BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0032.0019.p
         (247 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbas...   467   e-133
gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac...    58   6e-10
gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cyc...    58   7e-10

>gi|300310362|ref|YP_003774454.1| galactonate dehydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 382

 Score =  467 bits (1202), Expect = e-133,   Method: Compositional matrix adjust.
 Identities = 224/245 (91%), Positives = 233/245 (95%)

Query: 3   RGFTAVKMNGTEEMQFIDTWDKVELTLANVAAVREAVGPNVGIGVDFHGRVHKPMAKVLV 62
           RGFTAVKMNGTEE+QFID+ DK+ELTLANV AVREAVGPNVGIGVDFHGRVHKPMAK L+
Sbjct: 138 RGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGIGVDFHGRVHKPMAKALI 197

Query: 63  KELEQYKLMFIEEPVLSENYEALKEIAHLTGTPIALGERLYSRWDFKRILSEGYVDIIQP 122
           KELE YKLMFIEEPVLSEN EALKEIAHLT TPIALGERLYSRWDFKRILSEGYVDIIQP
Sbjct: 198 KELEPYKLMFIEEPVLSENAEALKEIAHLTSTPIALGERLYSRWDFKRILSEGYVDIIQP 257

Query: 123 DVSHAGGITETRKIATMAEAYDVALALHCPLGPIALAACLQVDAVSYNAFIQEQSLGIHY 182
           DVSHAGGITETRKIA MAEAYDVALALHCPLGPIALA+CLQVDA SYNAFIQEQSLGIHY
Sbjct: 258 DVSHAGGITETRKIAMMAEAYDVALALHCPLGPIALASCLQVDAGSYNAFIQEQSLGIHY 317

Query: 183 NESNDLLDYVTDADVFSYKDGYVTIPNGPGLGIEINEEYVRQRAEVGHRWRNPIWRHKDG 242
           NESNDLLDY+ D +VF+Y+DGYV IP GPGLGIEINEEYVRQRAEVGHRWRNPIWRHKDG
Sbjct: 318 NESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVRQRAEVGHRWRNPIWRHKDG 377

Query: 243 SFAEW 247
           SFAEW
Sbjct: 378 SFAEW 382


>gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate
           lactonizing [Herbaspirillum seropedicae SmR1]
          Length = 424

 Score = 58.2 bits (139), Expect = 6e-10,   Method: Compositional matrix adjust.
 Identities = 62/254 (24%), Positives = 117/254 (46%), Gaps = 26/254 (10%)

Query: 1   VARGFTAVKMNGTEEMQFIDTWDKVELTLANVAAVREAVGPNVGIGVDFHGRVHKPMAKV 60
           VA+    ++ NG + ++        E  +A + A+R+A  P+  + +D +G      A +
Sbjct: 175 VAQARKMIEENGFQSIKLKAGALAPEHEVACIKALRKAF-PDAPLRIDPNGNWSLQTA-I 232

Query: 61  LVKELEQYKLMFIEEPVLSENYEALKEIAHLTGTPIALGERLYSRWDFKRILSEGYVDII 120
            + EL    L + E+P  +   E + E+   TG P+A    +    + +R ++   V I+
Sbjct: 233 RMAELLGDDLQYYEDP--TPGLEGMAELHRRTGLPLATNMVVTDFDELRRSVALDSVQIV 290

Query: 121 QPDVSHAGGITETRKIATMAEAYDVALALHCP--LGPIALAACLQVDAVSYNAFIQEQSL 178
             D  + GG+ +T+++A M + + + +++H    LG I+L A   V A   N      + 
Sbjct: 291 LADHHYWGGLRDTQQLAKMCDVFGLGVSMHSNSHLG-ISLMAMSHVAAAVENL---SYAC 346

Query: 179 GIHYN-ESNDLLDYVTDADVFSYKDGYVTIPNGPGLGIEINEEY------------VRQR 225
             HY  +  D  + V        ++G V I + PGLG+E++++             +RQR
Sbjct: 347 DTHYPWQEPD--EEVIQGGKLPIRNGCVQITDAPGLGVEVDQDQLRKLHQLYLECGIRQR 404

Query: 226 AEVGH-RWRNPIWR 238
            +VG  R   P W+
Sbjct: 405 DDVGQMRKYQPDWK 418


>gi|300312959|ref|YP_003777051.1| muconate and chloromuconate
           cycloisomerase [Herbaspirillum seropedicae SmR1]
          Length = 386

 Score = 57.8 bits (138), Expect = 7e-10,   Method: Compositional matrix adjust.
 Identities = 48/199 (24%), Positives = 92/199 (46%), Gaps = 7/199 (3%)

Query: 25  VELTLANVAAVREAVGPNVGIGVDFHGRVHKPMAKVLVKELEQYKLMFIEEPVLSENYEA 84
           V+   A+VAA++ A+G    + VD +    +  A + ++ L    +  +E+P+ + N++ 
Sbjct: 179 VQQDCAHVAAIKRALGERGSVRVDVNQAWTETEAMLGMQWLADAGVDLVEQPIAASNHDG 238

Query: 85  LKEIAHLTGTPIALGERLYSRWDFKRILSEGYVDIIQPDVSHAGGITETRKIATMAEAYD 144
           L+ +       I   E L+   D  ++   G  D+    ++ +GG+   +++A +A A  
Sbjct: 239 LRRLRQKNRIAIMADESLHGPQDAYQLARAGAADVFAVKINQSGGLAGAQQVAAIASAAQ 298

Query: 145 VALALHCPL-GPIALAACLQVDAVSYNAFIQEQSLGIHYNESNDLLDYVTDADVFSYKDG 203
           V L     L G I   A  Q+ A ++        L      + ++L     A    Y+D 
Sbjct: 299 VELYGGTMLEGAIGTMASAQLFA-TFGTLAWGSELFGPLLLTEEIL-----ATPLRYRDF 352

Query: 204 YVTIPNGPGLGIEINEEYV 222
            + +P GPGLGI ++E+ V
Sbjct: 353 ALELPTGPGLGITLDEDRV 371


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.137    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 804,689
Number of extensions: 33848
Number of successful extensions: 85
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 85
Number of HSP's successfully gapped: 3
Length of query: 247
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 164
Effective length of database: 1,226,865
Effective search space: 201205860
Effective search space used: 201205860
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 129 (54.3 bits)