BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0038.0035.p (384 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313395|ref|YP_003777487.1| porin transmembrane protein [He... 405 e-114 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 139 5e-34 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 137 1e-33 gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 137 2e-33 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 129 3e-31 >gi|300313395|ref|YP_003777487.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 405 bits (1042), Expect = e-114, Method: Compositional matrix adjust. Identities = 210/371 (56%), Positives = 271/371 (73%), Gaps = 3/371 (0%) Query: 17 AQSSVTIYGALDNALVYTNNQGGLHNLYLRTGNLAASKIGFKGAEDLGGGSQAIFTLENG 76 AQSSVT+YG +D L Y++NQGG N Y+ +G LAASK+G G EDLGGG+ A+F LENG Sbjct: 33 AQSSVTLYGTVDLGLAYSSNQGGASNTYMNSGALAASKLGLTGTEDLGGGNMALFRLENG 92 Query: 77 FDINTGAMSSANTLFNRQSFVGLTNSGYGTITTGRQYTPYFLYVGAIGPTSVLTGATGAH 136 F+ +TGA S+A LFNRQS+VGL++ YG +T GRQYTPYF YVGA+GPT+VLTGATGAH Sbjct: 93 FNADTGAQSTAGVLFNRQSYVGLSSKDYGQVTVGRQYTPYFQYVGALGPTNVLTGATGAH 152 Query: 137 PGDVDGLDTTVRISNSVTYSSPVFGGAQVSALYGFGETAGSVSTGNTYSLAFKYDVSAWN 196 PGD+D LDTT+R++NS+TY+ P GG Q YG GE AGS + G++ S A +YD A++ Sbjct: 153 PGDIDALDTTLRMNNSITYTLPTLGGLQAGVQYGMGEQAGSAANGSSVSAALRYDYQAFS 212 Query: 197 FALGYQRLKNGSGPANAWNSTASGSFS-TSAINNGYVSSDNVQMIAGALRYTIDKWMVGA 255 ++ GY RLKN + A + GSF+ S +N GY S+D+ Q++A A RYT M+G Sbjct: 213 WSAGYTRLKNLANGNTAGSFGNVGSFANNSPVNAGYASADSTQLLATAARYTRGDLMLGL 272 Query: 256 NYANAQYKPGAGSLFTQKATFQTLGLITTYQLTPQVALAGGYSYTFEKSANGISDAAKYH 315 NY+N QYK GA S F+Q A F T+GLI +Y ++P + LA GYSYT EK+ NGIS AKY+ Sbjct: 273 NYSNVQYKAGALSAFSQTAVFNTIGLIGSYNVSPALVLAAGYSYTAEKARNGISSPAKYN 332 Query: 316 QIALEQTYSFSKRSAIYFLEAYQLARGKTL--GKTAGSIVDAVAVVGDSQNGSPSSGRNQ 373 Q++LE+ YS SKR+A+Y ++AYQ A G+TL G SIV+AVA VGDSQNG+PSSG Q Sbjct: 333 QLSLEEVYSLSKRTALYAIQAYQRASGQTLRAGGVGSSIVNAVASVGDSQNGTPSSGSGQ 392 Query: 374 SVLMVGFRHLF 384 V M+G RH F Sbjct: 393 FVAMLGIRHSF 403 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 139 bits (349), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 130/400 (32%), Positives = 183/400 (45%), Gaps = 73/400 (18%) Query: 4 LAIVAAGASVQASAQSSVTIYGALDNALVYTNNQ-----GGLHNLYLRTGNLAASKIGFK 58 +A + AG + A AQSSV+IYG +D L Y ++ GG L + +G S+IGFK Sbjct: 7 VAAMLAGGATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQGSRIGFK 66 Query: 59 GAEDLGGGSQAIFTLENGFDINTGAMSSANTLFNRQSFVGLTNSGYGTITTGRQYTPYFL 118 G EDLGGG A FTLE GF + G++ LF RQSFVGL ++ +G+++ GRQY F+ Sbjct: 67 GTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQYD--FM 124 Query: 119 -------YVGAIGPTSVLTGATGAHPGDVDGLDTTV----RISNSVTYSSPVFGGAQVSA 167 +GA+ P A L+ + R +NS+ Y+S FGG A Sbjct: 125 ANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSFGA 184 Query: 168 LYGFGETAGSVSTGNTYSLAFKYDVSAWNFALGYQRLKNGSGPANAWNSTASGSFSTSAI 