BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0034.0002.p
         (299 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ...   472   e-134
gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm...   126   2e-30
gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra...    84   1e-17

>gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD
           family secretion protein [Herbaspirillum seropedicae
           SmR1]
          Length = 457

 Score =  472 bits (1215), Expect = e-134,   Method: Compositional matrix adjust.
 Identities = 234/293 (79%), Positives = 264/293 (90%), Gaps = 4/293 (1%)

Query: 11  ASFAARQSKLTQELSVLREQSAQRQAEVGEAVSRQKSLVSEFDLAQRQYKLVHGMFERNA 70
           A+FAAR SKL QE+SVLREQSAQRQAE+GEA+SRQKS  SE +LAQRQYKL+H M  RNA
Sbjct: 165 AAFAARSSKLMQEMSVLREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLVRNA 224

Query: 71  ASQLEDIQAQSKAQDAQSRLSATNAMIPRLTAAIAEAQAKMGEAASRFRADARSDLTVSE 130
           ASQLEDIQA S+ QDAQS+L+ATNAMIPRL AAIAEAQAK GEAASRFR+DAR++LT +E
Sbjct: 225 ASQLEDIQAASRVQDAQSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAE 284

Query: 131 NELARLQEEIKSRNDRVSRSEVRAPMDGIVNRVLINTVGGVVKPGDAIIEMTPTDDKLVI 190
            ELARLQEEIKSRNDRVSRSEV+APMDG+VNRV+INT+GGVV+PGD IIEMTP+DDKLVI
Sbjct: 285 TELARLQEEIKSRNDRVSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVI 344

Query: 191 EAKVAPTDRAQLMTGAPARVKVSAYDYGVYGAMKGVVTEVSADTVPEE---RGERFYRVK 247
           EA+++PTDRA+L+TGA ARVKVSAYDYGVYGAM GVVTE+SADTVP+E   RGERFYR+K
Sbjct: 345 EARISPTDRAELVTGARARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLK 404

Query: 248 IEVTR-DPKLDTGLPLMPGMTATADMIVGRRTVWQYLMSPLSRFGQTALREPR 299
           IEV R   +L   L LMPGMTA+AD+IVGRRTVWQYLMSPLSRFGQ ALREPR
Sbjct: 405 IEVKRGHDELQQKLALMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALREPR 457


>gi|300312349|ref|YP_003776441.1| hemolysin-type secretion
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 386

 Score =  126 bits (316), Expect = 2e-30,   Method: Compositional matrix adjust.
 Identities = 71/210 (33%), Positives = 123/210 (58%), Gaps = 9/210 (4%)

Query: 95  AMIPRLTAAIAEAQAKMGEAASRFRADARSDLTVSENELARLQEEIKSRNDRVSRSEVRA 154
           A + RL   +A+ +A++    +++  +A  D+T  + +L   +E+++ R   +  +E++A
Sbjct: 178 ADVLRLQRNVADIRAQINNKRNKYFQEALVDMTKVQEDLNTQREQLEDRTQVLEHTELKA 237

Query: 155 PMDGIVNRVLINTVGGVVKPGDAIIEMTPTDDKLVIEAKVAPTDRAQLMTGAPARVKVSA 214
           P DG+V  + + T+GGVV+ GD ++E+ P D+ L++EAKV+  D A +  G  A VK+ A
Sbjct: 238 PADGVVKNIKVTTIGGVVRSGDVVMEILPVDN-LIVEAKVSTADSAYVKVGQAANVKLDA 296

Query: 215 YDYGVYGAMKGVVTEVSADTVPEERGER---FYRVKI----EVTRDPKLDTGLPLMPGMT 267
           YDY +YG + G V  VS DT+ +E  +    +YRV I     V +  K D  + + PGMT
Sbjct: 297 YDYSIYGTLNGHVVYVSPDTLLDETRQGAMPYYRVHIALEEAVFKGRKAD-AIQVKPGMT 355

Query: 268 ATADMIVGRRTVWQYLMSPLSRFGQTALRE 297
           AT ++    RTV  +L  P+++  Q ++ E
Sbjct: 356 ATVEIKANTRTVLSFLTKPIAKTLQESMGE 385


>gi|300312188|ref|YP_003776280.1| hemolysin secretion-related
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 447

 Score = 84.0 bits (206), Expect = 1e-17,   Method: Compositional matrix adjust.
 Identities = 45/146 (30%), Positives = 77/146 (52%), Gaps = 2/146 (1%)

Query: 154 APMDGIVNRVLINTVGGVVKPGDAIIEMTPTDDKLVIEAKVAPTDRAQLMTGAPARVKVS 213
           AP+ G V ++ ++T+GGVV     ++++ P D ++ +EA +   D   +  G  A VK+ 
Sbjct: 301 APVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDVGFVEIGQAAEVKID 360

Query: 214 AYDYGVYGAMKGVVTEVSADTVPEERGERFYRVKIEVTRDPKLDTG--LPLMPGMTATAD 271
           A+DY  YG +   V  +S D + +E+    Y  +I + +   +  G  + L  GM A  +
Sbjct: 361 AFDYTKYGTLPARVVHISRDAIQDEKKGLLYSARIVLDQSSLIVDGRKVQLSAGMAANVE 420

Query: 272 MIVGRRTVWQYLMSPLSRFGQTALRE 297
           +  G R V +Y +SPL R  + AL E
Sbjct: 421 IKTGNRRVIEYALSPLIRHQKEALHE 446


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.315    0.127    0.340 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 755,927
Number of extensions: 28129
Number of successful extensions: 168
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 164
Number of HSP's successfully gapped: 4
Length of query: 299
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 214
Effective length of database: 1,217,395
Effective search space: 260522530
Effective search space used: 260522530
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 130 (54.7 bits)