BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0001.p (413 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 554 e-159 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 481 e-137 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 398 e-112 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 358 e-100 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 357 1e-99 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 554 bits (1427), Expect = e-159, Method: Compositional matrix adjust. Identities = 265/405 (65%), Positives = 329/405 (81%) Query: 1 VTWRLLPFLFLCYVAAYLDRVNVGFAKLQMLSDLHFSETVYGLGAGIFFIGYFFFEVPSN 60 VTWRLLP LFLCYVA+YLDRVNVGFAKLQML DL FS+TVYGLGAGIFF+GYF FEVPSN Sbjct: 24 VTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFVFEVPSN 83 Query: 61 ILLHRVGARIWIGRIMISWALLSAATLFVTTPTMFYVVRFLLGVAEAGFFPGIVLYLTYW 120 ++LH+VGAR+WI RIMI+W ++S A +VTTP MFY++RFLLGVAEAGFFPG++LYLTYW Sbjct: 84 MILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLTYW 143 Query: 121 FPQRRRSRMNALFMIGIPFAGVVGGPLSGWILQASDGMHGMTGWQWLFLLEAIPSLILGI 180 +P RR ++ ALFM GI +GV+GGPLSGWI+ A G+ G TGWQW+F+LEAIPSLILG+ Sbjct: 144 YPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIPSLILGV 203 Query: 181 ATIIYLPDSIHDARWLSSEEKQLLQENIKVEESSKQAHSAASVFSDGKMWLLAAVYFCCM 240 I+Y+ D I DA WLS EEK+LL+ NI EES K+ S ++F++G++WL A +YFC + Sbjct: 204 MVILYMKDRIRDADWLSEEEKRLLEANISHEESQKEHLSLGAMFANGRVWLGALIYFCFV 263 Query: 241 MGLYGVGFYLPTLIKASGVKNALDVGLLTAIPYGCAIVAMLLNAGSSDRMHERRWHFAIA 300 MGLYGV F+LPT+IKA+GV + L+VGLLTAIPY A AM+L S+D ERRWH AI Sbjct: 264 MGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRERRWHVAIP 323 Query: 301 SVLGGAGLFLSTVYASNVMFAMAALSLGTAGVLATMPVFWTYPSAILSGTAAAAGIAMIN 360 + +G GL LST Y N AMA+L+L + G+L T+P+FW+ P+A LSGTAAAAGIA+IN Sbjct: 324 AFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAAAGIALIN 383 Query: 361 SIGNLAGFVSPSVIGWLKDATQSTNSGMYIVAGALLTGALLILPL 405 S+GNLAGFVSP ++GWLKDATQSTN+GMY++A +L+ G LL L L Sbjct: 384 SLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTL 428 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 481 bits (1237), Expect = e-137, Method: Compositional matrix adjust. Identities = 232/407 (57%), Positives = 312/407 (76%), Gaps = 1/407 (0%) Query: 1 VTWRLLPFLFLCYVAAYLDRVNVGFAKLQMLSDLHFSETVYGLGAGIFFIGYFFFEVPSN 60 V WR++PFL LCYV AYLDRVNVGFAKLQM DL FSETV+GLGAG+FFIGYF FEVPSN Sbjct: 25 VFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEVPSN 84 Query: 61 ILLHRVGARIWIGRIMISWALLSAATLFVTTPTMFYVVRFLLGVAEAGFFPGIVLYLTYW 120 IL+H+VGARIWI RIMI+W +LSAA +FV T FY++RFLLG+AEAGF+PGI+LYLTYW Sbjct: 85 ILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYLTYW 144 Query: 121 FPQRRRSRMNALFMIGIPFAGVVGGPLSGWILQASDGMHGMTGWQWLFLLEAIPSLILGI 180 FP RR+++ A+FM GIP AG++G PLSGWI+ A G+ GWQW+FL+EAIP++++G+ Sbjct: 145 FPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVLIGV 204 Query: 181 ATIIYLPDSIHDARWLSSEEKQLLQENIKVEESSKQA-HSAASVFSDGKMWLLAAVYFCC 239 AT++YL + + A+WL+ EEK LQ +I + K++ H ++ D ++WL+ +YF Sbjct: 205 ATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDARVWLMCLIYFSF 