BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0038.0058.p (348 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subun... 496 e-141 gi|300313125|ref|YP_003777217.1| 1-deoxy-D-xylulose 5-phosphate ... 131 6e-32 gi|300309646|ref|YP_003773738.1| transketolase C-terminal subuni... 89 4e-19 gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 63 5e-11 >gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 334 Score = 496 bits (1276), Expect = e-141, Method: Compositional matrix adjust. Identities = 239/329 (72%), Positives = 271/329 (82%) Query: 20 APAKPRLTTSAMIASLAAEGQATRPAPFGHALAELANVRTDIVGMTADLAKYTDLHIFAK 79 A KPRLTTSAMIAS+AAEGQ + APFG AL + A R +IVG+TADLAKYTDLH+F K Sbjct: 5 ADKKPRLTTSAMIASIAAEGQRVQAAPFGKALVDYAADRPEIVGLTADLAKYTDLHLFQK 64 Query: 80 EHPERFYEMGMAEQLLMGAAAGMAREGMVPFATTYAVFASRRAYDFICMAIAEESLNVKI 139 +PERF++MGMAEQLLM AA GMA+EG+VPFATTYAVFA+RRAYDFI IAEE+LNVKI Sbjct: 65 AYPERFFQMGMAEQLLMAAAGGMAKEGLVPFATTYAVFATRRAYDFIHQVIAEENLNVKI 124 Query: 140 VAALPGLTTGYGPSHQATDDLAIFRAMPNLTIVDPCDALDIEQAVPQIADHRGPVYMRLL 199 ALPGLTTGYGPSHQAT+DLA+ RA+P +TIVDPCDALD+EQAVPQIA H+GPVYMRL Sbjct: 125 CCALPGLTTGYGPSHQATEDLAMMRAIPGMTIVDPCDALDLEQAVPQIAAHQGPVYMRLA 184 Query: 200 RGNVPAVLDEYNYKFELGKAKMIRDGRDVLIISTGLMTMRALEAAKLLQKDTVDVGVLHV 259 RGNVP VLDEY+Y+FELG+AK IRDG DVLIIS+G MTMRALE AK L+ D V V VLH Sbjct: 185 RGNVPLVLDEYDYRFELGRAKEIRDGNDVLIISSGFMTMRALEVAKALEADKVSVAVLHC 244 Query: 260 PTIKPLDEETIRKEAARSGRMVIVAENHSVVGGLGEAVAGVLMRANVRPPAFRQIGLPDA 319 PTIKP DE T+ + + R+V+VAENHSVVGGLGE VA LMR V+P F+ GLPDA Sbjct: 245 PTIKPFDEATVVAQVKKKNRLVVVAENHSVVGGLGEIVATTLMRHRVQPAGFQLAGLPDA 304 Query: 320 FLDAGALPTLHDRYGISAAVMSANIKSWL 348 FLDAGALPTLHDRYGIS V+ IK WL Sbjct: 305 FLDAGALPTLHDRYGISCEVLGRRIKEWL 333 >gi|300313125|ref|YP_003777217.1| 1-deoxy-D-xylulose 5-phosphate synthase [Herbaspirillum seropedicae SmR1] Length = 621 Score = 131 bits (330), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 97/310 (31%), Positives = 160/310 (51%), Gaps = 9/310 (2%) Query: 36 AAEGQATRPAPFGHALAELANVRTDIVGMTADLAKYTDLHIFAKEHPERFYEMGMAEQLL 95 +A G+ T FG L ++A +VG+T + + + + F K+ P R+Y++G+AEQ Sbjct: 304 SAGGKLTYTKVFGDWLCDMAAADDKLVGITPAMREGSGMVDFEKKFPNRYYDVGIAEQHA 363 Query: 96 MGAAAGMAREGMVPFATTYAVFASRRAYDFICMAIAEESLNVKIVAALPGLTTGYGPSHQ 155 + AAGMA EGM P Y+ F +RAYD + +A ++L+V GL G +H Sbjct: 364 VTFAAGMACEGMKPVVAIYSTFL-QRAYDQLIHDVALQNLDVTFALDRAGLVGADGATHA 422 Query: 156 ATDDLAIFRAMPNLTIVDPCDALDIEQAVPQIADHRGPVYMRLLRG-NVPAVLDEYNYKF 214 DLA R +PN+ ++ D + + + + GP +R RG + A +D+ Sbjct: 423 GNYDLAFLRCIPNMVVMAASDENECRKMLSTAYQYPGPASVRYPRGAGIGAAVDQTLETL 482 Query: 215 ELGKAKMIRDGRDVLIISTGLMTMRALEAAKLLQKDTVDVGVLHVPTIKPLDEETIRKEA 274 LG+ ++ R G++V I++ G +L A L D ++ V ++ IKPLD E +++ A Sbjct: 483 PLGRGEIRRQGKNVAILAFG-----SLLAPSLGAGDKLNATVANMRFIKPLDVELVKQLA 