BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0003.p (129 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogena... 90 5e-20 gi|300310381|ref|YP_003774473.1| hypothetical protein Hsero_1048... 71 3e-14 >gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 90.1 bits (222), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 43/78 (55%), Positives = 57/78 (73%) Query: 1 VFGKSRANDAFREQLLKLQPQARFFLLDLMDEEKCRQAVAETVAAYGRLDGLVNNAGVND 60 VF +S F E+L +QP+A F L+L D+ +CR+AVA T+ +GRLDGLVNNAGVND Sbjct: 36 VFARSAPESQFWERLTAVQPRAALFQLELQDDARCREAVAGTIERFGRLDGLVNNAGVND 95 Query: 61 NVGLDAGRAAFMQSLEKK 78 +VGL+AGR F+ SLE+ Sbjct: 96 SVGLEAGRDEFVASLERN 113 >gi|300310381|ref|YP_003774473.1| hypothetical protein Hsero_1048 [Herbaspirillum seropedicae SmR1] Length = 120 Score = 71.2 bits (173), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 29/38 (76%), Positives = 33/38 (86%) Query: 92 PKVQEWETLMWQFQAPTPWTPQGEKWIPMAKIFDLTEQ 129 PKV+EWE LMW+FQAPTPWTP G+KWIPM +IFDL Q Sbjct: 75 PKVREWEELMWRFQAPTPWTPTGQKWIPMTRIFDLAGQ 112 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 387,653 Number of extensions: 13152 Number of successful extensions: 75 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 75 Number of HSP's successfully gapped: 2 Length of query: 129 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 54 Effective length of database: 1,264,745 Effective search space: 68296230 Effective search space used: 68296230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)