BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0005.p (153 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogena... 284 2e-78 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 78 3e-16 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 77 6e-16 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 73 1e-14 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 68 3e-13 >gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 284 bits (727), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 129/147 (87%), Positives = 142/147 (96%) Query: 7 KNLIHYYVMAHYCVPHLKATRGAIVNVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAAL 66 +NLIHYYVMAHYCVPHLKATRGAI+NVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAAL Sbjct: 112 RNLIHYYVMAHYCVPHLKATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAAL 171 Query: 67 ANDGVRVNALIPAEVMTPLYEKWISSFDNPQEKIASIASKIPFGKRFTTSEEMADTAVFL 126 +DGVRVNALIPAEVMTPLYEKWI++FD+PQEK+ +I SKIP GKRFTTSEEMAD AVFL Sbjct: 172 RDDGVRVNALIPAEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFL 231 Query: 127 LSDRAAHTTGQWVFVDGGYTHLDRALS 153 LS+R++HTTGQW+FVDGGYTHLDRAL+ Sbjct: 232 LSERSSHTTGQWIFVDGGYTHLDRALT 258 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 78.2 bits (191), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 49/123 (39%), Positives = 66/123 (53%), Gaps = 5/123 (4%) Query: 23 LKATRGAIVNVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALANDGVRVNALIPAEVM 82 L+ +RG IVN +S + G G GY ASKG LT+ A A A DG+RVNA+ P + Sbjct: 121 LRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIA 180 Query: 83 TPLYEKWISSFDNPQEKIASIASKIPFGKRFTTSEEMADTAVFLLSDRAAHTTGQWVFVD 142 TPL + D+P + I ++ P G R+ E++ FL S A+ TG V VD Sbjct: 181 TPLTQAL---QDDPV-RAGPILARTPLG-RWGQPEDVGQVVAFLCSPAASFMTGAIVPVD 235 Query: 143 GGY 145 GGY Sbjct: 236 GGY 238 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 77.0 bits (188), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 50/145 (34%), Positives = 77/145 (53%), Gaps = 9/145 (6%) Query: 1 MAHYCXKNLIHYYVMAHYCVPHLKATRGA-IVNVSSKTAVTGQGNTSGYCASKGAQLSLT 59 + H N+ + M +P L TRGA I+N +S TA G + Y A+KGA ++ T Sbjct: 228 LEHTFRTNIFSMFYMVKEAMPFL--TRGARIINTTSVTAYRGSKHLVDYAATKGAIVAFT 285 Query: 60 REWAAALANDGVRVNALIPAEVMTPLYEKWISSFDNPQEKIASIASKIPFGKRFTTSEEM 119 R + LA+ G+ VNA+ P + TPL S+ E++AS +P G R +E+ Sbjct: 286 RSLSQQLASAGIHVNAVAPGPIWTPLIPASFSA-----EEVASFGKNVPLG-RPGQPDEV 339 Query: 120 ADTAVFLLSDRAAHTTGQWVFVDGG 144 A VFL ++ A++ TGQ + +GG Sbjct: 340 APAYVFLATEGASYMTGQVLHPNGG 364 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 72.8 bits (177), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 44/138 (31%), Positives = 75/138 (54%), Gaps = 3/138 (2%) Query: 8 NLIHYYVMAHYCVPHLKATRG-AIVNVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAAL 66 N+ ++ A VP+ + G A +N++S + + + Y SKGA ++ ++ AA L Sbjct: 114 NVKSIFLSAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAEL 173 Query: 67 ANDGVRVNALIPAEVMTPLYEKWISSFDNPQEKIASIASKIPFGKRFTTSEEMADTAVFL 126 D +RVN + P T L +++ D P+ + +A+ IP G RF+T E++A+ ++L Sbjct: 174 GPDKIRVNCVNPVISATGLLSEFMGVPDTPENRKKFVAT-IPMG-RFSTPEDIANACLYL 231 Query: 127 LSDRAAHTTGQWVFVDGG 144 SD A TG + VDGG Sbjct: 232 GSDEAEFVTGVCIEVDGG 249 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 68.2 bits (165), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 41/120 (34%), Positives = 60/120 (50%), Gaps = 2/120 (1%) Query: 29 AIVNVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALANDGVRVNALIPAEVMTPLYEK 88 +++ SS T+V G N S Y ASK A + R WA L + +RVN + P + TP + Sbjct: 132 SVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVNVVSPGPIRTPGLGE 191 Query: 89 WISSFDNPQEKIASIASKIPFGKRFTTSEEMADTAVFLLSDRAAHTTGQWVFVDGGYTHL 148 + D Q +AS++P G R +E+ FL SD A+ G +FVDGG + Sbjct: 192 LVPD-DARQGLFDYLASQVPLG-RLGEPQEIGKAVAFLASDAASFVNGIELFVDGGMAQV 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.130 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 467,218 Number of extensions: 16446 Number of successful extensions: 123 Number of sequences better than 1.0e-08: 26 Number of HSP's gapped: 115 Number of HSP's successfully gapped: 26 Length of query: 153 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 76 Effective length of database: 1,255,275 Effective search space: 95400900 Effective search space used: 95400900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)