BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0017.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 509 e-145 gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 279 3e-76 gi|300313718|ref|YP_003777810.1| cobalt-zinc-cadmium resistance ... 97 2e-21 gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 87 3e-18 gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 65 8e-12 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 509 bits (1311), Expect = e-145, Method: Compositional matrix adjust. Identities = 262/348 (75%), Positives = 293/348 (84%), Gaps = 30/348 (8%) Query: 1 MHWPLFALAVAAFGIGTTEFVIMGLLPDVARDLGVSAPAAGMLVSGYALGVAAGAPVLAI 60 M+WPLFALAVAAFGIGTTEFVIMGLLPDVARDLGV+APAAGMLVSGYALGVAAGAP+LAI Sbjct: 1 MNWPLFALAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAI 60 Query: 61 VTAKWQRKRALVGLMCLFILGNVLCAIAPSYNFLMVARVVTAFCHAAFFGIGSVVAAELV 120 +TAKW RK+ALVGLMCLFILGNVLCA+AP+Y FLMVARVVTAFCHAAFFGIGSVVAAELV Sbjct: 61 LTAKWPRKQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELV 120 Query: 121 PREKRAQAVALMFTGL------------------------------GLAAVIMLWICLPK 150 +KRAQAVALMFTGL G+ A IMLW LPK Sbjct: 121 APQKRAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPK 180 Query: 151 KIAMQGGSIFKEFRAIVHPQVQLALLTSTLASVSMFSVLTYIAYILLDVTGFTAHQESWV 210 KI MQ G+IF+EFR+I+ +VQL+LLTSTLAS+SMFSV+TYIAYILLDVTGFT QESWV Sbjct: 181 KIPMQAGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFTTQQESWV 240 Query: 211 LLLFGAGITVGGLIGGRLADWRLMPVLFSMFVCIALILVQFSWSSHHQIPTLMTMFVWGA 270 LLLFGAGITVGGL+GG+LADW+LMPV+ +F+CIA++L FS +SH++ PTL TMF WGA Sbjct: 241 LLLFGAGITVGGLVGGKLADWKLMPVMCGVFLCIAVVLGLFSITSHYKWPTLATMFAWGA 300 Query: 271 FSFAVVPAAQIRVLDKARDAPNLASTLNQGAFNVGNATGAWLGGLAIS 318 F+FAVVP AQIRVLD AR APNLASTLNQGAFN+GNATGAW+GGLAIS Sbjct: 301 FAFAVVPGAQIRVLDTARGAPNLASTLNQGAFNLGNATGAWVGGLAIS 348 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 279 bits (713), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 166/363 (45%), Positives = 222/363 (61%), Gaps = 39/363 (10%) Query: 5 LFALAVAAFGIGTTEFVIMGLLPDVARDLGVSAPAAGMLVSGYALGVAAGAPVLAIVTAK 64 L+AL ++AF IGTTEFVIMGLLP+VA +LGVS +AG+LV+GYALGV GAP+L T + Sbjct: 5 LWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTATTGR 64 Query: 65 WQRKRALVGLMCLFILGNVLCAIAPSYNFLMVARVVTAFCHAAFFGIGSVVAAELVPREK 124 RK LV LM +F++GN+ CAIAP Y LM+ RVV +F H AFFG+GSVVA LVP+EK Sbjct: 65 LPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLVPKEK 124 Query: 125 RAQAVALMFTGLGLAAVI----------------MLW--------------ICLPKKIAM 154 +A A+ALMFTGL +A V+ W + +P+ Sbjct: 125 QASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGG 184 Query: 155 QGGSIFKEFRAIVHPQVQLALLTSTLASVSMFSVLTYIAYILLDVTGFTAHQESWVLLLF 214 + KE R ++ PQV L LL + L +F+ TYIA IL D++GF A S +LLLF Sbjct: 185 EETDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPILLLF 244 Query: 215 GAGITVGGLIGGRLADWRLMPVLFSMFVCIALILVQFSWSSHHQIPTLMTMFVWGAFSFA 274 G G+ +G L GG+LAD +LM L V +A +LV F + +++ ++ + + G +FA Sbjct: 245 GLGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAIIGVGLMGMAAFA 304 