BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0033.0009.p
         (200 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309432|ref|YP_003773524.1| glutamate-cysteine ligase [Herb...   357   e-100

>gi|300309432|ref|YP_003773524.1| glutamate-cysteine ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 433

 Score =  357 bits (916), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 169/201 (84%), Positives = 187/201 (93%), Gaps = 1/201 (0%)

Query: 1   MLNPLYAKCGELNLQEDAGSDCLADNVSSLLSKIRKKYKEYGIKEQPFVIVKANAGTYGM 60
           MLNPL+AK  +++ QE  G+D LAD+VSSLLSKIRKKYKEYGIKEQPFV VKANAGTYGM
Sbjct: 233 MLNPLFAKVSDIDFQESTGTDALADSVSSLLSKIRKKYKEYGIKEQPFVFVKANAGTYGM 292

Query: 61  GIMTVRDASEVKDLNRKQRS-KLVVGKEGLEVNELVIQEGVHSFERINEAVAEPVVYMID 119
           GIMTV+DASE+KDLNRKQR+  L  GKEGLEV+E+++QEGVHSFERIN+AVAEPVVYMID
Sbjct: 293 GIMTVKDASEIKDLNRKQRTGMLAAGKEGLEVSEVIVQEGVHSFERINQAVAEPVVYMID 352

Query: 120 RYVVGGFYRVHAERGINENLNAPGAHFVPLAFQQQHALPDLQAKPGTAAPNRFYMYGVVA 179
           RYVVGGFYRVHA+R +NENLNAPGAHFVPLAF QQHALPD+ AKPGTAAPNRFYMYGVVA
Sbjct: 353 RYVVGGFYRVHADRDVNENLNAPGAHFVPLAFAQQHALPDMHAKPGTAAPNRFYMYGVVA 412

Query: 180 RLALLAASLEMEKTDPNPEVY 200
           RLALLAAS+EMEKTDPNPEVY
Sbjct: 413 RLALLAASVEMEKTDPNPEVY 433


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.317    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 574,519
Number of extensions: 21375
Number of successful extensions: 51
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 50
Number of HSP's successfully gapped: 1
Length of query: 200
Length of database: 1,619,870
Length adjustment: 81
Effective length of query: 119
Effective length of database: 1,236,335
Effective search space: 147123865
Effective search space used: 147123865
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 128 (53.9 bits)