BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0032.0013.p
         (388 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313717|ref|YP_003777809.1| cobalt-zinc-cadmium resistance ...   423   e-119
gi|300313899|ref|YP_003777991.1| RND family efflux transporter M...    64   2e-11

>gi|300313717|ref|YP_003777809.1| cobalt-zinc-cadmium resistance
           cation efflux system transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 396

 Score =  423 bits (1087), Expect = e-119,   Method: Compositional matrix adjust.
 Identities = 219/395 (55%), Positives = 275/395 (69%), Gaps = 43/395 (10%)

Query: 33  PADAHAESGHNHAAEKTTKDDHKEAKGG-HDEDKDHKDDDHDEKGHDGKGHDEDSKIAFS 91
           PA A     H         + H E KG  H +  +H + +H    HD +   E+ KIA +
Sbjct: 6   PAGAEKSPAHQEEGGHKDNEHHGEEKGSEHKDAAEHGEGEH----HDAEPSSEEGKIALN 61

Query: 92  DQQLKTAGIVLDTAAPGRIRTSLQLPGEIRFNQDRTAHVVPKVNGVVESVSANLGQQVKK 151
            +Q+ TAGI + TA P  + +S+QLPGEIRFN+DRTAHVVP+V G+VESVSA LGQ+V+K
Sbjct: 62  PEQIATAGIEVKTAGPMILNSSVQLPGEIRFNEDRTAHVVPQVAGIVESVSAKLGQKVRK 121

Query: 152 GQVLAIVSSSTLSEQRSELLNAQQRLTLAQATHARX------------------------ 187
           GQVLA+++SS +SEQRSE+LNA+QRL LA+  + R                         
Sbjct: 122 GQVLAVINSSAVSEQRSEMLNAEQRLALARTAYQREKQLWEQKISAEQDYLQAQAALREA 181

Query: 188 --------------GAGASASGLNRYEIRAPFDGIVVEKHLALGEAVKEDASIFTISDLS 233
                         GA AS+ GLNRYEIRAPFDG+VVEKHL LGEAVKEDA+ F ISDLS
Sbjct: 182 EVAVRNAQQKLRAIGATASSGGLNRYEIRAPFDGVVVEKHLGLGEAVKEDANAFLISDLS 241

Query: 234 SVWAEMTVAARDMNAVRVGESVTVNATAFASSANGKIAYVGALLGEQTRTAKAHVVLPNP 293
           SVWAE+ V+ +DM  VR GE VTV ATAF +SA+GKI+YVG+L+GEQ+RTAKAHV+LPNP
Sbjct: 242 SVWAEIIVSPKDMQFVRTGEKVTVRATAFDASASGKISYVGSLIGEQSRTAKAHVILPNP 301

Query: 294 GNAWRPGLFVNVDVLAQETDVALAVAADAVQSVEQKQVVFVRVADGFRKQEVTPGRSDGK 353
            ++WRPGLFVNV+V++ E  V +AV+A+A+Q+VE+K V+FVRV  GF  Q+V  GRS+GK
Sbjct: 302 DDSWRPGLFVNVEVVSDEAQVPVAVSAEALQTVEEKSVIFVRVPGGFAAQQVVTGRSNGK 361

Query: 354 RVEILKGLSAGDVYAAAGSFVIKAELGKGSAEHAH 388
            VEI  GL+AGD YAA GSF IKAELGKGSAEH+H
Sbjct: 362 FVEIRSGLNAGDEYAATGSFAIKAELGKGSAEHSH 396


>gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP
           subunit [Herbaspirillum seropedicae SmR1]
          Length = 406

 Score = 64.3 bits (155), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 72/291 (24%), Positives = 132/291 (45%), Gaps = 46/291 (15%)

Query: 113 SLQLPGEIR-FNQDRTAHVVPKVNGVVESVSANLGQQVKKGQVLAIVSSSTLSEQRSELL 171
           +L LPG ++ F Q   + +  +  G ++  + ++G +VKKG++LA + +  + +Q ++ L
Sbjct: 83  TLALPGTLQGFVQ---SPISARSGGYLKRWTKDIGAEVKKGELLAELDTPEIDQQLTQAL 139

Query: 172 NAQQR----LTLAQATHARXGAGASASGLNRYE--------------------------- 200
            A+++    + LA+++ AR  A      +++ E                           
Sbjct: 140 AAREQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASANLAAADANVGRLRQ 199

Query: 201 ------IRAPFDGIVVEKHLALGEAVKEDA--SIFTISDLSSVWAEMTVAARDMNAVRVG 252
                 I APF GI+  +++ +G+ +   A  ++F +S    +   + V     N V+ G
Sbjct: 200 VEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNVPQSYANLVKPG 259

Query: 253 ESVTVNATAF-ASSANGKIAYVGALLGEQTRTAKAHVVLPNPGNAWRPGLFVNVDVLAQE 311
           + VTV  +        GK+A     +   TR+ +  V L N  +A  PG FV V  L  +
Sbjct: 260 QQVTVTQSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSNADHALLPGAFVQV-ALPIK 318

Query: 312 TDVALAVAADAVQSVEQKQVVF-VRVADGFRKQEVTPGRSDGKRVEILKGL 361
              AL VAAD +       +V  V   +  R +++  GR+ G+ VE+L GL
Sbjct: 319 PSGALLVAADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFGQTVEVLDGL 369


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.312    0.127    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 918,915
Number of extensions: 30201
Number of successful extensions: 152
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 152
Number of HSP's successfully gapped: 2
Length of query: 388
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 301
Effective length of database: 1,207,925
Effective search space: 363585425
Effective search space used: 363585425
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 131 (55.1 bits)