BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0020.p (342 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312970|ref|YP_003777062.1| nitrate/sulfonate/bicarbonate A... 63 3e-11 >gi|300312970|ref|YP_003777062.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 345 Score = 63.2 bits (152), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 58/187 (31%), Positives = 92/187 (49%), Gaps = 16/187 (8%) Query: 6 AVNAQAQAREKVTIAIGTDVLDASQVNNTSLPIYTK----CWENAGLDVTLQP-TNSTTA 60 + AQA + KV+IA+G L LP+ +++ GL V + A Sbjct: 25 SAGAQALEKPKVSIAVGGKNL------FYYLPLTIAEQLGYFKDEGLQVEISDFAGGAKA 78 Query: 61 MQAVLTGQADMVNMGPAAAIIARTKGAPIKAVYLNMRHNFQFPVVLDSSPV---KTLADF 117 +QA++ G AD+V+ I + K PI A L R Q + +++ + KT+AD Sbjct: 79 LQAMVGGSADVVSGAYEHTINMQAKNQPIVAFVLQGRAP-QIVLAVNNKTMPNYKTVADL 137 Query: 118 KGKTIGVLSYGSQMVQIFKGMLTEAGLNPDKDVTFVETGSGAQAVTALSKGRVDIWGTWD 177 KGK IGV + GS + +L +AGL P DV+F+ G+ A A++A+ G++D D Sbjct: 138 KGKKIGVTAPGSSTSVMASYVLAKAGLKPS-DVSFIGVGAAAGAISAVRSGQIDAIANLD 196 Query: 178 SQIATAE 184 I+ E Sbjct: 197 PVISMLE 203 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 971,177 Number of extensions: 39197 Number of successful extensions: 142 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 2 Length of query: 342 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 256 Effective length of database: 1,212,660 Effective search space: 310440960 Effective search space used: 310440960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)