BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0027.p (135 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 82 1e-17 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 76 8e-16 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 75 2e-15 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 64 5e-12 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 57 6e-10 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 82.4 bits (202), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 48/130 (36%), Positives = 67/130 (51%), Gaps = 24/130 (18%) Query: 4 KVVNISSVSGVMGNAGQANYSAAKSAINGLTKALAKEWGRYKVNVNSVAFGLIKTRLTEA 63 ++V ++SVSG++GN GQ NYSAAK+ + G TKALA E + + VN VA GLI T +TE Sbjct: 138 RIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVNCVAPGLIDTDMTED 197 Query: 64 TAGADSAGATINIAGNEIKVGVNQQLMQNLETMIPMGRAGTPAEAAGAVYLFCIPESNYI 123 + + +IP RAG P E A AV P++ YI Sbjct: 198 AP------------------------IDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYI 233 Query: 124 SGQIVICGGG 133 + Q++ GG Sbjct: 234 TRQVISVNGG 243 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 76.3 bits (186), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 46/130 (35%), Positives = 67/130 (51%), Gaps = 21/130 (16%) Query: 4 KVVNISSVSGVMGNAGQANYSAAKSAINGLTKALAKEWGRYKVNVNSVAFGLIKTRLTEA 63 +++NI+SV G GN GQ NY+AAK+ + G+++ALA+E G + VN VA G I T +T A Sbjct: 139 RIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAPGFIDTDMTRA 198 Query: 64 TAGADSAGATINIAGNEIKVGVNQQLMQNLETMIPMGRAGTPAEAAGAVYLFCIPESNYI 123 +N+Q + IP+GR G E A AV P++ YI Sbjct: 199 ---------------------LNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYI 237 Query: 124 SGQIVICGGG 133 +G + GG Sbjct: 238 TGTTLNVNGG 247 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 75.1 bits (183), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 44/130 (33%), Positives = 68/130 (52%), Gaps = 20/130 (15%) Query: 4 KVVNISSVSGVMGNAGQANYSAAKSAINGLTKALAKEWGRYKVNVNSVAFGLIKTRLTEA 63 ++VNISS+SG+ AG+ Y +K+A+ GLT+ +A E +Y + VNSVA G + T +T+A Sbjct: 138 RIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPGPVDTPMTQA 197 Query: 64 TAGADSAGATINIAGNEIKVGVNQQLMQNLETMIPMGRAGTPAEAAGAVYLFCIPESNYI 123 ++Q ++ ++PM R GT E A AV ES YI Sbjct: 198 MH--------------------SEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYI 237 Query: 124 SGQIVICGGG 133 +G ++ GG Sbjct: 238 TGHVIPVDGG 247 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 63.5 bits (153), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 43/132 (32%), Positives = 62/132 (46%), Gaps = 21/132 (15%) Query: 2 FRKVVNISSVSGVMGNAGQANYSAAKSAINGLTKALAKEWGRYKVNVNSVAFGLIKTRLT 61 F +++NISSV+G G GQ NYS AK+ I+G T ALA+E V VN+V+ G I T + Sbjct: 132 FGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGYIGTDMV 191 Query: 62 EATAGADSAGATINIAGNEIKVGVNQQLMQNLETMIPMGRAGTPAEAAGAVYLFCIPESN 121 A + ++++ + + IP+ R G P E A V E Sbjct: 192 RA---------------------IRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGG 230 Query: 122 YISGQIVICGGG 133 Y +G GG Sbjct: 231 YATGSDFSLNGG 242 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 56.6 bits (135), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 41/131 (31%), Positives = 64/131 (48%), Gaps = 21/131 (16%) Query: 5 VVNISSVSGVMGNAGQ-ANYSAAKSAINGLTKALAKEWGRYKVNVNSVAFGLIKTRLTEA 63 +VN+SS++ +G G+ +Y+A+K AI+ T LAKE + VN+V GLI T Sbjct: 141 IVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEGIRVNAVRPGLIYT----- 195 Query: 64 TAGADSAGATINIAGNEIKVGVNQQLMQNLETMIPMGRAGTPAEAAGAVYLFCIPESNYI 123 I+ +G E + L+ +PM R G+ AE A A+ E++Y Sbjct: 196 ---------DIHASGGE------AGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYA 240 Query: 124 SGQIVICGGGR 134 +G + GGR Sbjct: 241 TGTFIDVSGGR 251 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 393,054 Number of extensions: 14417 Number of successful extensions: 111 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 104 Number of HSP's successfully gapped: 13 Length of query: 135 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 59 Effective length of database: 1,260,010 Effective search space: 74340590 Effective search space used: 74340590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)