264 Query: 240 MMGLYGVGFYLPTLIKASGVKNALDVGLLTAIPYGCAIVAMLLNAGSSDRMHERRWHFAI 299 +MG YG+ ++PTL+KA+GVK L++GLL+AIP+GCAI+AM L S+DRM ERRWH I Sbjct: 265 VMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRERRWHLVI 324 Query: 300 ASVLGGAGLFLSTVYASNVMFAMAALSLGTAGVLATMPVFWTYPSAILSGTAAAAGIAMI 359 +++GG G + ++A N ++A+LSL AGVL P+FW+ P+A LSG AAA GIA I Sbjct: 325 PALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAAAVGIAAI 384 Query: 360 NSIGNLAGFVSPSVIGWLKDATQSTNSGMYIVAGALLTGALLILPLQ 406 NS+GNLAGFVSP +IG+LKD T + +GMY++A L+ G++ L + Sbjct: 385 NSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLATK 431 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 398 bits (1022), Expect = e-112, Method: Compositional matrix adjust. Identities = 208/404 (51%), Positives = 280/404 (69%), Gaps = 1/404 (0%) Query: 1 VTWRLLPFLFLCYVAAYLDRVNVGFAKLQMLSDLHFSETVYGLGAGIFFIGYFFFEVPSN 60 + WRL+PFL +V A++DRVNVGFAKLQML DL FSE VYGLGAGIFFIGYF FEVPSN Sbjct: 28 IAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFIFEVPSN 87 Query: 61 ILLHRVGARIWIGRIMISWALLSAATLFVTTPTMFYVVRFLLGVAEAGFFPGIVLYLTYW 120 +LL ++GAR + RI I W L S A +VTTPT FY++RFLLG+ EAGFFPG+VLYLTYW Sbjct: 88 LLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVVLYLTYW 147 Query: 121 FPQRRRSRMNALFMIGIPFAGVVGGPLSGWILQASDGMHGMTGWQWLFLLEAIPSLILGI 180 FP +RR+R+N LFM AG VGGP++G I+ +G+ + WQWLF+LE IPS++ G Sbjct: 148 FPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIPSILAGF 207 Query: 181 ATIIYLPDSIHDARWLSSEEKQLLQENIKVEESSKQAH-SAASVFSDGKMWLLAAVYFCC 239 A + +LP+ +A+WLS EK + + + E S+ H S ++ ++W+ AAVYFC Sbjct: 208 AVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNYRVWICAAVYFCV 267 Query: 240 MMGLYGVGFYLPTLIKASGVKNALDVGLLTAIPYGCAIVAMLLNAGSSDRMHERRWHFAI 299 + G + F+ P++IK GVK L +GL++AIP+ +AM+ N SD+ ERR H AI Sbjct: 268 VSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGERRLHSAI 327 Query: 300 ASVLGGAGLFLSTVYASNVMFAMAALSLGTAGVLATMPVFWTYPSAILSGTAAAAGIAMI 359 A+++ GL + + N A+ +L+L G+LA PVFW+ P A L+GTAAA GIA+I Sbjct: 328 ATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAAGGIALI 387 Query: 360 NSIGNLAGFVSPSVIGWLKDATQSTNSGMYIVAGALLTGALLIL 403 NSIGNLAGFV+P +IG LK +T S +SG+Y VA L + L++ Sbjct: 388 NSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVV 431 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 358 bits (918), Expect = e-100, Method: Compositional matrix adjust. Identities = 189/422 (44%), Positives = 274/422 (64%), Gaps = 12/422 (2%) Query: 1 VTWRLLPFLFLCYVAAYLDRVNVGFAKLQMLSDLHFSETVYGLGAGIFFIGYFFFEVPSN 60 VTW++LP+L L YV AYLDRVNVGFAKL MLSDL SE YGLGAGIFF+GYF FEVPSN Sbjct: 19 VTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFFLGYFIFEVPSN 78 Query: 61 ILLHRVGARIWIGRIMISWALLSAATLF---------VTTPTMFYVVRFLLGVAEAGFFP 111 ++LHRVGA+IWI RIMI+W ++S+ F V+ T+FY +RFLLG +EAGFFP Sbjct: 79 LILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYTLRFLLGASEAGFFP 138 Query: 112 GIVLYLTYWFPQRRRSRMNALFMIGIPFAGVVGGPLSGWILQASDGMHGMTGWQWLFLLE 171 G++LY+TYW+P SR+ A+ ++G PF+ ++GGP+SG ++Q + GWQW+F++E Sbjct: 139 GVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGDSLAGWQWMFVIE 198 Query: 172 AIPSLILGIATIIYLPDSIHDARWLSSEEKQLLQENIKVEESSKQAHSAASVFSDGKMWL 231 A+P+ +LGI + L + I A+WL+ EEK L + E K S+++ +W Sbjct: 199 ALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEKVNVPLKSLYALPILWF 258 Query: 232 LAAVYFCCMMGLYGVGFYLPTLIKASGVKNALDVGLLTAIPYGCAIVAMLLNAGSSDRMH 291 LA VY M+G YG+ F+ PT+I+ +GVK+ LD+G+++AIPY AM+ +++ Sbjct: 259 LALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGATAMVFITRHAEKAG 318 Query: 292 ERRWHFAIASVLGGAGLFLSTVYASNVMFAMAALSLGTAGVLATMPVFWTYPSAILSGTA 351 + R + S++ G L L T++ N+ +A +++ G L ++ +FW +P A +G Sbjct: 319 KHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIALFWGFPGAYFTGAT 378 Query: 352 AAAGIAMINSIGNLAGFVSPSVIGWLKDATQSTNSGMYIVAGALLTGALLILPLQQTGKK 411 AAAGIA+INS+GNL GFV P ++G L A S G+ ++ GAL +LI G+K Sbjct: 379 AAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGALFLAGVLI---ALGGRK 435 Query: 412 TG 413 G Sbjct: 436 RG 437 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 357 bits (916), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 192/413 (46%), Positives = 265/413 (64%), Gaps = 6/413 (1%) Query: 1 VTWRLLPFLFLCYVAAYLDRVNVGFAKLQMLSDLHFSETVYGLGAGIFFIGYFFFEVPSN 60 V RL+PF+ LCYV +YLDR+NVGFA L M DL + + +GLGAG+FFIGYFFFE+PSN Sbjct: 27 VMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYFFFEIPSN 86 Query: 61 ILLHRVGARIWIGRIMISWALLSAATLFVTTPTMFYVVRFLLGVAEAGFFPGIVLYLTYW 120 + LHR GAR+WI RIMISW L+S AT FV P F + RFLLG+AEAGF PGI LY T W Sbjct: 87 LALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGIYLYFTQW 146 Query: 121 FPQRRRSRMNALFMIGIPFAGVVGGPLSGWILQASDGMHGMTGWQWLFLLEAIPSLILGI 180 FP R + A F+IGIP A ++G PLSG +++ GM G GWQ L LLEA+P+++LG+ Sbjct: 147 FPGAWRGKATAFFLIGIPVANIIGSPLSGALMEL-HGMWGFKGWQVLLLLEALPAVLLGV 205 Query: 181 ATIIYLPDSIHDARWLSSEEKQLLQENIKVEES---SKQAHSAASVFSDGKMWLLAAVYF 237 + LPD A+WLS++EKQ L+ + E++ ++ + F++ +++ LA F Sbjct: 206 MCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFALAGANF 265 Query: 238 CCMMGLYGVGFYLPTLIKASGVKNALDVGLLTAIPYGCAIVAMLLNAGSSDRMHERRWHF 297 C ++G +G +LP +I+ G+ + VGL+ AIPY VAM L A ++R R + Sbjct: 266 CGIIGSLSIGLWLPQIIREFGLPSH-QVGLVAAIPYLVGAVAMTLWARLANRSERRLFFV 324 Query: 298 AIASVLGGAGLFLSTVYASNVMFAMAALSLGTAGVLATMPVFWTYPSAILSGTAAAAGIA 357 A A VL L +S + + M A+++ A +L+ FW PS L+G AAA G+A Sbjct: 325 AGAIVLAALSLGISA-FLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGRAAAGGLA 383 Query: 358 MINSIGNLAGFVSPSVIGWLKDATQSTNSGMYIVAGALLTGALLILPLQQTGK 410 +I SIGNL GFV PSVIG++++ATQ + VAGALL GA++ L L + Sbjct: 384 LIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLALGDPSR 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,243,390 Number of extensions: 51773 Number of successful extensions: 364 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 326 Number of HSP's successfully gapped: 19 Length of query: 413 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 325 Effective length of database: 1,203,190 Effective search space: 391036750 Effective search space used: 391036750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)