BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0014.p (158 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313718|ref|YP_003777810.1| cobalt-zinc-cadmium resistance ... 195 2e-51 >gi|300313718|ref|YP_003777810.1| cobalt-zinc-cadmium resistance cation efflux system outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 195 bits (495), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 92/157 (58%), Positives = 122/157 (77%) Query: 1 LSKIESSKRIPDATLSLGRKRDEEAGRSMWVVGVSMPLPFLDSNRGNLEEALRRTDKARD 60 L++IE+SKR PD TLS G KRDE R+MWVVG S+P+P LDSN+GN EAL+R D ARD Sbjct: 270 LARIETSKRTPDVTLSFGNKRDESNARNMWVVGFSVPIPVLDSNKGNELEALKRVDIARD 329 Query: 61 ELAAAETRMRTDIAQAYEKLRNAHAEVAVLHSDILPGAQSAYAAAGKGFELGKFNFLEVL 120 EL+ E +M+++ AQ++E+LRNA E L ++I+PGAQSAY AA GFELGKF +L+VL Sbjct: 330 ELSIVEVQMQSEAAQSFERLRNAREEAVALRNEIVPGAQSAYQAARTGFELGKFAYLDVL 389 Query: 121 DAQRTLFQARSQYLRAISDAHLASAELERLLGSAGPN 157 DAQRT FQA++QY +A+S A A+A+L+RL G+ P+ Sbjct: 390 DAQRTYFQAKAQYWKALSTAFQAAADLDRLAGNETPS 426 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 478,172 Number of extensions: 18285 Number of successful extensions: 79 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 78 Number of HSP's successfully gapped: 1 Length of query: 158 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 80 Effective length of database: 1,250,540 Effective search space: 100043200 Effective search space used: 100043200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)