BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0002.p (662 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309523|ref|YP_003773615.1| bifunctional proline dehydrogen... 620 e-178 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 93 6e-20 gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 76 8e-15 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 76 1e-14 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 76 1e-14 >gi|300309523|ref|YP_003773615.1| bifunctional proline dehydrogenase/proline oxidase delta-1-pyrroline-5-carboxylate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 1230 Score = 620 bits (1598), Expect = e-178, Method: Compositional matrix adjust. Identities = 305/395 (77%), Positives = 334/395 (84%), Gaps = 5/395 (1%) Query: 1 MVRLVKGAYWDSEIKRAQVDGLSGYPVYTRKVHTDLSYLACAQKLLAATDVIYPQFATHN 60 M+RLVKGAYWDSEIKRAQVDGL GYPVYTRKVHTDLSYL CAQKLLAATDVIYPQFATHN Sbjct: 362 MIRLVKGAYWDSEIKRAQVDGLEGYPVYTRKVHTDLSYLTCAQKLLAATDVIYPQFATHN 421 Query: 61 AHTLSAIYTWAQQHKIDDYEFQCLHGMGETLYDQVVGADQLGKACRIYAPVGSHQTLLAY 120 AHTL+AIY WA+QH+ID+YEFQCLHGMGETLYDQVVG D LGKACR+YAPVGSHQTLLAY Sbjct: 422 AHTLAAIYHWARQHQIDNYEFQCLHGMGETLYDQVVGPDNLGKACRVYAPVGSHQTLLAY 481 Query: 121 LVRRLLENGANSSFVNQIVDEAVAIDTLI---TEXPQKSGGEPHPQIALPRNLYGEERKN 177 LVRRLLENGANSSFVNQIVDEAV +D L+ E + GG PHP IA+P LYGEERKN Sbjct: 482 LVRRLLENGANSSFVNQIVDEAVPLDRLVGDPIETVRAQGGLPHPAIAVPHRLYGEERKN 541 Query: 178 STGVDLSNEDTLRGLEHAFAQLSTQQWQAAPLLHDGAVSIGQTQS--ITNPADRRDLVGQ 235 S G+DLSNED L+ L F ++ +QWQAAPLL + + + NPAD R++VGQ Sbjct: 542 SAGIDLSNEDRLQQLGQLFISMADRQWQAAPLLAADTAAQSAQAAQLVRNPADLREVVGQ 601 Query: 236 VIEASRDDVESALHAATEYAVQWQAVSSGDRAQALEHAADLFETHQAELMALAVREAGKS 295 V EA+ DV++AL AAT+YA QWQ+ + +RA LE AADL E H AELMALAVREAGKS Sbjct: 602 VSEATVADVDTALRAATDYAPQWQSTPATERAAMLERAADLLEEHIAELMALAVREAGKS 661 Query: 296 LPNAIAEVREAVDFLRYYATQSRHDGNALAWGPVVCISPWNFPLAIFIGEVSASLAAGNV 355 LPNAIAEVREAVDFLRYYA SRHDGN LAWGPVVCISPWNFPLAIFIGEVSA+LAAGNV Sbjct: 662 LPNAIAEVREAVDFLRYYAIASRHDGNVLAWGPVVCISPWNFPLAIFIGEVSAALAAGNV 721 Query: 356 VLAKPAEQTPLIAHRAVQLLHEAGVPRAALQFLPG 390 VLAKPAEQT LIAHRAVQLLHEAG+PRAALQ LPG Sbjct: 722 VLAKPAEQTALIAHRAVQLLHEAGIPRAALQLLPG 756 Score = 384 bits (985), Expect = e-107, Method: Compositional matrix adjust. Identities = 190/259 (73%), Positives = 214/259 (82%), Gaps = 4/259 (1%) Query: 392 ALRVLCLQEDIADKTMTMLKGAMQELRIGRPDCLATDIGPVIDDEARTQLLAHIDQMKKG 451 ALR+LCLQEDIAD+T+ MLKGAM ELR+GRPD L+ DIGPVID EAR LL HI++M+ Sbjct: 848 ALRILCLQEDIADRTLAMLKGAMAELRVGRPDRLSIDIGPVIDAEARQNLLDHIERMRAS 907 Query: 452 GK-VYQLPLDERHEHGTYVAPTVIEINSIAQLKREVFGPVLHVLRYRRDQLPQLIDTINA 510 + V+QLPL E +HGT+VAPTVIEI+ +AQL+REVFGPVLHVLRYRRD LPQLID INA Sbjct: 908 ARAVHQLPLGEECQHGTFVAPTVIEIDDLAQLQREVFGPVLHVLRYRRDALPQLIDAINA 967 Query: 511 SGYGLTLGVHSRIDETIAFVADRAHVGNIYVNRNIVGAVVGVQPFGGEGKSGTGPKAGGP 570 +GYGLTLGVHSRIDETI FVA RAHVGNIYVNRNIVGAVVGVQPFGGEGKSGTGPKAGGP Sbjct: 968 TGYGLTLGVHSRIDETIEFVAQRAHVGNIYVNRNIVGAVVGVQPFGGEGKSGTGPKAGGP 1027 Query: 571 LYLKRLQRHAEVKLFNDTTPAASEP---LTSLLDWAATHGHAKLAQLGATYVQESLLYKT 627 LYLKRLQR+A++ A P L SLLDWA THGH +LA G Y ++SLL ++ Sbjct: 1028 LYLKRLQRNAQLHEELTRAQPADVPNALLDSLLDWARTHGHERLAANGQRYHRDSLLQRS 1087 Query: 628 LTLPGPTGERNTLSFAPRG 646 L LPGPTGERNTL FAPRG Sbjct: 1088 LVLPGPTGERNTLGFAPRG 1106 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 93.2 bits (230), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 58/192 (30%), Positives = 96/192 (50%), Gaps = 16/192 (8%) Query: 214 