BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0042.0030.p (271 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore rece... 334 4e-93 gi|300310281|ref|YP_003774373.1| TonB-dependent siderophore rece... 196 1e-51 gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisideropho... 78 7e-16 gi|300313794|ref|YP_003777886.1| ferrichrome-iron receptor prote... 60 2e-10 gi|300310762|ref|YP_003774854.1| outer membrane ferric sideropho... 58 6e-10 >gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 334 bits (857), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 158/203 (77%), Positives = 175/203 (86%) Query: 3 LYGGSEPGGTLNVVTRKPQFKAANALDLSIGSNDFYRSAIDSTGALGDNAAYRLDAAVES 62 LYG SEPGGT+NVVT+KPQFK+ANALDLSIGSND YRSA+DSTGAL AYRL+AA+E Sbjct: 159 LYGNSEPGGTINVVTKKPQFKSANALDLSIGSNDAYRSAVDSTGALSSTVAYRLNAAMEH 218 Query: 63 KGGSRDNVRSKREFVAPAFTWLLGNHTTLSYEAEFLRQTAPLDRGIVAVNGRLDALPASR 122 KG RD VRS+REF APAFTWLL +T L+YE EFLRQ APLDRGIVAVNG LP SR Sbjct: 219 KGSMRDQVRSEREFFAPAFTWLLTENTILNYEGEFLRQKAPLDRGIVAVNGNAGVLPVSR 278 Query: 123 FLGEPNDGNIKMDNQNHLFTLEHQFNEQWHLRTGVFHKQGTLDGYSTEASSLRADNATLW 182 FLGEPNDG+IKM NQNHL TLE + NE WHLRTG+FHK+G+LDGYSTEA +L+ADNATL Sbjct: 279 FLGEPNDGDIKMQNQNHLLTLEQRLNENWHLRTGIFHKEGSLDGYSTEAFALQADNATLR 338 Query: 183 RQRRYRDYAWHDTSLQAEIGGKF 205 RQRRYRDY+WHDTSLQAEIGGKF Sbjct: 339 RQRRYRDYSWHDTSLQAEIGGKF 361 Score = 109 bits (272), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 47/62 (75%), Positives = 57/62 (91%) Query: 210 TFMLPAYVTAKALAYWQLTPAMRLSLDVSNLFNKRYYASSYSSVWIAPGDERAATLGLNV 269 +FMLP+YVTA+ALAYWQ TP M+LSLDV+NLFNKRYY+SSY+S+WI PGDER ATL LN+ Sbjct: 644 SFMLPSYVTARALAYWQFTPKMKLSLDVNNLFNKRYYSSSYNSLWIMPGDERNATLALNI 703 Query: 270 RF 271 +F Sbjct: 704 KF 705 >gi|300310281|ref|YP_003774373.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 715 Score = 196 bits (499), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 110/248 (44%), Positives = 151/248 (60%), Gaps = 16/248 (6%) Query: 3 LYGGSEPGGTLNVVTRKPQFKAANALDLSIGSNDFYRSAIDSTGALGDNAAYRLDAAVES 62 LYG SEPGGT+N+VT+KPQF A+N+ ++S G+ R+ +DSTG L N AYRL A E Sbjct: 169 LYGSSEPGGTINIVTKKPQFSASNSAEVSAGTGGLRRATVDSTGPLSQNLAYRLIAMTE- 227 Query: 63 KGGSRDNVRS-KREFVAPAFTWLLGNHTTLSYEAEFLRQTAPLDRGIVAVNGRLDALPAS 121 +G SR ++ S +R VAP+ W++ +T L+YE EFL+ + PLDRGI+ V G L LP Sbjct: 228 EGDSRSSLLSNRRSLVAPSLAWVIDKNTLLNYEGEFLQASTPLDRGIINVRGVLGGLPRD 287 Query: 122 RFLGEPNDGNIKMDNQNHLFTLEHQFNEQWHLRTGVFHKQGTLDGYSTEA---SSLRADN 178 R L EP+DGN+ M + H TLEH +EQW +R G +K+ + DGY TEA + L+AD Sbjct: 288 RVLSEPSDGNMAMRSNTHQLTLEHSLSEQWRMRLGASYKESSFDGYYTEAKLRNPLQADL 347 Query: 179 ATLWRQRRYRDYAWHDTSLQAEIGGKFXPG---ATFMLPAYVTAKALAYWQLTPAMR--- 232 TL R +R D S+Q E+ G+F G TF+ V A+A W T +R Sbjct: 348 RTLDRTGTWRQLPSRDMSIQGELHGQFKTGDIAHTFL----VGAEASKLWMNTEILRSTT 403 Query: 233 -LSLDVSN 239 L +D+ N Sbjct: 404 LLPIDIYN 411 Score = 64.7 bits (156), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 27/60 (45%), Positives = 41/60 (68%) Query: 210 TFMLPAYVTAKALAYWQLTPAMRLSLDVSNLFNKRYYASSYSSVWIAPGDERAATLGLNV 269 T+ LPAY