BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0006.p (190 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) syntheta... 103 8e-24 gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthet... 85 4e-18 >gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) synthetase [Herbaspirillum seropedicae SmR1] Length = 565 Score = 103 bits (257), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 63/156 (40%), Positives = 83/156 (53%), Gaps = 6/156 (3%) Query: 22 VKVAIAQINSTVGDISGNRNKIVEFARRAAAQGADIVLTPELSLVGYPPEDLLLRQAFYA 81 +++AIAQ N T+GD GN ++ +RAA GAD+V+ PELS+ GY P DLL F Sbjct: 2 LRIAIAQCNPTIGDFEGNLALHLDGLQRAAQAGADLVVFPELSICGYYPGDLLEEARFLD 61 Query: 82 KTEEALQALTAELATLKDVHVVVGHPVLKDGAR---FNAASVLLNGRITGSYHKLELPND 138 + E LQ + L + V+G P G FNA + GRI YHK LP Sbjct: 62 RAEATLQDVIDATRRLPGLTAVIGAPRRNPGPGKPLFNALLAVREGRIVAEYHKQLLPVY 121 Query: 139 SVFDEKRYFT--PDGTPLVFDVKGTKFGINICEDTW 172 VFD+ R+F P+ T V +KG + G ICED W Sbjct: 122 GVFDDGRHFEAGPE-TACVLPLKGMRVGFLICEDGW 156 >gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthetase [Herbaspirillum seropedicae SmR1] Length = 685 Score = 85.1 bits (209), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 63/196 (32%), Positives = 95/196 (48%), Gaps = 21/196 (10%) Query: 13 YFLLVAIMSVKVAIAQINSTVGDISGNRNKIVEFARRAAAQGADIVLTPELSLVGYPPED 72 +F L + +VA+A + V D N + + A +AAA+GA +V PEL L Y ED Sbjct: 5 FFNLYSHGFARVAVAVPDCKVADPGFNAQRTIALAEQAAARGAVLVSFPELGLSAYSCED 64 Query: 73 LLLRQAFYAKTEEALQALTAELATLKDVHVVVGHPVLKDGARFNAASVLLNGRITGSYHK 132 L ++A + +ALQ++ E + ++VG P+ D +N A VL +GRI G K Sbjct: 65 LFQQRALLDASLQALQSVL-EASMRIPAALIVGLPLKVDHQLYNCAVVLHHGRILGVVPK 123 Query: 133 LELPNDSVFDEKRYFTPD-----------------GTPLVFDVKGT---KFGINICEDTW 172 LPN S F E R F+ G+ L+F+++ +F I ICED W Sbjct: 124 SYLPNYSEFYEARQFSSADCAVTRSVHLLGQEVGFGSHLLFEIRNIPDFRFHIEICEDVW 183 Query: 173 FPRAPALPXACPAASI 188 P P+ A A++ Sbjct: 184 VPIPPSSFAALAGATV 199 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 463,465 Number of extensions: 16691 Number of successful extensions: 58 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 54 Number of HSP's successfully gapped: 2 Length of query: 190 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 110 Effective length of database: 1,241,070 Effective search space: 136517700 Effective search space used: 136517700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)