227 + G GETAG+ + G T S +YD ++ AL Y +K+ ++GSFST Sbjct: 185 MVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTDIKD-----------STGSFST--- 230 Query: 228 NNGYVSSDNVQMIAGALRYTIDKWM---VGANYANAQYKPGAGSLFTQKATFQTLGLITT 284 ++ G RY I + V N + KAT LG T Sbjct: 231 ----------RIYGGGARYQIGAFTPFGVVTQVKNTNT--------SAKATTYELG--TV 270 Query: 285 YQLTPQVALAGGYSYTFEKSANGISDAAKYHQIALEQTYSFSKRSAIYFLEAYQLARGKT 344 Y LTP LA +Y F+K G+ +A + Y SKR+ +Y Y +G Sbjct: 271 YALTPLWDLAA--AYQFQKRNQGVGNA---QALVATVDYKISKRTDLYVGAVYDRDKG-- 323 Query: 345 LGKTAGSIVDAVAVVGDSQNGSPSSGRNQSVLMVGFRHLF 384 +A V G G S+ NQ+ L +G RH F Sbjct: 324 --------YNAYPVFG---GGIQSATGNQTALRLGMRHRF 352 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 137 bits (345), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 128/393 (32%), Positives = 184/393 (46%), Gaps = 51/393 (12%) Query: 19 SSVTIYGALDNALVY-----TNNQGGLHNLYLRTGNLAASKIGFKGAEDLGGGSQAIFTL 73 SSVTIYG +D +VY T G L +G + S+IGFKG EDLGGG A+F L Sbjct: 22 SSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQGSRIGFKGTEDLGGGLSAVFNL 81 Query: 74 ENGFDINTGAMS---SANTLFNRQSFVGLTNSGYGTITTGRQYTPYFLYVGAIGPTSVLT 130 E GF +TGA+ ++NTLF R+S VGL + +G++ GRQ T + + + Sbjct: 82 EAGFSNDTGALDDSKTSNTLFRRKSVVGLAGN-FGSVLLGRQ-TDFADTISSYTSVQDFG 139 Query: 131 GATGAHPGDVDGLDTTVRISNSVTYSSPVFGGAQVSALYGFGETAGSVSTGNTYSLAFKY 190 G TGA ++D L+ T R +NS++Y++ G + +YGFGETAG S G + + KY Sbjct: 140 GWTGAAGHNLDRLEGT-RTNNSISYTTANLNGFTGNLMYGFGETAGQTSAGQAFGVGGKY 198 Query: 191 DVSAWNFALGYQRLKNGSGPANAWNSTASGSFS-TSAINNGYVSSDNVQMIAGALRYTID 249 D + Y + K GS PA+A A+G+ T+ NNG + +++ A Y Sbjct: 199 DNGPLGLGINYYQSKAGSTPADASLLAATGTNQFTNGTNNGSTAQ---KVLNVAASYQFG 255 Query: 250 KWMVGANYANAQYK---------PGAGSLFT---------QKATFQTLGLITTYQLTPQV 291 V N++ + G GSL T KA +G T Y L+P + Sbjct: 256 PARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTLAASNKADIYEIG--TAYSLSPSL 313 Query: 292 ALAGGYSYTFEKSANGISDAAKYHQIALEQTYSFSKRSAIYFLEAYQLARGKTLGKTAGS 351 L +T +G+S AK Q++L Y SKR+ +Y AY K Sbjct: 314 KLLAAVDHT-RAQFDGVSGKAKLTQVSLGTDYWLSKRTDLYAFLAYS----KATDLNNPG 368 Query: 352 IVDAVAVVGDSQNGSPSSGRNQSVLMVGFRHLF 384 + D+ G NQ + VG RH F Sbjct: 369 VADSSGTDG-----------NQGAVAVGIRHKF 390 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 137 bits (345), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 124/381 (32%), Positives = 178/381 (46%), Gaps = 36/381 (9%) Query: 15 ASAQSSVTIYGALDNALVYTNNQG--GLHNLYLRTGNLAASKIGFKGAEDLGGGSQAIFT 72 A AQS+VTIYG +D ++ Y +N G N+ + G +A S+IGFKG EDLGGG +A+F Sbjct: 19 AFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSRIGFKGTEDLGGGLKAMFQ 78 Query: 73 LENGFDINTGAMSSANTLFNRQSFVGLTNSGYGTITTGRQYTPYF-LYVGAIGPTSVLTG 131 LENGF +TGAM +LF RQS+VGL G+G + GRQ TP F L P +V Sbjct: 79 LENGFSSDTGAMQQGGSLFGRQSWVGL-QGGFGKVRVGRQNTPLFDLLADHFDPLTVGNY 137 Query: 132 ATGAHPGDVDGLDTTVRISNSVTYSSPVFGGAQVSALYGFGETAGSVSTGNTYSLAFKYD 