BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0028.p (234 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 158 4e-40 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 148 3e-37 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 135 2e-33 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 84 7e-18 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 75 3e-15 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 158 bits (399), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 81/207 (39%), Positives = 116/207 (56%), Gaps = 1/207 (0%) Query: 14 AVERFVAERLLPNETLVEEQRRVPDDILGEMAALGLFGLGIPERWGGLGLGMEDEVAVLI 73 A+ F ERLLP + P + L E+A LG G+ +PE+WGG G+ V L Sbjct: 13 AMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVLALE 72 Query: 74 ALCRASLSFRSAIGINNGVGSHGLLSYGSDAQRERYLARMAAGEMIAAFCLTEPDCGSDA 133 + + + + + N + YGS Q+E++L +A GEM+ FCLTEP GSDA Sbjct: 73 EIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDA 132 Query: 134 AALRTSARRDGDQWVLNGVKRYITNAPSADVFTVIARTDAGSRDHRGLSAFIVERGAPGL 193 AA+RT A RDGD ++LNG K++IT+ A V V A TD S +G+S F+V PG Sbjct: 133 AAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDR-SAGKKGISCFLVPCDTPGF 191 Query: 194 SIGNTDRKMGLHGSHTADVIFSDCRIP 220 +G ++ KMG H S T ++ +CR+P Sbjct: 192 VVGRSEEKMGQHASDTVQIMLDNCRVP 218 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 148 bits (374), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 73/209 (34%), Positives = 123/209 (58%), Gaps = 5/209 (2%) Query: 14 AVERFVAERLLPNETLVEEQRRVPDDILGEMAALGLFGLGIPERWGGLGLGMEDEVAVLI 73 AV F + P ++ + P D+ ++ LG+ G+ + E +GG GLG + + Sbjct: 21 AVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGYLAHIIAME 80 Query: 74 ALCRASLSFRSAIGINNGVGSHGLLSYGSDAQRERYLARMAAGEMIAAFCLTEPDCGSDA 133 + RAS S + G ++ + + + G++ Q+ +YL R+ +G+ I A ++EP+ GSD Sbjct: 81 EISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFIGALAMSEPNAGSDV 140 Query: 134 AALRTSARRDGDQWVLNGVKRYITNAPSADVFTVIARTD--AGSRDHRGLSAFIVERGAP 191 +++ A + GD++VLNG K +ITN P ADV V A+TD AG+ RG++AF+VE+G Sbjct: 141 VSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGA---RGMTAFLVEKGYK 197 Query: 192 GLSIGNTDRKMGLHGSHTADVIFSDCRIP 220 G S+ K+G+ GSHT +++F DC +P Sbjct: 198 GFSVAQKLDKLGMRGSHTGELVFQDCEVP 226 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 135 bits (341), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 79/210 (37%), Positives = 116/210 (55%), Gaps = 3/210 (1%) Query: 11 FVAAVERFVAERLLPNETLVEEQRRVPDDILGEMAALGLFGLGIPERWGGLGLGMEDEVA 70 F + F A + P +E+ P D++ + LG GL PE GGLGL D Sbjct: 12 FQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGLSRLDATV 71 Query: 71 VLIALCRASLSFRSAIGINNGVGSHGLLSYGSDAQRERYLARMAAGEMIAAFCLTEPDCG 130 V L RA S + + I+N V S + S+G + + ++ ++AAG I ++CLTEP G Sbjct: 72 VFEELARACTSTAAYLTIHNMV-SWMIASWGQPSLKAQWCEQLAAGRKIGSYCLTEPGSG 130 Query: 131 SDAAALRTSARRDGDQWVLNGVKRYITNAPSADVFTVIARTDAGSRDHRGLSAFIVERGA 190 SDAA+L+T+AR + +VLNG K +I+ A S DV V+ART G RG+SA +V A Sbjct: 131 SDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMART--GGEGARGVSAIVVPGNA 188 Query: 191 PGLSIGNTDRKMGLHGSHTADVIFSDCRIP 220 PG+S G + KMG + T + F + ++P Sbjct: 189 PGVSYGKKESKMGWNSQPTRTISFDNVKVP 218 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 84.3 bits (207), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 65/226 (28%), Positives = 103/226 (45%), Gaps = 18/226 (7%) Query: 9 KQFVAAVERFVAERLLPNETLVEEQRRV--PDDILGEMAALGLFGLGIPERWGGLGLGME 66 + F A V R V + P LV+ R MA G +PE +GG L Sbjct: 19 RAFAADVLRKVGPTITP---LVKPDERFYATRPFYQAMADAGFIKALLPESFGGTALSTL 75 Query: 67 DEVAVLIALCRASLSFRSAIGINNGVGSHGLLSYGSDAQRERYLARMAA-GEMIAAFCLT 125 D L ++ SA+ + G+G H ++ +G+ Q++R+L G +AA T Sbjct: 76 DFALAAEELTAVDVNVPSAL-LGTGLGLHAVVHHGTPEQKQRFLPDFIEDGSRLAALAFT 134 Query: 126 EPDCGSD------AAALRTSARRDGDQWVLNGVKRYITNAPSAD-----VFTVIARTDAG 174 E G++ + ++T AR +GD+WV+NG K Y TN D +++VI RTD Sbjct: 135 EVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTGWDGKGAHLYSVICRTDPA 194 Query: 175 SRDHRGLSAFIVERGAPGLSIGNTDRKMGLHGSHTADVIFSDCRIP 220 + L+ +V APG+ I MG + + V F++ R+P Sbjct: 195 LPPQQSLAVIMVPGNAPGIEIVGLLDTMGHRATISPRVHFNNVRVP 240 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 75.5 bits (184), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 57/196 (29%), Positives = 105/196 (53%), Gaps = 8/196 (4%) Query: 30 VEEQRRVPDDILGEMAALGLFGLGIPERWGGLGLGMEDEVAVLIALCRASLSFRSAIGIN 89 ++E R P+ + + G IP+ +GG GLG+ + ++ + R + + G Sbjct: 32 IDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLGLTEASVIMEEINRTGGNSGACHGQM 91 Query: 90 NGVGSHGLLSYGSDAQRERYLARMAAGEM-IAAFCLTEPDCGSDAAALRTSARRDGDQWV 148 +G+ LL +GS Q+ERYL R+A+GE+ + + +TEP G+D ++T A + GD++V Sbjct: 92 YNMGT--LLRHGSPEQKERYLPRIASGELRLQSMAVTEPTTGTDTTRIKTVAVKKGDRYV 149 Query: 149 LNGVKRYITNAPSADVFTVIART---DAGSRDHRGLSAFIVE-RGAPGLSIGNTDRKMGL 204 +NG K +I+ +D+ ++ART + SR G+S F+V+ + A G + + + Sbjct: 150 INGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMSIFLVDLKEAIGHGM-EVRPILNM 208 Query: 205 HGSHTADVIFSDCRIP 220 T ++ F + IP Sbjct: 209 VNHETNELFFDNLEIP 224 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 731,982 Number of extensions: 32615 Number of successful extensions: 111 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 101 Number of HSP's successfully gapped: 9 Length of query: 234 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 152 Effective length of database: 1,231,600 Effective search space: 187203200 Effective search space used: 187203200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)