BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0013.p (100 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 90 3e-20 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 72 9e-15 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 90.1 bits (222), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 47/98 (47%), Positives = 59/98 (60%), Gaps = 2/98 (2%) Query: 1 VVKALWDSWEDGAMIGDKAGGRFIDTAKVQPIAHRGTHFSVHGPLNLPRTPQGHPVLVQA 60 V LW SW+ A+ + R+ D KV I H+G HFSV GPLN+P +PQGHPV++QA Sbjct: 164 VTHKLWHSWQPDALRLQQ--DRYADADKVAAIDHQGAHFSVRGPLNIPASPQGHPVIIQA 221 Query: 61 GGSADGRDLAARHAEAVFTASQSFEESLAYGRELKARA 98 G S G+D AAR AE VF A+ R+LK RA Sbjct: 222 GSSDRGQDFAARWAEVVFNIQPDLPRLQAFYRDLKNRA 259 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 72.0 bits (175), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 41/84 (48%), Positives = 51/84 (60%), Gaps = 1/84 (1%) Query: 1 VVKALWD-SWEDGAMIGDKAGGRFIDTAKVQPIAHRGTHFSVHGPLNLPRTPQGHPVLVQ 59 VV LW+ SWEDGA++ D+A + D AKV IAH G ++ V G + Q PVL Q Sbjct: 168 VVYKLWEGSWEDGAVLRDRARRVYADPAKVHKIAHHGDYYHVEGIHLSEPSRQRTPVLYQ 227 Query: 60 AGGSADGRDLAARHAEAVFTASQS 83 AG S G+ ARHAE VF +SQS Sbjct: 228 AGSSGRGQAFGARHAECVFISSQS 251 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 372,633 Number of extensions: 14972 Number of successful extensions: 50 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 50 Number of HSP's successfully gapped: 2 Length of query: 100 Length of database: 1,619,870 Length adjustment: 69 Effective length of query: 31 Effective length of database: 1,293,155 Effective search space: 40087805 Effective search space used: 40087805 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)