191 A+ + + + VR N + Y FG SA + FGE AGSV TG+ +S A +Y Sbjct: 138 ASNSW---LPAAASLVRSPNMLRYDGD-FGPVAASASWAFGEKAGSVRTGSQFSSALRYT 193 Query: 192 VSAWNFALGYQRL---KNGSGPANAWNSTASGSFSTSAINNGYVSSDNVQMIAGALRYTI 248 GYQ+ N S WN +AS F + + G+++ + + G++ Sbjct: 194 AGPLALGGGYQQTVSSTNSSLKHTTWNLSASYDFGVAKVFGGWLNIKDDSGLTGSVMAAD 253 Query: 249 DKWMVGANYANAQYKPGAGSLFTQKATFQTLGLITTYQLTPQVALAGGYSYTFEK----- 303 G A A G F L ++Y+ T + L G + Y K Sbjct: 254 GDVSNGWTTAGAANNGSTGVKRKDNGFF----LGSSYKATNALTLTGAFYYDRSKNVSYV 309 Query: 304 SANGISDAAKYHQIALEQTYSFSKRSAIYFLEAYQLARGKTLGKTAGSIVDAVAVVGDSQ 363 S + D +Y + Y+ SKR+ +Y Y A+ AV + +S Sbjct: 310 STGNVGDGKRYTLVGWAD-YALSKRTQLYTTIDYNKAKDA-----------AVYELTNSA 357 Query: 364 NGSPSSGRNQSVLMVGFRHLF 384 NG S + + VG RH+F Sbjct: 358 NGKNS----LTTVGVGIRHIF 374 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 129 bits (325), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 130/425 (30%), Positives = 190/425 (44%), Gaps = 71/425 (16%) Query: 4 LAIVAAGASVQASAQSSVTIYGALDNALVYTN-------NQGGLHNLYLRTGNLAASKIG 56 LA++ A A + SS+TIYGA+D +L YTN N G L + +G + S++G Sbjct: 8 LAVLGAFAGAAHAQSSSITIYGAIDASLTYTNKIATDGKNTGSL--TAVESGLVKGSRLG 65 Query: 57 FKGAEDLGGGSQAIFTLENGFDINTGAMSSANTLFNRQSFVGLTNSGYGTITTGRQYTPY 116 KG+EDLGGG +A+FTLENGF+++TG ++ RQ+ VGL+ + +GT+ GRQ P Sbjct: 66 IKGSEDLGGGLKAVFTLENGFNVDTGEAGQGKLMWGRQTTVGLSGN-FGTVLVGRQNDP- 123 Query: 117 FLYVGAIGPTSVLTGATGAHPGDV--DGLDTT--VRISNSVTYSSPVFGGAQVSALYGFG 172 +G I G G PG V GLD T R++NS+ Y+SP F G S +YGFG Sbjct: 124 LNDIGGIASA----GGFGV-PGTVHSKGLDRTDGQRLNNSIRYNSPNFNGLSFSGIYGFG 178 Query: 173 ETAGSVSTGNTYSLAFKYDVSAWNFALGYQRLK-----NGSGPANA-------------- 213 E G G + L +Y + LGY + K +GS NA Sbjct: 179 EQVGRAVGGRSIGLGSQYVNGPLSLGLGYFQSKMGGGSDGSSDVNAKAACNGAGEKDHAG 238 Query: 214 ------WNSTASGSFSTSAINNGYVSSDNVQMIAGALRYTIDKWM-VGANY---ANAQYK 263 W A+ F + + Y + +V++ L + K GA Y Y Sbjct: 239 DTCLKTWTLAAAYQFGPAKV---YGAFSHVRLPLAKLHTKVRKDNNFGAMYDAKTGTGYN 295 Query: 264 PGAGSLFT----QKATFQTLGLITTYQLTPQVALAGGYSYTFEKSANGISDAAKYHQIAL 319 P + F+ + QT + +YQL P + L +T K S + Q+ L Sbjct: 296 PSDATAFSIGGANHKSSQTFDIGMSYQLMPSLTLMSAVQHTRAKFVGAAS--GRLLQLNL 353 Query: 320 EQTYSFSKRSAIYFLEAYQLARGKTLGKTAGSIVDAVAVVGDSQNGSPSSGRNQSVLMVG 379 Y SKR++ AY L R + VG S++ P R+Q+ + G Sbjct: 354 GAKYDLSKRTS-----AYALVRNLR--------SSDMYNVGLSKDQEPGIDRSQTAISTG 400 Query: 380 FRHLF 384 H F Sbjct: 401 LIHTF 405 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.130 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,195,861 Number of extensions: 51973 Number of successful extensions: 170 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 14 Length of query: 384 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 297 Effective length of database: 1,207,925 Effective search space: 358753725 Effective search space used: 358753725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)