537 Query: 275 ARSGRMVIVAENHSVVGGLGEAVAGVLMRANVRPPAFRQIGLPDAFLDAGALPTLHDRYG 334 A +V V E ++GG G AV+ L A + P +GLPD F+D G L + G Sbjct: 538 ASHDALVTVEEG-CIMGGAGAAVSEALAAAGINKPIL-HLGLPDRFIDHGDAAQLLAQCG 595 Query: 335 ISAAVMSANI 344 + A ++ +I Sbjct: 596 LDANGIADSI 605 >gi|300309646|ref|YP_003773738.1| transketolase C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 321 Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 67/278 (24%), Positives = 131/278 (47%), Gaps = 16/278 (5%) Query: 80 EHPERFYEMGMAEQLLMGAAAGMAREGMVPFATTYAVFASRRAYDFICMAIAEES----L 135 + P+RF +G+AEQ L+ +AG+A EG FA A F + R Y+ + +++A S + Sbjct: 44 DFPDRFVNVGIAEQNLINVSAGLALEGYKVFAYAIAPFITMRCYEQVRVSLALLSEVRPM 103 Query: 136 NVKIVAALPGLTTGY-----GPSHQATDDLAIFRAMPNLTIVDPCDALDIEQAVPQIADH 190 NV ++ G+ GY GP+HQ +D+ + RA+PN ++ D + ++ Sbjct: 104 NVNLI----GVGAGYSYVVSGPTHQCYEDITLMRALPNFQVLSAADHVTAAAFFDTCSNS 159 Query: 191 RGPVYMRLLRGNVPAVLDEYNYKFELGKAKMIRDGRDVLIISTGLMTMRALEAAKLLQKD 250 GP Y+R +P + + G + R G + +++TG M AL+AA++L + Sbjct: 160 MGPKYLRFDAQVLPVIYGDGAPDLAQG-FHIHRRGGKIGLVATGYMLHTALQAAEVLAAE 218 Query: 251 TVDVGVLHVPTIKPLDEETIRKEAARSGRMVIVAENHSVVGGLGEAVAGVLMRANVRPPA 310 ++VGV+ + + + +++ + + E GG+ + + R ++ Sbjct: 219 GIEVGVIDLFDLARFSADALQETLQSYAGIATLEEGFRGRGGMDSMLFEFIARRGLQTRM 278 Query: 311 FRQIGLPDAF-LDAGALPTLHDRYGISAAVMSANIKSW 347 IG+ + + G LH+ GI + +I+ + Sbjct: 279 L-NIGVDGVYRFELGTRTELHEEVGIGPQAVLTSIREF 315 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 62.8 bits (151), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 59/215 (27%), Positives = 94/215 (43%), Gaps = 13/215 (6%) Query: 64 MTADLAKYTDLHIF-AKEHPERFYEMGMAEQLLMGAAAGMAREG-MVPFATTYAVFASRR 121 + A A LH F A + + G+ E L+ A GMA G ++ + TY F+ Sbjct: 378 LEAPTAHKGGLHAFTADDRSGAYVHCGVREHLMGAMANGMAAHGGVIALSVTYLAFSD-- 435 Query: 122 AYDFICMAI-AEESLNVKIVAALPGLTTGY-GPSHQATDDLAIFRAMPNLTIVDPCDALD 179 Y+ M + A L VK V + + G GP+HQ + LA RAMPN+ ++ P DA + Sbjct: 436 -YERPAMRMSALMGLPVKYVFSHDSIGIGKNGPTHQPVEILASLRAMPNMLVMRPADATE 494 Query: 180 IEQAVPQIADHR-GPVYMRLLRGNVPAVLDEYNYKFELGKAKMIRD-----GRDVLIIST 233 + HR GPV M R +P V +Y + + + R V +++T Sbjct: 495 AVECWEIAMQHRSGPVSMVFARQALPPVRRQYEAENRCARGGYVLHEAQCGARQVTLLAT 554 Query: 234 GLMTMRALEAAKLLQKDTVDVGVLHVPTIKPLDEE 268 G A A L+ + V+ +P + D++ Sbjct: 555 GSEVALACAARAQLEAAGIATAVVSLPCWELFDQQ 589 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 925,610 Number of extensions: 33654 Number of successful extensions: 129 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 126 Number of HSP's successfully gapped: 4 Length of query: 348 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 262 Effective length of database: 1,212,660 Effective search space: 317716920 Effective search space used: 317716920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)