Query: 275 VVPAAQIRVLDKARDAPNLASTLNQGAFNVGNATGAWLGGLAISXHAVALALDNNPELSA 334 VP Q+RVL+KA APNLAS+ N AFN+GNA GAWLGG+ I D+ P L A Sbjct: 305 TVPPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTI---------DHGPGLHA 355 Query: 335 ARH 337 + Sbjct: 356 VPY 358 >gi|300313718|ref|YP_003777810.1| cobalt-zinc-cadmium resistance cation efflux system outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 97.1 bits (240), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 46/64 (71%), Positives = 54/64 (84%) Query: 321 AVALALDNNPELSAARHELEAVDATVAQAGARPNPELSTLMEDTRKSTRTTTIQLNQPIE 380 A++LAL+ N L+AAR E+EAV+AT+ QAGARPNPE S LMED RKS+RTTT QL QPIE Sbjct: 51 AISLALEQNKTLAAARKEIEAVEATIIQAGARPNPEFSALMEDFRKSSRTTTYQLTQPIE 110 Query: 381 LGGK 384 LGGK Sbjct: 111 LGGK 114 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 86.7 bits (213), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 94/354 (26%), Positives = 156/354 (44%), Gaps = 59/354 (16%) Query: 8 LAVAAFGIGTTEFVIMGLLPDVARDLGVSAPAAGMLVSGYALGVAAGAPVLAIVTAKWQR 67 LA+ F IGT F+I LLP +A D G+S P LV + L +A +P+ +++A R Sbjct: 29 LALGTFAIGTEGFMIAPLLPRMAADFGMSVPMVASLVVIFTLVLALSSPISTVLSAAMNR 88 Query: 68 KRALVGLMCLFILGNVLCAIAPSYNFLMVARVVTAFCHAAFFGIGSVVAAELVPREKRAQ 127 +R L+ M F N+L A + S+ L+VAR++ A + S +A +V +R + Sbjct: 89 RRVLIAAMTAFTAANLLAACSSSFTGLLVARLLMAVAAGLYTPNASALAGAMVAPGQRGR 148 Query: 128 AVALMFTG------------------------------LGLAAVIMLWICLPKKIA--MQ 155 A+A++ G +G A+ + + +P++ + Sbjct: 149 ALAIVSGGMTSPSALGLPLGAVVGHAFGWRATFLAVSAMGAVALAGILLGVPRQAGAHLH 208 Query: 156 GGSIFKEFRAIVHPQVQLALLTSTLASVSMFSVLTYIAYILLDVTGFTAHQESWVLLLFG 215 + + I PQVQ L + L SV ++ YIA L V F + S + L+G Sbjct: 209 VAGLRERMAVIGQPQVQRLLAVTLLWSVGAYAAYPYIAPYLSHVLSFGESRISLTVSLWG 268 Query: 216 A----GITVGGLIGGRLADWRLMPVLFSMFVCIALILVQFS-WSSHHQI---------PT 261 A G++VGG + R R+ V ++L+L+ S W+ P Sbjct: 269 AAAAVGVSVGGTLNDRYGSARV--------VRVSLLLLMLSFWTLAAATALPPPLALGPV 320 Query: 262 LMTMFVWGAFSFAVVPAAQIRVLDKARDA-PNLASTLNQG----AFNVGNATGA 310 L+ + VWG +A PA R++ A +A +LN F++G+A GA Sbjct: 321 LLAVVVWGFSVWAFFPAQMARLIGAGAPAQAPVALSLNTSTLYLGFSLGSAAGA 374 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 65.5 bits (158), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 48/135 (35%), Positives = 76/135 (56%) Query: 7 ALAVAAFGIGTTEFVIMGLLPDVARDLGVSAPAAGMLVSGYALGVAAGAPVLAIVTAKWQ 66 +LA+ F + T EF+ LL +A DL VS AAG V+ A+ A AP + ++T Sbjct: 41 SLALGVFSLVTAEFLPASLLTAIASDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNID 100 Query: 67 RKRALVGLMCLFILGNVLCAIAPSYNFLMVARVVTAFCHAAFFGIGSVVAAELVPREKRA 126 RKR ++ L L ++ N+L AP+ L+VARV+ F+ + + +A LVP A Sbjct: 101 RKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFA 160 Query: 127 QAVALMFTGLGLAAV 141 +A++L+ TG+ +A V Sbjct: 161 RAMSLILTGVSVATV 175 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,086,603 Number of extensions: 40368 Number of successful extensions: 301 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 303 Number of HSP's successfully gapped: 8 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)