BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0012.p (718 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 819 0.0 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 115 9e-27 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 103 3e-23 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 103 6e-23 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 99 1e-21 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 819 bits (2116), Expect = 0.0, Method: Compositional matrix adjust. Identities = 418/612 (68%), Positives = 478/612 (78%), Gaps = 69/612 (11%) Query: 1 MFERIIRFAIEHRWLVLLAVFGLAAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLE 60 MFERIIRFAIEHRWLVLLAV G+ AYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLE Sbjct: 1 MFERIIRFAIEHRWLVLLAVLGMGAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLE 60 Query: 61 TEQRISFPIETVMSGLPNLDQTRSLSRYGLSQVTVIFKDGTDIYFARQLVNERIQEARSK 120 EQRIS+PIET+MSGLPNL+Q RSLSRYGLSQVTVIFKDGTDIYFARQLVNERIQ ARS+ Sbjct: 61 AEQRISYPIETLMSGLPNLEQVRSLSRYGLSQVTVIFKDGTDIYFARQLVNERIQLARSQ 120 Query: 121 LPSGIDPAMGPISTGLGEIYLWTVEAKEGAKNADGVPYSPADLREIQDWIIKPQLRNVPG 180 LP GIDP MGPISTGLGEIYLWTVE+++ A+ DG PY+ DLREIQDW+IKPQLR VPG Sbjct: 121 LPPGIDPGMGPISTGLGEIYLWTVESRDDARKPDGTPYTATDLREIQDWVIKPQLRLVPG 180 Query: 181 VTEINSIGGYAKEYHVAPSPAKLASYGLTLQNVAAALERNNANVGAGYIEKRGEQYLIRA 240 VTEIN+IGG+AKEY VAPS KLASYG+T+ + ALE+NN+NVGAGYIE+RGEQYLIRA Sbjct: 181 VTEINTIGGFAKEYLVAPSAEKLASYGMTMDTIIKALEKNNSNVGAGYIERRGEQYLIRA 240 Query: 241 PGQVASLDDIRNIILGNVQGVAIRIRDVAEVGIGRELRTGAATENGREVVLGTVFMLIGQ 300 PGQVAS+DDI NI+L NV+GV IRIRDVAEV IGRELRTGAATENGREVVLGTVFMLIG+ Sbjct: 241 PGQVASIDDIGNIVLANVKGVPIRIRDVAEVEIGRELRTGAATENGREVVLGTVFMLIGE 300 Query: 301 NSRTVSQAV---------------------------DKKMAEINRSLPEGVQAVT----- 328 NSR VSQAV DK +A + ++L EG V Sbjct: 301 NSRKVSQAVARKMEEINRSLPKGVEAVTVYDRTVLVDKAIATVKKNLVEGALLVVAVLFV 360 Query: 329 -------------------VYDRTVLVDKAISTVKKNL--------IEGAVLVIA----- 356 ++ T +V +S +L I+GAV+++ Sbjct: 361 FLGNIRAAIITAMVIPLAMLFTFTGMVSNKVSANLLSLGALDFGIIIDGAVVIVENCVRR 420 Query: 357 ---VLXHHGRPLTRSERFHEVFAASREARRPLLFGQLIIMIVYLPMFALTGVEGKMFQPM 413 HHGR LTRSERFHEVFAA++EARRPL+FGQLIIMIVYLPMFALTGVEGKMF PM Sbjct: 421 LAHAQAHHGRTLTRSERFHEVFAAAKEARRPLIFGQLIIMIVYLPMFALTGVEGKMFTPM 480 Query: 414 AFTVVAALLGAMILSVTFIPAAIALFIGDKVDEKENALMRAAKRIYAPLLQASLRNKPVV 473 AFTVVAAL+GAMILSVTFIPAAIALFIG+KV EKEN LM AKR+Y P+L+ + NKPVV Sbjct: 481 AFTVVAALIGAMILSVTFIPAAIALFIGEKVAEKENILMLWAKRLYEPMLKTIMENKPVV 540 Query: 474 