BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0038.0020.p (268 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 365 e-102 gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 115 3e-27 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 73 2e-14 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 66 3e-12 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 60 2e-10 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 365 bits (937), Expect = e-102, Method: Compositional matrix adjust. Identities = 174/202 (86%), Positives = 185/202 (91%) Query: 1 MQQGIVIHAPHDLRVQELQTAELQPHQLKVKVKAGGICGSDLHYYHNGGFGTVRIKEPMV 60 MQQG+VIHAPHDLR+Q L T LQP QLKVKVKAGGICGSDLHYYH+GGFG VRIKEPMV Sbjct: 1 MQQGLVIHAPHDLRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMV 60 Query: 61 LGHEVSGVIAETGAAISDIKAGTRISISPSNPCGLCKYCQEGKHNHCLDMRFYGSAMRMP 120 LGHEVSG IAE G A++DI GTRI+ISPS PCG CKYCQEGK NHCLDMRFYGSAMR P Sbjct: 61 LGHEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTP 120 Query: 121 HVQGAFRQEIIIERAQAHIVADHVSDGEAAMAEPLSVALHAVRRAGPLFGKRVLVTGCGP 180 HVQGAFRQEIIIER+QAH+VADHVSDGEAAMAEPLSVALHAVRRAGPL GKRVLVTGCGP Sbjct: 121 HVQGAFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGP 180 Query: 181 IGALTVIAARRAGALQIVVTDV 202 IGAL V+AARRAGAL++VVTDV Sbjct: 181 IGALVVVAARRAGALEVVVTDV 202 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 115 bits (289), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 68/202 (33%), Positives = 112/202 (55%), Gaps = 5/202 (2%) Query: 2 QQGIVIHAPHDLRVQELQTAE-LQPHQLKVKVKAGGICGSDLHYYHNGGFGTVRIKEPMV 60 Q +V+ A +L+++E+ + + +++++ GICGSDLHYY +G G +++ PMV Sbjct: 1 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 Query: 61 LGHEVSGVIAETGAAISDIKAGTRISISPSNPCGLCKYCQEGKHNHCLDMRFYGSAMRMP 120 LGHE SG + E G+A+S +K G R+ + P P G +N +RF+ + P Sbjct: 61 LGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWAT----P 116 Query: 121 HVQGAFRQEIIIERAQAHIVADHVSDGEAAMAEPLSVALHAVRRAGPLFGKRVLVTGCGP 180 + G ++ A + + D+VS E A+ EPLS+ L A +A G +V G G Sbjct: 117 PIHGCLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGT 176 Query: 181 IGALTVIAARRAGALQIVVTDV 202 IGA+T +AA GA ++++ DV Sbjct: 177 IGAMTALAALAGGAARVILADV 198 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 73.2 bits (178), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 61/218 (27%), Positives = 94/218 (43%), Gaps = 24/218 (11%) Query: 5 IVIHAPHDLRVQELQTAELQP-HQLKVKVKAGGICGSDLHYYHNGGFGTVRIKEPMVLGH 63 ++ H+ HD+RV+ + +Q + +KV A ICGSDLH Y T K +LGH Sbjct: 4 LLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRGKIPAT---KAGDILGH 60 Query: 64 EVSGVIAETGAAISDIKAGTRISISPSNPCGLCKYCQEGKHNHCLDMR------------ 111 E GV+ E G AI +K G R+ + CG C +C + + C Sbjct: 61 EFMGVVVEAGPAIKRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGALMNAKGI 120 Query: 112 FYGSAMR--------MPHVQGAFRQEIIIERAQAHIVADHVSDGEAAMAEPLSVALHAVR 163 G+AM MP Q + + + I + + +++ L AV+ Sbjct: 121 RSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK 180 Query: 164 RAGPLFGKRVLVTGCGPIGALTVIAARRAGALQIVVTD 201 AG G V + G GP+G + +AR GA +I + D Sbjct: 181 NAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVD 218 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 52/206 (25%), Positives = 95/206 (46%), Gaps = 10/206 (4%) Query: 1 MQQGIVIHAPHDLRVQELQTAELQPHQLKVKVKAGGICGSDLHYYHNGGFGTVRIKEPMV 60 M+ +V L ++++ P Q+ VK +A G+C +DLH H G V+ P + Sbjct: 5 MKAAVVREFGKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAH--GDWPVKPTPPFI 62 Query: 61 LGHEVSGVIAETGAAISDIKAGTRISISP-SNPCGLCKYCQEGKHNHCLDMRFYGSAMRM 119 GHE +G +A GA + +K G R+ + CG C C+ G C + + G + Sbjct: 63 PGHEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYS--- 119 Query: 120 PHVQGAFRQEIIIERAQAHIVADHVSDGEAAMAEPLSVALH-AVRRAGPLFGKRVLVTGC 178 V G+F + + + + D++ G AA V ++ ++ G+ V+++G Sbjct: 120 --VNGSFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGEWVVISGI 177 Query: 179 GPIGALTVIAARRAGALQIVVTDVQR 204 G +G + V A+ G + +V D+ Sbjct: 178 GGLGHMAVQYAKAMG-MHVVAADIHE 202 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 59.7 bits (143), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 33/96 (34%), Positives = 51/96 (53%), Gaps = 6/96 (6%) Query: 13 LRVQELQTAELQPHQLKVKVKAGGICGSDLHYYHNGGFGTVRIKE-PMVLGHEVSGVIAE 71 L+++EL P ++ V++KA G+C SDL VR + PM LGHE S + E Sbjct: 23 LKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITG-----VRPRPVPMALGHEASAEVVE 77 Query: 72 TGAAISDIKAGTRISISPSNPCGLCKYCQEGKHNHC 107 G+ ++D++AG + + CG C C EG+ C Sbjct: 78 VGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALC 113 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 741,203 Number of extensions: 28105 Number of successful extensions: 113 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 7 Length of query: 268 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 184 Effective length of database: 1,222,130 Effective search space: 224871920 Effective search space used: 224871920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)