BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0014.p (80 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310385|ref|YP_003774477.1| metal-dependent hydrolase [Herb... 83 5e-18 gi|300313781|ref|YP_003777873.1| metal-dependent hydrolase [Herb... 70 3e-14 >gi|300310385|ref|YP_003774477.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] Length = 289 Score = 82.8 bits (203), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 37/49 (75%), Positives = 42/49 (85%) Query: 1 VALLQERSYVYDVLVFERQLADTQAFCARHDKHWLVLDHLGKPALREFG 49 VA LQ + VYDVLV+ RQ+A+ QAFCARHD+HWLVLDHLGKPAL EFG Sbjct: 128 VAWLQRQGMVYDVLVYARQMAEVQAFCARHDRHWLVLDHLGKPALTEFG 176 >gi|300313781|ref|YP_003777873.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] Length = 299 Score = 70.5 bits (171), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 33/52 (63%), Positives = 38/52 (73%) Query: 1 VALLQERSYVYDVLVFERQLADTQAFCARHDKHWLVLDHLGKPALREFGNRR 52 V LQERSY DVL ER L + FCARHD+HW+VL+HLGKPAL+EF R Sbjct: 137 VRWLQERSYTLDVLAHERHLPQLRQFCARHDQHWVVLNHLGKPALKEFRKGR 188 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 255,452 Number of extensions: 9043 Number of successful extensions: 17 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 17 Number of HSP's successfully gapped: 2 Length of query: 80 Length of database: 1,619,870 Length adjustment: 51 Effective length of query: 29 Effective length of database: 1,378,385 Effective search space: 39973165 Effective search space used: 39973165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 123 (52.0 bits)