K L+YWQ++ +RLSLDV NLFN+ YY S++++++ PG ER + + Sbjct: 654 TYSLPAYTLVKLLSYWQVSKNVRLSLDVHNLFNRNYYPGSWNNLYVMPGAERTVVARMKI 713 >gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisiderophore receptor signal peptide protein, tonB-dependent receptor [Herbaspirillum seropedicae SmR1] Length = 715 Score = 78.2 bits (191), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 49/171 (28%), Positives = 88/171 (51%), Gaps = 8/171 (4%) Query: 3 LYGGSEPGGTLNVVTRKPQFKAANALDLSIGSNDFYRSAIDSTGALGDNA--AYRLDAAV 60 LYG PGG +N+V+++PQ +D++IGS+D Y+S +D +L +N A+R++ + Sbjct: 167 LYGLVLPGGLVNMVSKRPQGSDFANVDVTIGSHDLYQSTVDLNKSLSENGKQAFRINGLI 226 Query: 61 ESKGGSRDNVRSKREFVAPAFTWLLGNHTTLSY-----EAEFLRQTAPLDRGIVAVNGRL 115 + + D+V K ++AP+ + LG T + E ++RQ G + N Sbjct: 227 SNSDDATDHVWFKNRYIAPSLSLDLGARTDFTILTSYQERNYVRQQGLPLSGSITPNAS- 285 Query: 116 DALPASRFLGEPNDGNIKMDNQNHLFTLEHQFNEQWHLRTGVFHKQGTLDG 166 L S F+GEP + ++L H+F++ W L + ++Q T+ G Sbjct: 286 GPLARSLFIGEPGARPYAANETRIGYSLTHRFDDGWTLNNNLRYQQFTMSG 336 >gi|300313794|ref|YP_003777886.1| ferrichrome-iron receptor protein [Herbaspirillum seropedicae SmR1] Length = 817 Score = 60.1 bits (144), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 41/158 (25%), Positives = 75/158 (47%), Gaps = 10/158 (6%) Query: 3 LYGGSEPGGTLNVVTRKPQFKAANALDLSIGSNDFYRSAIDSTGALGDNA--AYRLDAAV 60 LYG + PGG +NVV++ P A L + +GS+ + D GA+ + YR+ V Sbjct: 258 LYGQAGPGGVVNVVSKLPSADAPRELQVQLGSDRRRQVGADIGGAIDADGRLLYRVIGVV 317 Query: 61 ESKGGSRDNVRSKREFVAPAFTWLLGNHTTLSYEAEFLR-------QTAPLDRGIVAVNG 113 +++ R ++AP+ W + T+L+ +A LR +T PL G + Sbjct: 318 RDAELPAGGMKNDRLYLAPSLVWKISAATSLTLQAALLRDQGGVYTRTRPL-VGSLVPTA 376 Query: 114 RLDALPASRFLGEPNDGNIKMDNQNHLFTLEHQFNEQW 151 +P+ + G P+ + D + + L H+F+E++ Sbjct: 377 IGTTIPSELYTGNPHFDHFSQDQELFGYRLAHRFDERF 414 >gi|300310762|ref|YP_003774854.1| outer membrane ferric siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 717 Score = 58.2 bits (139), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 48/162 (29%), Positives = 73/162 (45%), Gaps = 11/162 (6%) Query: 3 LYGGSEPGGTLNVVTRKPQFKAANALDLSIGSNDFYRSAIDSTGALGDNAAYRLDAAVES 62 LYG GG +N ++++P A+ LS+GS +A+D L + A R++ E Sbjct: 163 LYGRGSGGGVINRISKQPGQDVASQATLSLGSAGLLGAALDWNRPLAPHWALRINTGREH 222 Query: 63 KGGSRDNVRSKREFVAPAFTWLLGNHTTLSYEAEFLRQTAPLDRG-----IVAVNGRLD- 116 G RD V R++ APA W G + L +AE+ DRG I NG Sbjct: 223 AGSFRDTVNGTRQYFAPALKWESGRDSWL-LQAEYDEYERVPDRGIPGRAIATANGEPTA 281 Query: 117 -ALPAS---RFLGEPNDGNIKMDNQNHLFTLEHQFNEQWHLR 154 ALP++ +F G I+ + + TL ++ W LR Sbjct: 282 YALPSAGHEQFFGAAGRDVIRDIHMSLRSTLTRALSQDWTLR 323 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 900,267 Number of extensions: 36197 Number of successful extensions: 123 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 121 Number of HSP's successfully gapped: 8 Length of query: 271 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 187 Effective length of database: 1,222,130 Effective search space: 228538310 Effective search space used: 228538310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)