BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0038.0022.p (385 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 581 e-167 gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 109 4e-25 gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 78 1e-15 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 56 6e-09 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 581 bits (1497), Expect = e-167, Method: Compositional matrix adjust. Identities = 299/431 (69%), Positives = 339/431 (78%), Gaps = 46/431 (10%) Query: 1 MHSLIALDGDDRLLSNSITWGDIRASVWAERIKNEFDGQAIYRRTGTPIHPMSPLCKLMW 60 MHS+IALD ++RLLSNSITWGDIRASVWAERIK+E DG AIYRRTGTP+HPMSPLCKLMW Sbjct: 87 MHSVIALDAENRLLSNSITWGDIRASVWAERIKHEHDGNAIYRRTGTPVHPMSPLCKLMW 146 Query: 61 MRHEKAAVFNQAARFVGIKEYVLFRLFGEWLVDYSIASATGMFNLEQLEWDEGALALLHV 120 MRHEK VF++AARFVGIKEY+L++LFG+W+VD+SIASATGMFNL +L WD+GALALL V Sbjct: 147 MRHEKPDVFHRAARFVGIKEYLLYQLFGQWVVDHSIASATGMFNLRELAWDQGALALLGV 206 Query: 121 GKHQLPKPVPTTHQLQGLDAAIAERLGXK------------------------------- 149 QLP PVPTTH+L L + +A+RLG Sbjct: 207 RPDQLPTPVPTTHRLPALSSEMAQRLGLSVDTPFIIGANDGVLSNLGVNAIEIGHVAVTI 266 Query: 150 -----------KKKKPP----FCYALTERHWVVGGPVNNGGNVFRWVRDELATAEAEQAR 194 + + P FCYALTE+HWVVGGPVNNGGN+FRWVRDELATAEA AR Sbjct: 267 GTSGAMRTVIDEPRTDPQGRLFCYALTEKHWVVGGPVNNGGNIFRWVRDELATAEAAAAR 326 Query: 195 KDGVDPYDALTRIAEHIAPGAEGLLFHPYLSGERAPLWNADLRGSFFGLALHHRKEHMIR 254 ++GVDPY+ALTRIAE + PGAEGLLFHP+++GERAPLWNADLRGSFFGLALHH K HMIR Sbjct: 327 EEGVDPYEALTRIAEKVRPGAEGLLFHPFMAGERAPLWNADLRGSFFGLALHHGKHHMIR 386 Query: 255 AALEGVIFNLYSILPAVEDLIGPTRRMMATGGFARSALWRQMMADIFNREVVVPQSVESS 314 AALEGVIFNLYSILPA+E+L+GPT+RMMATGGFARSALWRQMMADIFNREVVVP+SVESS Sbjct: 387 AALEGVIFNLYSILPALEELVGPTKRMMATGGFARSALWRQMMADIFNREVVVPESVESS 446 Query: 315 CLGAAVLGLFALGKVKSLDVISDMVGSTNRHLPIPENVAIYDRLRPIFMAIPHKLADEYR 374 CLGAAVLG +ALG V SL VIS MVGSTN H P E VA+Y RL+PIF AIP KL EY Sbjct: 447 CLGAAVLGAWALGLVPSLSVISGMVGSTNHHAPEAEAVAVYGRLQPIFAAIPAKLEAEYH 506 Query: 375 ALSAFQRRDVR 385 A++AFQR R Sbjct: 507 AIAAFQREAGR 517 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 109 bits (272), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 103/377 (27%), Positives = 154/377 (40%), Gaps = 69/377 (18%) Query: 16 NSITWGDIRASVWAERIKNEFDGQAIYRRTGTPIHPMSPLCKLMWMRHEKAAVFNQAAR- 74 N++ W D RA V ++ G+AI ++TG + P KL W+ + +A R Sbjct: 97 NAVVWQDRRAEVVCAALRERGLGEAIQKKTGLVLDPYFSGTKLRWILDHVDGLRARAERG 156 Query: 75 ---FVGIKEYVLFRLFGEWL--VDYSIASATGMFNLEQLEWDEGALALLHVGKHQLPKPV 129 F + ++++ L G L D S AS T ++N+ WDE L L + LP+ Sbjct: 157 ELLFGTVDSWLVWNLTGGRLHVSDVSNASRTMLWNIHTGAWDEELLQWLGIPAAMLPQVQ 216 Query: 130 PTTH-----QLQGLDAAI-----------------AERLGXKKKKKPPFCYAL------- 160 P+ H Q L AA+ R G K C+ L Sbjct: 217 PSAHLYGEIDAQHLGAAVKIGGIAGDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQC 276 Query: 161 ----------------TERHWVVGGPVNNGGNVFRWVRDELATAEAEQARKDGVDPYDAL 204 ++ + + G V GG V +W+RD L +A K D Sbjct: 277 ATSHNGLISTAACQVDEQKQYALEGSVFIGGAVVQWLRDGL------KAIKTSTD----- 325 