BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0038.0064.p (710 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspi... 1316 0.0 gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herb... 117 4e-27 >gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 971 Score = 1316 bits (3406), Expect = 0.0, Method: Compositional matrix adjust. Identities = 626/710 (88%), Positives = 665/710 (93%), Gaps = 3/710 (0%) Query: 1 VDPTDERYAHLVGKMLKLPLSDREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHN 60 VDPTDERYA LVGKM+KLPL REIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHN Sbjct: 265 VDPTDERYAALVGKMIKLPLVGREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHN 324 Query: 61 LEKISIFTLDAKINENGPAKYQGMDRFEARKQIVADLDAAGLLDLVKPHKLMVPRGDRTG 120 LE IS+FTL A INEN P KYQG+DRFEARKQIVADLDA GLL+ VKPHKLMVPRGDRTG Sbjct: 325 LEMISVFTLKATINENAPEKYQGLDRFEARKQIVADLDALGLLEQVKPHKLMVPRGDRTG 384 Query: 121 VVIEPMLTDQWFVAMSKPAPEGTYFPGKSIAEVALEKVANGEIKLIPENWSNTYNQWLNN 180 VVIEPMLTDQWFVAMSKPAPEGT+FPGKSIAE ALEKV++GEIK +PENW+NTYNQWLNN Sbjct: 385 VVIEPMLTDQWFVAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNN 444 Query: 181 IQDWCISRQLWWGHQIPAWYDEDGKVYVARTEAEAQAQAGGKAVTRDNDVLDTWFSSALV 240 IQDWCISRQLWWGHQIPAWYDEDG +YVARTE EA+AQAGG+AV RD DVLDTWFSSALV Sbjct: 445 IQDWCISRQLWWGHQIPAWYDEDGGIYVARTEEEARAQAGGRAVKRDEDVLDTWFSSALV 504 Query: 241 PFSTLGWPEITPDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGQVPFNTVYVHGLVR 300 PFSTLGWPE TPDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTG+VPF+TVYVHGLVR Sbjct: 505 PFSTLGWPEETPDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPFDTVYVHGLVR 564 Query: 301 DASGQKMSKSKGNTLDPIDLIDGIGVDDLVAKRTAGLMNPKQAASIEKATRKEFADGIPA 360 DASGQKMSKSKGNTLDPIDLIDGIGVD+LVAKRT GLMNPKQAASIEKATRKEFADGIPA Sbjct: 565 DASGQKMSKSKGNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEFADGIPA 624 Query: 361 FGTDALRFTFASLATLGRNINFDLGRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGHVK 420 FGTDALRFTFASLATLGRNINFDL RCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGH K Sbjct: 625 FGTDALRFTFASLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGHEK 684 Query: 421 GECNKEKLQFSQADRWIVSLMQRTEAEVEKGFADYRFDNIASAIYKFVWDEYCDWYLEMA 480 G C+KEKLQFS+ADRWIVSL+QRTEAEVEKGFADYRFDNIA+AIYKFVWDEYCDWYLEMA Sbjct: 685 GVCDKEKLQFSKADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWDEYCDWYLEMA 744 Query: 481 KSQIQNGNEAQQRATRRTLLRVLEGVLRLAHPVLPFITEALWQSVAPLTDKKLDPTGDSI 540 K+QIQ+GNEAQQRATRRTLLRVLE VLRLAHPVLPFITE LWQSVAPLTD+K DP GDSI Sbjct: 745 KAQIQSGNEAQQRATRRTLLRVLESVLRLAHPVLPFITEQLWQSVAPLTDRKPDPAGDSI 804 Query: 541 MRQPYPQADLEKIDADAEAWMAQLKTLTDACRNLRGEMQLSPALRVPLIVEAADAADAEA 600 MRQ YP+ DLEKIDA+AEAW+AQ K TDACRNLRGEMQL+P+LRVPLIVEAADA+ A Sbjct: 805 MRQAYPRPDLEKIDAEAEAWVAQFKAYTDACRNLRGEMQLAPSLRVPLIVEAADAS---A 861 Query: 601 VNSFLPYLQTLVKLSEAQVVTLLPESPAPVAIVGDLKLMLKVEIDVAAERERMAKEITRI 