BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0038.0021.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 452 e-128 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 142 4e-35 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 130 9e-32 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 129 2e-31 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 128 4e-31 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 452 bits (1163), Expect = e-128, Method: Compositional matrix adjust. Identities = 220/260 (84%), Positives = 238/260 (91%) Query: 2 TATLPAGLQSFSLSGRLALITGSSGGIGLALARGLAAAGASIVLNGRDAAKLAVVAAKLR 61 T LPAGLQ FSL G+LALITGSSGGIGLALARG+A AGA+IVLNGRDA KLA VAA+LR Sbjct: 5 TTPLPAGLQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLR 64 Query: 62 EEGYTIHESSFDATSSASVEAAVAKIEAGIGPIDILFNNAGIQRRAPLEDFKESDWNDLI 121 EG+TIHESSFDATSS V+AAV KIE+ IGPI IL NNAGIQRR PLE+FKESDW+DLI Sbjct: 65 AEGHTIHESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLI 124 Query: 122 STNLNSVFFTAKAVAQGMIKRGRGKIINVCSVQSELGRPNIAPYTATKGAVKMFTKGMAI 181 STNLNSVFF A+AVA+ MI RG GKIIN+CSVQSELGRP IAPYTATKGAVKMFTKGMAI Sbjct: 125 STNLNSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAI 184 Query: 182 DWGKYGLQVNGLGPGYFKTEMNMALVADEKFSEWLIGRTPSRRWGDVEDLVGAAVFLSSD 241 DWGKYG+QVNGLGPGYFKTEMNMALV D KF++WL+GRTPSRRWGDVEDLVGAAVFL+SD Sbjct: 185 DWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASD 244 Query: 242 ASKFVNGHILYVDGGVTATL 261 AS+FVNGHILYVDGGVTATL Sbjct: 245 ASRFVNGHILYVDGGVTATL 264 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 142 bits (357), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 81/245 (33%), Positives = 132/245 (53%), Gaps = 3/245 (1%) Query: 14 LSGRLALITGSSGGIGLALARGLAAAGASIVLN-GRDAAKLAVVAAKLREEGYTIHESSF 72 +S R+A +TG GGIG A+ L G ++V G ++ + A +RE+G+ IH S Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 Query: 73 DATSSASVEAAVAKIEAGIGPIDILFNNAGIQRRAPLEDFKESDWNDLISTNLNSVFFTA 132 + ++ S +AA K+ A IG +D+L NNAGI R + DW+ +I TNLNS+F Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 Query: 133 KAVAQGMIKRGRGKIINVCSVQSELGRPNIAPYTATKGAVKMFTKGMAIDWGKYGLQVNG 192 K V +GM +RG G+IIN+ SV + G+ Y+ K + FT +A + G+ VN Sbjct: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 Query: 193 LGPGYFKTEMNMALVADEKFSEWLIGRTPSRRWGDVEDLVGAAVFLSSDASKFVNGHILY 252 + PGY T+M A+ + E ++ P +R G+ E++ +++S + G Sbjct: 181 VSPGYIGTDMVRAIRPE--VLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFS 238 Query: 253 VDGGV 257 ++GG+ Sbjct: 239 LNGGL 243 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 130 bits (328), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 75/243 (30%), Positives = 125/243 (51%), Gaps = 1/243 (0%) Query: 17 RLALITGSSGGIGLALARGLAAAGASIVLNGRDAAKLAVVAAKLREEGYTIHESSFDATS 76 R+AL+TG + GIG ++++ LAA G ++++ + + A L G+ D S Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 77 SASVEAAVAKIEAGIGPIDILFNNAGIQRRAPLEDFKESDWNDLISTNLNSVFFTAKAVA 136 S+ AA A IE G D+L NNAG+ R D+ +W ++ N+ V + A Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 137 QGMIKRGRGKIINVCSVQSELGRPNIAPYTATKGAVKMFTKGMAIDWGKYGLQVNGLGPG 196 + M+K+ G+I+N+ S+ Y +K AV T+ MAI+ +YG+ VN + PG Sbjct: 129 RLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPG 188 Query: 197 YFKTEMNMALVADEKFSEWLIGRTPSRRWGDVEDLVGAAVFLSSDASKFVNGHILYVDGG 256 T M A+ E+ E P RR+G +++ A +L+++ S ++ GH++ VDGG Sbjct: 189 PVDTPMTQAM-HSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 Query: 257 VTA 259 A Sbjct: 248 FVA 250 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 129 bits (324), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 88/249 (35%), Positives = 123/249 (49%), Gaps = 6/249 (2%) Query: 13 SLSGRLALITGSSGGIGLALARGLAAAGASIVLNGRDAAKLAVVAAKLREEGYTIHESSF 72 +L G++ALITG+S GIG A A A GA+++L R L VAA + +G + Sbjct: 3 TLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAG 62 Query: 73 DATSSASVEAAVAKIEAGIGPIDILFNNAG-IQRRAPLEDFKESDWNDLISTNLNSVFFT 131 D +A+ E VA G +DI NNAG + PL + W D + NL S F Sbjct: 63 DVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLG 122 Query: 132 AKAVAQGMIKRGRGKIINVCS-VQSELGRPNIAPYTATKGAVKMFTKGMAIDWGKYGLQV 190 A+ M+ RG G I+ S V S G P +A Y A K + KG+A D+ G++ Sbjct: 123 ARCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRA 182 Query: 191 NGLGPGYFKTEMNMALVADEKFSEWLIGRTPSRRWGDVEDLVGAAVFLSSDASKFVNGHI 250 N L PG T+M D + +W G +R E++ AA+FL+S + FV G Sbjct: 183 NALLPGGVDTDMG----GDPQQKQWAAGLHAMKRIAQPEEIAAAALFLASPMASFVTGTA 238 Query: 251 LYVDGGVTA 259 LY DGG +A Sbjct: 239 LYADGGNSA 247 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 128 bits (322), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 85/260 (32%), Positives = 134/260 (51%), Gaps = 5/260 (1%) Query: 1 MTATLPAGLQSFSLSGRLALITGSSGGIGLALARGLAAAGASIVLNGRDAAKLAVVAAKL 60 M+A P+ LQ F G++ ++TG++ GIG A AR + GA ++L RDAA L V L Sbjct: 1 MSALNPSRLQRFE--GKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSL 58 Query: 61 REEGYTIHESSFDATSSASVEAAVAKIEAGIGPIDILFNNAGIQRRAPLEDFKESDWNDL 120 E E+ D + V V G +D+L ++AG+ + + DW+ + Sbjct: 59 PPERTAARET--DVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRV 116 Query: 121 ISTNLNSVFFTAKAVAQGMIKRGRGKIINVCSVQSELGRPNIAPYTATKGAVKMFTKGMA 180 +TN+N VF+ A+ A +++ RG I+NV SV N++ Y A+KGAV T+ MA Sbjct: 117 QATNVNGVFYGARE-ALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMA 175 Query: 181 IDWGKYGLQVNGLGPGYFKTEMNMALVADEKFSEWLIGRTPSRRWGDVEDLVGAAVFLSS 240 +D+G+ G+++N + P T M + D + R R D ++ FL+S Sbjct: 176 LDFGRKGVRINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLAS 235 Query: 241 DASKFVNGHILYVDGGVTAT 260 + FVNG L VDGG+ A+ Sbjct: 236 PDASFVNGVNLPVDGGLMAS 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 630,759 Number of extensions: 22229 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 50 Number of HSP's gapped: 111 Number of HSP's successfully gapped: 50 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)