LSLAAAVIVLCTLLGTKLGSEFVPSLNEGDLAIQALRIPGTSLTQSVEMQQQLERTLKDN 533 L+ A A+I++C ++ ++LGSEFVPSLNEGD+AIQALRIPGTSL+QS+EMQ+Q+E+TLK Sbjct: 541 LTFAGALILVCGVVASRLGSEFVPSLNEGDMAIQALRIPGTSLSQSLEMQKQIEKTLKQK 600 Query: 534 FPEIER--XRTG 543 FPE+ER RTG Sbjct: 601 FPEVERVFARTG 612 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 115 bits (289), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 156/654 (23%), Positives = 261/654 (39%), Gaps = 96/654 (14%) Query: 18 LAVFGLAAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLETEQRISFPIETVMSGLP 77 L + + G+ +Y+ LP+ A+P++ +Q+ T PG SP ++ P+E +P Sbjct: 16 LLMLAILLVGIVAYRLLPLSALPEVDYPTIQVVTLYPGASPDVMTSSVTAPLERQFGQMP 75 Query: 78 NLDQTRSLSRYGLSQVTVIFKDGTDIYFARQLVNERIQEARSKLPSGIDPAMGPISTGLG 137 L Q S S G S +T+ F + A Q V I + LPS D M P+ + Sbjct: 76 GLKQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGSNLLPS--DLPMPPVYS--- 130 Query: 138 EIYLWTVEAKEGAKNADGVPYSPADLREIQDWI---IKPQLRNVPGVTEINSIGGYAKEY 194 V + V + L ++QD I I ++ + GV ++ GG Sbjct: 131 -----KVNPADTPIITLAVTSNTMPLPKVQDLIDTRIAQKISQLSGVGLVSLSGGQRPAV 185 Query: 195 HVAPSPAKLASYGLTLQNVAAALERNNANVGAGYIEKRGEQYLIRAPGQVASLDDIRNII 254 + +P +A+ GL L +V A+ N N G + I A Q+ S D+ RN+I Sbjct: 186 RMQLNPRAVAALGLNLDDVRTAIGNANVNQAKGSFDGPTRASTIDANDQLRSADEYRNLI 245 Query: 255 LGNVQGVAIRIRDVAEVGIGRELRTGAATENGREVVLGTVFMLIGQNSRTVSQAVDKKMA 314 + G IR+ DVA+V G E A N + V+ + G N V ++ K + Sbjct: 246 IAYKNGAPIRVSDVADVVDGAENVRLGAWANTQPAVIVNIQRQPGANVIAVVDSIKKILP 305 Query: 315 EINRSLPEGVQAVTVYDRTVLVDKAISTVKKNLIEGAVLVIAVLXHHGR----------- 363 ++ SLP + + DRT + ++ VK L+ LV+ V+ R Sbjct: 306 QLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSVALVVMVIFVFLRNVPATIIPSVA 365 Query: 364 -PLTRSERFHEVFAAS---------------------------------REARRPL---- 385 PL+ F ++ A E +P+ Sbjct: 366 VPLSLVGTFAIMYLAGFSINNLTLMALTIATGFVVDDAIVMIENISRYIEEGMKPMQAAL 425 Query: 386 ----LFGQLIIMIVY------LPMFALTGVEGKMFQPMAFTVVAALLGAMILSVTFIPAA 435 G II + + +P+ + V G++F A T+ ++L + ++S+T P Sbjct: 426 KGAEQIGFTIISLTFSLIAVLIPLLFMGDVVGRLFHEFAVTLAVSILISAVVSLTLTPMM 485 Query: 436 IALFIGDKVDEKENALMRAAKRI-------YAPLLQASLRNKPVVLSLAAAVIVLCTLLG 488 A + + +E++ +++ Y +LQ L + + L +AAA + L LL Sbjct: 486 CARLLRHQPEEQQGWFYHKSQQFFDRIIARYGVMLQWVLARQKLTLLVAAATLGLTVLLY 545 Query: 489 TKLGSEFVPSLNEGDLAIQALRIPGTSLTQSV--EMQQQLER-TLKD-------NFPEIE 538 + F P + G IQ + S++ S E QQQL + L+D +F ++ Sbjct: 546 VFVPKGFFPLQDTG--VIQGISEATQSISFSAMGEKQQQLAKVVLQDPAVESLTSFIGVD 603 Query: 539 RXRTGKNPDRLLDRLGRP--VREPAVGNTTPANRRAGRLAVGVRAAVCDVQQRQ 590 N R+L L +P VRE V T R G+LA V +Q Q Sbjct: 604 GTNATLNSGRMLINL-KPHDVRE--VSATDVIRRLQGKLAAEVPGITLYMQPVQ 654 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 103 bits (258), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 95/343 (27%), Positives = 162/343 (47%), Gaps = 19/343 (5%) Query: 27 GVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLETEQRISFPIETVMSGLPNLD-QTRSL 85 G+ S + L ++ PDI +V I + G S E ++ IE M GL NL + S Sbjct: 23 GMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDSVTQVIEQQMKGLDNLQYMSASS 82 Query: 86 SRYGLSQVTVIFKDGTDIYFARQLVNERIQEARSKLPSGIDPAMGPISTGLGEIYLWTVE 145 S G + V++ F GT+ A+ V ++Q+ ++LP + ++ +I + Sbjct: 83 SSAGSATVSLTFTAGTNPDVAQMQVQNKLQQVTARLPQAVQNNGVTVTKASTDILMVL-- 140 Query: 146 AKEGAKNADGVPYSPADLREIQDWIIKPQLRNVPGVTEINSIG-GYAKEYHVAPSPAKLA 204 + +D + D+ + + Q+ V GV ++ + G YA + PAKL Sbjct: 141 ----SLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGTNYAMRIWI--DPAKLQ 194 Query: 205 SYGLTLQNVAAALERNNANVGAGYI----EKRGEQY--LIRAPGQVASLDDIRNIIL-GN 257 Y L ++ +AL+ N V AG I + G+Q I A ++ + D+ RNII+ + Sbjct: 195 KYALMPSDITSALQAQNTEVSAGEIGALPAQPGQQINATITARSKLTTPDEFRNIIVKSS 254 Query: 258 VQGVAIRIRDVAEVGIGRELRTGAATENGREVVLGTVFMLIGQNSRTVSQAVDKKMAEIN 317 V G +++ DVA V +G E A+ +NG+ + + G N+ + AV KK+AE+ Sbjct: 255 VDGSVVKLSDVARVELGGESYQVASQQNGKPSSALAIQLATGANALATADAVKKKLAELE 314 Query: 318 RSLPEGVQAVT--VYDRTVLVDKAISTVKKNLIEGAVLVIAVL 358 P ++ T YD T V ++ V K LIE VLV+ ++ Sbjct: 315 PYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVLIM 357 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 103 bits (256), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 95/360 (26%), Positives = 158/360 (43%), Gaps = 15/360 (4%) Query: 7 RFAIEHRWLVLLAVFGLAAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLETEQRIS 66 RF I+ + + +GV + +LPI P + V IN + PG +P + Sbjct: 3 RFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDGVI 62 Query: 67 FPIETVMSGLPNLDQTRSLS-RYGLSQVTVIFKDGTDIYFARQLVNERIQEARSKLPSGI 125 IE +SG+ NL S + G +Q++V FK GTD A+ V +++ ++LP + Sbjct: 63 SLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQTV 122 Query: 126 DPAMGPISTGLGEIYLWTVEAKEGAKNADGVPYSPADLREIQDWIIKPQLRNVPGVTEIN 185 + + L + DG Y L + I +LR + GV + Sbjct: 123 RQNGVTVESAASSFLLMV-----SLTSVDG-RYDEVRLNDYLARNIVEELRRMEGVGRVQ 176 Query: 186 SIGGYAKEYHVAPSPAKLASYGLTLQNVAAALERNNANVGAGYI----EKRGEQYL--IR 239 G + + P KL +YGLT+ +++ A+ + NA + G + RG+Q + Sbjct: 177 LFGA-EQAMRIWLDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLT 235 Query: 240 APGQVASLDDIRNIIL-GNVQGVAIRIRDVAEVGIGRELRTGAATENGREVVLGTVFMLI 