BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0010.p (155 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 225 1e-60 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 225 bits (574), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 109/142 (76%), Positives = 126/142 (88%), Gaps = 2/142 (1%) Query: 1 LRFNELISGVRSDVAVKVFGDDMDVLNDTAAKIAATLGKVPGATEVKVEQSTGLPMLTVK 60 LRFNELISGVRSDVAVKVFGDDM VLNDTA+KI+A L K+PGATEVK+EQ+TGLPMLTV+ Sbjct: 672 LRFNELISGVRSDVAVKVFGDDMKVLNDTASKISAVLEKIPGATEVKIEQTTGLPMLTVE 731 Query: 61 IDREKTARYGLNIGDVQDTVAMAIGGKEAGTLFQGDRRFDIIVRLPENERNDLDAIKRLP 120 IDREKTARYGLN+ D+QDTVA AIGGKEAGT+FQGDRRF I+VRLP+ R DL+AI+RLP Sbjct: 732 IDREKTARYGLNVSDIQDTVATAIGGKEAGTMFQGDRRFGIVVRLPDQARTDLEAIRRLP 791 Query: 121 IPRPQADG--KLSYIPLGEVAD 140 I P +G ++SY+PLGEVA Sbjct: 792 IALPPQEGATRVSYVPLGEVAS 813 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.135 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 470,441 Number of extensions: 18558 Number of successful extensions: 61 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 61 Number of HSP's successfully gapped: 1 Length of query: 155 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 77 Effective length of database: 1,250,540 Effective search space: 96291580 Effective search space used: 96291580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 126 (53.1 bits)