660 V SFLPYLQ LV+LSE VV LPESPAPVAIVG++KLMLKVEIDVAAERER+ KE+TR+ Sbjct: 862 VESFLPYLQGLVRLSETTVVAQLPESPAPVAIVGEVKLMLKVEIDVAAERERLGKEVTRL 921 Query: 661 SGEIVKANAKLSNESFVARAPEAVVAQEKERLANFTTTLAKLQEQLAKLK 710 + EI KAN KLSNESFVARAPEAVVAQEKER+ANF+ TL K++EQLAKLK Sbjct: 922 TAEIAKANGKLSNESFVARAPEAVVAQEKERVANFSATLEKVKEQLAKLK 971 >gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 964 Score = 117 bits (292), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 134/581 (23%), Positives = 231/581 (39%), Gaps = 99/581 (17%) Query: 22 DREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEKISIFTLDAKINENGPAK- 80 DR P+ EYV + GTG V +PA+ D+ H ++ I L+ + + A Sbjct: 336 DRLSPVFLGEYVTADSGTGIVHSSPAYGIEDFVSCKAHGMKDDDI--LNPVMGDGRYASW 393 Query: 81 ---YQGMDRFEARKQIVADLDAAG-LLDLVK-PHKLMVPRGDRTGVVIEPMLTDQWFVAM 135 + G+ +EA K I L+ G L L+ H M ++ ++ T QWF +M Sbjct: 394 LPLFAGLTIWEASKPICDKLEEIGSLFKLIMFSHSYMHCWRHKSPIIYRA--TSQWFASM 451 Query: 136 SKPAPEGTYFPGKSIAEVALEKVANGEIKLIPENWSNTYNQWLNNIQDWCISRQLWWGHQ 195 +G G S+ + AL+ + + P + + N DW +SRQ WG Sbjct: 452 DNTPKDG----GPSLRQTALQGIED--TAFFPGWGKARLHGMIANRPDWTLSRQRQWGVP 505 Query: 196 IPAW-YDEDGKVYVARTE-----AEAQAQAGGKA----------------VTRDNDVLDT 233 + + + E G+++ E A+ Q G +A ++ D LD Sbjct: 506 MAFFVHKETGELHPRTPELLEQIAQRIEQGGIEAWQALDPKELLGDEADQYLKNKDTLDV 565 Query: 234 WFSSALVPFSTLGWPEITPDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGQVPFNTV 293 WF S + L + +L P+ + + G D W ++ ++ G+ P+ + Sbjct: 566 WFDSGSTHQTVL---RGSHRQELQFPADLYLEGSDQHRGWFHSSLLTSSMLNGRAPYKAL 622 Query: 294 YVHGLVRDASGQKMSKSKGNTLDPIDLIDGIGVDDLVAKRTAGLMNPKQAASIEKATRKE 353 HG V D G+KMSKS N ++P + D +G + L L S E + +E Sbjct: 623 LTHGFVVDGEGRKMSKSLKNGVEPQKVADSLGAEILR------LWVASSDYSGEISISEE 676 Query: 354 FADGIPAFGTDALRFTFASLATLGRNINFDLGRCEGYRNFCNKLWNATRFVLMNTEGQDC 413 + E YR N L RF+L NT Sbjct: 677 ILKRV----------------------------TEAYRRIRNTL----RFLLANT----S 700 Query: 414 GFDGHVKGECNKEKLQFSQADRWIVSLMQRTEAEVEKGFADYRFDNIASAIYKFVWDEYC 473 FD E L+ DR+ ++ M R + EV F Y F + + + + ++ Sbjct: 701 DFDPAQHAVPMAELLEI---DRYAIANMARLQGEVLAHFETYEFHPVVAKLQMYCSEDLG 757 Query: 474 DWYLEMAKSQIQNG--NEAQQRATRRTLLRVLEGVLRLAHPVLPFITEALWQSVAPLTDK 531 +YL++ K ++ + A +R+ + + + + +LRL P+L F E W A + Sbjct: 758 GFYLDILKDRLYTSGVDSAARRSAQTAVWHITQSLLRLMAPILSFTAEEAWSFFA--GKE 815 Query: 532 KLDPTGDSIMRQPY---PQADLEKIDADAEAWMAQLKTLTD 569 + +G++I Q Y P+ ADA+ +A+ L + Sbjct: 816 AFEASGETIFSQVYYALPEV------ADAQGLLAKFAVLHE 850 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,010,209 Number of extensions: 77820 Number of successful extensions: 216 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 2 Length of query: 710 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 618 Effective length of database: 1,184,250 Effective search space: 731866500 Effective search space used: 731866500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)