298 GQ+ S I+L G + + DVA V +G + A ENG+ + V + Sbjct: 236 VQGQLQSPAQFAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQLSP 295 Query: 299 GQNSRTVSQAVDKKMAEINRSLPEGVQAVTVYDRTVLVDKAISTVKKNLIEGAVLVIAVL 358 G N+ + A+ +M E+ SLP+G+ Y+ V +I V + LIE VLV V+ Sbjct: 296 GANAVRTAAAIQARMEELRPSLPQGMAFSLPYNTAPFVKISIQKVVQTLIEAMVLVFLVM 355 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 99.0 bits (245), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 126/458 (27%), Positives = 198/458 (43%), Gaps = 60/458 (13%) Query: 7 RFAIE---HRWLVLLAVFGLAAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLETEQ 63 RF I+ W+ LA+ + A G+ S LPI P I + I + PG S E Sbjct: 3 RFFIDRPIFAWV--LAIIVMLA-GIVSVLTLPIAQYPAIAPPAIAITANYPGASAKTLED 59 Query: 64 RISFPIETVMSGLPNLDQTRSLSRY-GLSQVTVIFKDGTDIYFARQLVNERIQEARSKLP 122 ++ IE M GL L S S G +T+ F++GT+ A+ V ++ A LP Sbjct: 60 TVTQIIEQKMKGLDRLSYMASTSESSGSVTITLTFENGTNPDTAQVQVQNKLSLATPLLP 119 Query: 123 SGIDP---AMGPISTGLGEIYLWTVEAKEGAKNADGVPYSPADLREIQDWIIKPQLRNVP 179 S + + +T + +T E DG S +DL + ++ + V Sbjct: 120 SEVQQQGITVTKSATNFLNVLAFTSE--------DG-SMSGSDLSDYVAANVQDAISRVE 170 Query: 180 GVTEINSIGG-YAKEYHVAPSPAKLASYGLTLQNVAAALERNNANVGAGYI------EKR 232 GV + G YA + P+ KL SYGLT +V A++ NA V +G + E + Sbjct: 171 GVGDTTLFGSQYAMRIWLDPN--KLNSYGLTPLDVKNAIQAQNAQVSSGQLGGLPAAENQ 228 Query: 233 GEQYLIRAPGQVASLDDIRNIILGNVQGVA-IRIRDVAEVGIGRELRTGAATENGREVVL 291 I A ++ + D +NI+L + G A +++RDVA + +G E T NG+ Sbjct: 229 QINATITAQTRLRTADQFQNIVLRTLTGGAQVKLRDVARIELGSESYTSVGRYNGKPAAG 288 Query: 292 GTVFMLIGQNSRTVSQAVDKKMAEINRSLPEGVQAVTVYDRTVLVDKAISTVKKNLIEGA 351 + + G N+ +A+DK++AE+ + P G++ YD T V +I V + LIE Sbjct: 289 LAIKLATGANALDTVKAIDKRVAELEKFFPTGMKVQKPYDTTPFVRISIEEVVRTLIEAV 348 Query: 352 VLVIAVLXHHGRPLTRSERFHEVFAASREARRPLLFGQLIIMIVYLPMFALTGVEGKMFQ 411 VLV V+ F + F R L + + +V L F + V G Sbjct: 349 VLVFLVMY----------LFLQNF-------RATLIPTIAVPVVLLGTFGVLAVFG---- 387 Query: 412 PMAFTVVAALLGAMILSVTFIPAAIALFIGDKVDEKEN 449 FT+ + AM+L AI L + D + EN Sbjct: 388 ---FTINTLTMFAMVL-------AIGLLVDDAIVVVEN 415 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,113,447 Number of extensions: 87064 Number of successful extensions: 327 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 307 Number of HSP's successfully gapped: 13 Length of query: 718 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 626 Effective length of database: 1,184,250 Effective search space: 741340500 Effective search space used: 741340500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)