Query: 205 TRIAEHIAPGAE---GLLFHPYLSGERAPLWNADLRGSFFGLALHHRKEHMIRAALEGVI 261 E +A E G++F P +G AP WN +G+ GL+ H+ RAALE + Sbjct: 326 ---VEGLACSVEDSGGVVFVPSFTGLGAPYWNPSAKGAIVGLSRGSTVAHIARAALESIA 382 Query: 262 FNLYSILPA-VEDLIGPTRRMMATGGFARSALWRQMMADIFNREVVVPQSVESSCLGAAV 320 F ++L A V D + P + GG A + L Q AD+ V+ PQ E++ LGAA Sbjct: 383 FQSTALLNAMVRDAVSPITELRVDGGAAANNLLLQFQADLLGIPVIRPQVTETTALGAAY 442 Query: 321 LGLFALGKVKSLDVISD 337 L A G + L +S Sbjct: 443 LAGIATGVYRDLSELSS 459 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 77.8 bits (190), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 98/382 (25%), Positives = 148/382 (38%), Gaps = 74/382 (19%) Query: 1 MHSLIALDGDDRLLSNSITWGDIRASVWAERIKNEFDGQAIYRRTGTPIHPMSPLCKLMW 60 MH + LD D +L +I W D R++ E + E ++ G P KL+W Sbjct: 80 MHGAVLLDERDEVLRPAILWSDSRSA--PECAELESRAPRLHGIAGNLAMPGFTAPKLLW 137 Query: 61 MRHEKAAVFNQAARFVGIKEYVLFRLFGEWLVDYSIASATGMFNLEQLEWDEGALALLHV 120 + + +F + A + K+++ ++ G + D S A+ T ++E W + LA + Sbjct: 138 VARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWLDVEGRNWSDELLAASGM 197 Query: 121 GKHQLPKPV----------PTTHQLQGLDAAI------------AERLGXKK-------- 150 + Q+P V P Q GL + + A +G K Sbjct: 198 RRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVIVAGGAGDGAASAVGIGAVKPGDGFLSL 257 Query: 151 -------------KKKP-----PFCYALTERHWVVGGPVNNGGNVFRW------VRDELA 186 + P FC+ L +R W + + + RW V ++ Sbjct: 258 GTSGVLFVVNDRFRPNPGRAIHAFCHTLPQR-WHQMSVMLSAASCLRWFCRLCSVDEKSL 316 Query: 187 TAEAEQARKDGVDPYDALTRIAEHIAPGAEGLLFHPYLSGERAPLWNADLRGSFFGLALH 246 AE EQ L A + AP LF PYLSGER P + G F GL Sbjct: 317 LAEIEQ-----------LDEQACNNAP-----LFLPYLSGERTPHNDPYATGVFHGLTPE 360 Query: 247 HRKEHMIRAALEGVIFNLYSILPAVEDLIGPTRRMMATGGFARSALWRQMMADIFNREVV 306 H++ + A LEGV F + L A+ + GG ARSA W Q++AD +V Sbjct: 361 HQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIV 420 Query: 307 VPQSVES-SCLGAAVLGLFALG 327 E+ LGAA L A G Sbjct: 421 THVGSEAGGALGAARLAWLADG 442 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 55.8 bits (133), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 42/129 (32%), Positives = 58/129 (44%), Gaps = 3/129 (2%) Query: 200 PYDALTRIAEHIAPG--AEGLLFHPYLSGERAPLWNADLRGSFFGLALHHRKEHMIRAAL 257 P L AE ++ G A+ LF PYL+GER P +A +G FG+ H H+ A + Sbjct: 312 PVAELVSGAERLSAGQQAQAPLFLPYLNGERTPHNDAAAKGVLFGMTPAHESAHLAYAVM 371 Query: 258 EGVIFNLYSILPAVEDLIGPTRRMMATGGFARSALWRQMMADIFNREVVVPQSVE-SSCL 316 EGV F + A++ GG +RSA W + A + E + L Sbjct: 372 EGVAFAMADGYAALQAAGTTLESAAFVGGGSRSAFWGVLCATALGIPLRRHAGAEVGAAL 431 Query: 317 GAAVLGLFA 325 GAA LG A Sbjct: 432 GAARLGRLA 440 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,184,675 Number of extensions: 48316 Number of successful extensions: 132 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 5 Length of query: 385 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 298 Effective length of database: 1,207,925 Effective search space: 359961650 Effective search space used: 359961650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)