AVSIGQTQSITNPADRRDLVGQVIEASRDDVESALHAATEYAVQWQAVSSGDRAQALEHA 273 A + G+T + NPA +++G++ A R D++ AL AA++ W+ +S+ +R++ + A Sbjct: 15 ASASGRTIDVINPATE-EVIGKIAHADRADLDRALEAASKGFETWRNMSAFERSKIMRRA 73 Query: 274 ADLFETHQAELMALAVREAGKSLPNAIAEVREAVDFLRYYATQSRHDGNALA-------- 325 ADL E+ L E GK L A E A D + ++A ++R L Sbjct: 74 ADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPARVPGAY 133 Query: 326 -------WGPVVCISPWNFPLAIFIGEVSASLAAGNVVLAKPAEQTPLIAHRAVQLLHEA 378 GPV +PWNFPL + ++SA+LAAG ++ K E+TP ++ +A Sbjct: 134 QMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAYADA 193 Query: 379 GVPRAALQFLPG 390 GVP + + G Sbjct: 194 GVPAGVINLVYG 205 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 76.3 bits (186), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 69/209 (33%), Positives = 94/209 (44%), Gaps = 17/209 (8%) Query: 199 LSTQQWQAAPLLHDGAVSIGQTQSITNPADRRDLVGQVIEASRDDVESALHAATEYAVQW 258 L + QWQ L V+ Q ++ PA R L QV AS DDV +++ A W Sbjct: 7 LDSAQWQQH-LFRGQWVAAAQGATVLEPATGRPLT-QVGLASPDDVSASVELAIAAQAAW 64 Query: 259 QAVSSGDRAQALEHAADLFETHQAELMALAVREAGKSLPNAIAEVREAVDFLRYYATQS- 317 A+ +RA AA LF H EL + RE G L EVREA+ A Sbjct: 65 VAMPPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPL 124 Query: 318 RHDGNALAWGP-------------VVCISPWNFPLAIFIGEVSASLAAGNVVLAKPAEQT 364 + G L P + ISP+NFPL + + V+ +LAAGN V+ KP +T Sbjct: 125 QAQGQLLPSTPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRT 184 Query: 365 PLI-AHRAVQLLHEAGVPRAALQFLPGXA 392 P+ ++ +AG+P L LPG A Sbjct: 185 PVSGGFMLARIFEQAGLPAGLLHVLPGGA 213 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 75.9 bits (185), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 55/192 (28%), Positives = 89/192 (46%), Gaps = 16/192 (8%) Query: 214 AVSIGQTQSITNPADRRDLVGQVIEASRDDVESALHAAT-EYAVQWQAVSSGDRAQALEH 272 A + G++ + +P+D + + + D++ A+ AA Y W +S+ +R++ L Sbjct: 12 APTSGESIPVLDPSDGQPF-DAIARGNATDIDQAVRAARLAYEGAWGRLSAAERSRLLMK 70 Query: 273 AADLFETHQAELMALAVREAGKSLPNAIAEVREAVDFLRYYATQS-RHDGNALAW----- 326 A + HQ EL L R+ GK + A A+ V + +YA + + G+ + + Sbjct: 71 LAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHGDTIPYQAGYT 130 Query: 327 --------GPVVCISPWNFPLAIFIGEVSASLAAGNVVLAKPAEQTPLIAHRAVQLLHEA 378 G I PWN+P+ IF V +LAAGN + KPAE L R +L E Sbjct: 131 VLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSILRVAELAAEV 190 Query: 379 GVPRAALQFLPG 390 G P AL + G Sbjct: 191 GFPEGALNIVTG 202 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 75.9 bits (185), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 47/138 (34%), Positives = 70/138 (50%), Gaps = 15/138 (10%) Query: 266 RAQALEHAADLFETHQAELMALAVREAGKSLPNAIAEVREAVDFLRYYATQ-SRHDGNAL 324 RA ++ DL HQ +L L RE GK L A EV A ++ ++ + +R +G+ + Sbjct: 75 RAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDII 134 Query: 325 AW--------------GPVVCISPWNFPLAIFIGEVSASLAAGNVVLAKPAEQTPLIAHR 370 G V I+PWNFP A+ +++ +LAAG V+ KPAE TPL + Sbjct: 135 PAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSLA 194 Query: 371 AVQLLHEAGVPRAALQFL 388 V+L EAGVP + + Sbjct: 195 LVRLAQEAGVPVGVINIV 212 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,196,436 Number of extensions: 97240 Number of successful extensions: 368 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 347 Number of HSP's successfully gapped: 21 Length of query: 662 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 571 Effective length of database: 1,188,985 Effective search space: 678910435 Effective search space used: 678910435 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)