRAASVDE 742 Query: 124 VLRDADLAVGRAKTMGKSRYEVFDRSMRKGAVSRLAMETALRHALKNNEFVLHYQPIIEL 183 +++ ADLA+ +AK G++ FD +M+ + R +E LR A+ N+ +LHYQP + + Sbjct: 743 LMKQADLAMYKAKETGRNALRFFDPAMQTAVLERATLEAGLRKAIDGNQLLLHYQPQV-V 801 Query: 184 QGGTIVGVEALVRWQRGPDELVYPGDFIDVAEDTGLILYIGMWVLREACRQMHQWHRQFP 243 +GG + G E LVRWQ +V PGDFI +AE+TGLIL +G WVL ACRQ+ W Q P Sbjct: 802 EGGRVTGAEVLVRWQHPQRGMVPPGDFIPLAEETGLILSLGNWVLETACRQLATWAAQ-P 860 Query: 244 RAKPLTISVNISPRQFVQPNLVDQIKQIVAETGIDPNTLRLEITESV 290 + L+I+VN+S Q +P+ V + + TG +P L+LE+TES+ Sbjct: 861 QMADLSIAVNVSAPQLREPDFVQTVLDTIERTGANPRRLKLELTESL 907 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 192 bits (487), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 119/291 (40%), Positives = 162/291 (55%), Gaps = 26/291 (8%) Query: 11 FKIVNDSLGHLAGDAMIIQVAARLKNALRPDHPASSAEQGAGGVNPGYTLARMGGDEFTV 70 FK +ND+ GH G+A++ ++A R +N+LR LAR+GGDEF V Sbjct: 712 FKNINDTFGHETGEAVLQEIAQRFRNSLRELD----------------ILARVGGDEFIV 755 Query: 71 LLSDIDDVGEAVRIAERIQAAMALPFLIRDQEIYASASIGIVF----GDIGHTLADEVLR 126 L+ D DD + IA+++ PF+I QE S SIGI GD TL ++ Sbjct: 756 LVDDFDDPVQLGEIAQKLLTEARKPFIIDGQEAMLSVSIGIATYPGDGDNAQTL----IK 811 Query: 127 DADLAVGRAKTMGKSRYEVFDRSMRKGAVSRLAMETALRHALKNNEFVLHYQPIIELQGG 186 +AD+A+ RAK GK Y F + V R+A+E LR A++ EFV+HYQP IEL G Sbjct: 812 NADIAMYRAKHHGKDDYRFFSDEINTNTVERIALEAELRRAIERAEFVVHYQPKIELAEG 871 Query: 187 TIVGVEALVRWQRGPDELVYPGDFIDVAEDTGLILYIGMWVLREACRQMHQWHRQFPRAK 246 IVG EALVRWQ L+ PG+FI +AE+T LI IG+ VL ACR + +Q + Sbjct: 872 RIVGAEALVRWQHPLRGLLPPGEFITLAEETRLIDQIGLLVLDVACRDIGLMRQQ--QMT 929 Query: 247 PLTISVNISPRQFVQPNLVDQIKQIVAETGIDPNTLRLEITESVTMXARWR 297 IS+N++ QF L+D IK++V G P+ L EITES+ M R R Sbjct: 930 CGRISINLTGSQFGDERLLDDIKEVVDRHGTPPSCLEFEITESMVMHNRER 980 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 181 bits (459), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 112/285 (39%), Positives = 161/285 (56%), Gaps = 24/285 (8%) Query: 11 FKIVNDSLGHLAGDAMIIQVAARLKNALRPDHPASSAEQGAGGVNPGYTLARMGGDEFTV 70 FK +NDSLGH GD ++ VA RL+ LR +AR GGDEF + Sbjct: 308 FKFINDSLGHQVGDELLKTVAQRLQACLRE----------------ADMVARQGGDEFVL 351 Query: 71 LLSDIDDVGEA--VRIAERIQAAMALPFLIRDQEIYASASIGIVFGDIGHTLADEVLRDA 128 +L + + GEA + +RI AA+A P+ D+E +ASIG+ + +L+ A Sbjct: 352 VLQNQTE-GEAGIAEVMQRILAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHA 410 Query: 129 DLAVGRAKTMGKSRYEVFDRSMRKGAVSRLAMETALRHALKNNEFVLHYQPIIELQGGTI 188 D A+ RAK G++ ++ F M +RL M LR AL +EF L+YQP + LQ G + Sbjct: 411 DSAMYRAKEQGRNNFQFFAPWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRL 470 Query: 189 VGVEALVRWQRGPDELVYPGDFIDVAEDTGLILYIGMWVLREACRQMHQWHRQ-FPRAKP 247 +G EAL+RW+ +V P FI AE+ GLI+ IG WVLR ACRQ W + P P Sbjct: 471 IGAEALIRWESPQQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLP---P 527 Query: 248 LTISVNISPRQFVQPNLVDQIKQIVAETGIDPNTLRLEITESVTM 292 + ++VN+SPRQ Q +L D + ++A++G+ + L LEITE+V M Sbjct: 528 IPVAVNLSPRQLNQ-SLPDFVAGVLAQSGLAASWLELEITENVVM 571 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 176 bits (446), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 111/284 (39%), Positives = 162/284 (57%), Gaps = 23/284 (8%) Query: 11 FKIVNDSLGHLAGDAMIIQVAARLKNALRPDHPASSAEQGAGGVNPGYTLARMGGDEFTV 70 FK +NDSLGH GD ++++VA RL ++ R + T+AR+GGDEF V Sbjct: 308 FKAINDSLGHHVGDRLLVEVAQRLTSSSRAND----------------TVARLGGDEF-V 350 Query: 71 LLSDIDDVGEAVRIAERIQAAMALPFLIRDQEIYASASIGI-VFGDIGHTLADEVLRDAD 129 +L D++ +A+ +AE++ A+ PF + E+ SAS+GI ++ + G T D V+ +AD Sbjct: 351 ILVDLNTPEDAMGVAEKLVEAVNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVI-NAD 409 Query: 130 LAVGRAKTMGKSRYEVFDRSMRKGAVSRLAMETALRHALKNNEFVLHYQPIIELQGGTIV 189 A+ K G++ Y F+ SM A +L LR AL+ +F L YQP L G ++ Sbjct: 410 AAMYHTKRSGRNGYHFFEPSMNANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVL 469 Query: 190 GVEALVRWQRGPDELVYPGDFIDVAEDTGLILYIGMWVLREACRQMHQWHRQFPRAK-PL 248 G EAL+RW+ LV P FI +AE +GLIL IG WVL EACRQM +W F Sbjct: 470 GAEALLRWEHPVHGLVAPDQFIGLAERSGLILPIGAWVLDEACRQMREW---FALGYVDW 526 Query: 249 TISVNISPRQFVQPNLVDQIKQIVAETGIDPNTLRLEITESVTM 292 T+SVN+S QF P+L++ ++ + + P L LE+TES M Sbjct: 527 TMSVNLSALQFADPHLLEVVRTTLERHQLPPPCLTLEVTESTAM 570 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 979,280 Number of extensions: 42273 Number of successful extensions: 231 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 24 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)