BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0026.p (321 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 135 4e-33 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 113 2e-26 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 97 2e-21 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 60 2e-10 gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbasp... 56 3e-09 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 135 bits (341), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 105/378 (27%), Positives = 163/378 (43%), Gaps = 74/378 (19%) Query: 9 LDPDASKWRDIAHRLAHDQLAPRAAAIDRDQRYPHENVEALHESGIASMFIPKDYGGGGA 68 L P+ RD A ++L P AA DR+ +P E ++ L E G M +P+ +GG G Sbjct: 3 LSPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGM 62 Query: 69 SLTAFCAVIEELAQACASTSGIVATLQLGAMPLL-RAGTTGQKDTYIRAMVAGRKSISFA 127 + +EE+A +TS IV+ A + + G+ QK+ +++ + G F Sbjct: 63 DYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFC 122 Query: 128 LSEREAGSDPSNMQTVATAENRGWRLRGEKRWIGGGGECAYYVVFAQTEPGAGRRGIAAF 187 L+E GSD + ++T A + + L G K++I G +VFA T+ AG++GI+ F Sbjct: 123 LTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCF 182 Query: 188 MVAADAEGVRDDARXTTFGQR------------------LLDHQGIGFKLADVATRLSAG 229 +V D G GQ LL +G G+++A + L AG Sbjct: 183 LVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIA--LSNLEAG 240 Query: 230 RM-----------LAYETARSF-------------------------------------- 240 R+ A+E A S+ Sbjct: 241 RIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRA 300 Query: 241 ----DTGQDVALIGAQAKLFCSEAAHDAVDIGVQVFGGEGFVKPSLVERLYRDQRATEIY 296 D G+ + AK+F SEAA +Q+ GG G+ VER+YRD R +IY Sbjct: 301 AQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIY 360 Query: 297 EGTSEIQRLVLARAIQAE 314 EG ++IQRLV+ RAI ++ Sbjct: 361 EGANDIQRLVIGRAIASD 378 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 113 bits (282), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 100/373 (26%), Positives = 160/373 (42%), Gaps = 71/373 (19%) Query: 9 LDPDASKWRDIAHRLAHDQLAPRAAAIDRDQRYPHENVEALHESGIASMFIPKDYGGGGA 68 L+ D ++ A A ++AP AA D + +P + + E G ++ P+ GG G Sbjct: 5 LNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGL 64 Query: 69 SLTAFCAVIEELAQACASTSGIVATLQLGAMPLLRAGTTGQKDTYIRAMVAGRKSISFAL 128 S V EELA+AC ST+ + + + + G K + + AGRK S+ L Sbjct: 65 SRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGRKIGSYCL 124 Query: 129 SEREAGSDPSNMQTVATAENRGWRLRGEKRWIGGGGECAYYVVFAQTEPGAGRRGIAAFM 188 +E +GSD ++++T A E+ + L G K +I G G VV A+T G G RG++A + Sbjct: 125 TEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMART-GGEGARGVSAIV 183 Query: 189 VAADAEGVRDDARXTTFG------------------QRLLDHQGIGFKLA---------D 221 V +A GV + + G LL +G GF A + Sbjct: 184 VPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGRIN 243 Query: 222 VAT-------------------RLSAGRMLAYETARSFDTG-QDVALIGA---------- 251 +AT R GR +A A F L+ A Sbjct: 244 IATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLAATK 303 Query: 252 ------QAKLFCSEAAHDAVDIG-------VQVFGGEGFVKPSLVERLYRDQRATEIYEG 298 +A +C+ A A D+G +Q+ GG G+++ +ER +RD R +I EG Sbjct: 304 LDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQILEG 363 Query: 299 TSEIQRLVLARAI 311 T+EI R++++R + Sbjct: 364 TNEIMRVIISRKL 376 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 96.7 bits (239), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 62/198 (31%), Positives = 99/198 (50%), Gaps = 1/198 (0%) Query: 12 DASKWRDIAHRLAHDQLAPRAAAIDRDQRYPHENVEALHESGIASMFIPKDYGGGGASLT 71 D + R+ AH ++APRAA IDR ++P + + L + G+ + + ++YGG G Sbjct: 14 DIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGYL 73 Query: 72 AFCAVIEELAQACASTS-GIVATLQLGAMPLLRAGTTGQKDTYIRAMVAGRKSISFALSE 130 A +EE+++A AS A L + R G QK Y+ +++G + A+SE Sbjct: 74 AHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFIGALAMSE 133 Query: 131 REAGSDPSNMQTVATAENRGWRLRGEKRWIGGGGECAYYVVFAQTEPGAGRRGIAAFMVA 190 AGSD +M+ A + + L G K WI G + VV+A+T+ AG RG+ AF+V Sbjct: 134 PNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGARGMTAFLVE 193 Query: 191 ADAEGVRDDARXTTFGQR 208 +G + G R Sbjct: 194 KGYKGFSVAQKLDKLGMR 211 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 60.5 bits (145), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 46/160 (28%), Positives = 80/160 (50%), Gaps = 6/160 (3%) Query: 35 IDRDQRYPHENVEALHESGIASMFIPKDYGGGGASLTAFCAVIEELAQACASTSGIVATL 94 ID + YP + V+AL ++G + IP++YGG G LT ++EE+ + SG Sbjct: 32 IDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLGLTEASVIMEEINRT-GGNSGACHGQ 90 Query: 95 QLGAMPLLRAGTTGQKDTYIRAMVAGRKSI-SFALSEREAGSDPSNMQTVATAENRGWRL 153 LLR G+ QK+ Y+ + +G + S A++E G+D + ++TVA + + + Sbjct: 91 MYNMGTLLRHGSPEQKERYLPRIASGELRLQSMAVTEPTTGTDTTRIKTVAVKKGDRYVI 150 Query: 154 RGEKRWIGGGGECAYYVVFAQTEP----GAGRRGIAAFMV 189 G+K WI ++ A+T P G++ F+V Sbjct: 151 NGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMSIFLV 190 >gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 56.2 bits (134), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 39/138 (28%), Positives = 64/138 (46%), Gaps = 6/138 (4%) Query: 183 GIAAFMVAADAEGVRDDARXTTFGQRLLDHQGIGFKLADVATRLSAGRMLAYETARSFDT 242 G AA M A + + + FG+RL + + LAD+A A ML A +FD Sbjct: 307 GSAALMRQALVQAIHHARHRSAFGRRLAEQPLMRAVLADLALESEAATMLMLRVAHAFDA 366 Query: 243 GQDVA------LIGAQAKLFCSEAAHDAVDIGVQVFGGEGFVKPSLVERLYRDQRATEIY 296 D +I AK + + + ++V+GG G+V+ + + RLYR+ I+ Sbjct: 367 PDDPLQRAWKRIITPAAKFWICKRTLEFTGECMEVWGGNGYVETAPMARLYREAPVNSIW 426 Query: 297 EGTSEIQRLVLARAIQAE 314 EG+ + L + RA+ E Sbjct: 427 EGSGNVMCLDVLRAMARE 444 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,037,003 Number of extensions: 44768 Number of successful extensions: 167 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 7 Length of query: 321 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 236 Effective length of database: 1,217,395 Effective search space: 287305220 Effective search space used: 287305220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)