BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0003.p (319 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC tra... 129 2e-31 gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease ... 116 2e-27 gi|300312636|ref|YP_003776728.1| sugar ABC transporter permease ... 105 7e-24 gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease ... 95 7e-21 gi|300313833|ref|YP_003777925.1| sugar ABC transporter permease ... 86 5e-18 >gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 129 bits (325), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 88/290 (30%), Positives = 146/290 (50%), Gaps = 5/290 (1%) Query: 27 KFAALADNWLPRLVLSPTIILSLVFVYGFIALTGYLSLTNSRLMPNY-EFAGFDQYTRLF 85 K A WLP L+++P II++L+F + Y S+ Y EF FD + LF Sbjct: 3 KRARFTSKWLPYLLVAPQIIITLLFFFWPAVQALYQSMLLQDAFGGYSEFVWFDNFKTLF 62 Query: 86 ALDRWWTAASNLGIFGGLFILLCLAIGLFMAILLDQKIRAEGALRAVYLYPMALSFIVTG 145 + + IF L LA+ L +A+ D+ R + ++P A+S +V G Sbjct: 63 GDPTYLESFRTTAIFSALVAFFGLALSLLLAVFADRVRRGASIYKTFLIWPYAVSPVVVG 122 Query: 146 AAWKWILNPGLGLEKMMHDWGFLNFHFDWLVNSDMAIYTVVIAGVWQSSGFVMALFLAGL 205 W ++L+P LG+ + W L +D+++N A+ +VIA +W+ + FLAGL Sbjct: 123 VLWMFLLSPSLGILSHVLSW--LGLPWDYMINGTHAMILIVIAAIWKQISYNFLFFLAGL 180 Query: 206 RGIDDSIIKAAMVDGASLPTIYRRIVIPALRP-VFFSVLLVLGHIAIKSFDLVMALTAGG 264 + I S+I+AA +DGA + IV P + P FF ++ + + +F +V A T GG Sbjct: 181 QSIPKSLIEAAAIDGAGPVKRFFTIVFPLISPTTFFLFVVNVVYAFFDTFVIVDATTHGG 240 Query: 265 PGNSSDLPAIFMYQFSFTRGQLGLGAASAVMMLA-TVLAVLVPLMYLETK 313 PG +++ ++ F G LG AA +V+++A +L +V Y+E K Sbjct: 241 PGKDTEILVYKVFSDGFKGGDLGGSAAQSVVLMALVILLTVVQFKYVEKK 290 >gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 311 Score = 116 bits (290), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 80/265 (30%), Positives = 135/265 (50%), Gaps = 6/265 (2%) Query: 41 LSPTIILSLVFVYGFIALTGYLSLTNSRLMPNYEFAGFDQYTRLFALDRWWTAASNLGIF 100 L+P +++LV+V I T YLS + M F G Y LF ++TA N + Sbjct: 42 LAPACVMTLVYVLYPILYTIYLSFFSWDGMTEPRFVGLANYVELFHAPTFYTALKN-NVL 100 Query: 101 GGLFILLCLAIGLFMAILLDQKIRAEGALRAVYLYPMALSFIVTGAAWKWILNPGLGLEK 160 L LL +GL +A+ L+QK+ +++++ P LS +V G + W +P GL Sbjct: 101 WLLMFLLAPPMGLAIALYLNQKVIGMRVVKSLFFAPFVLSGVVVGLMYSWFYDPSFGLLS 160 Query: 161 MMHDWGFLNFHFDWLVNSDMAIYTVVIAGVWQSSGFVMALFLAGLRGIDDSIIKAAMVDG 220 ++ G L ++ A + ++ A +W + + M LFL GL ++ ++AA ++G Sbjct: 161 LLFGQGV-----PVLGDARYATFGIIFAALWPQTAYCMILFLTGLTALNHDQVEAARMEG 215 Query: 221 ASLPTIYRRIVIPALRPVFFSVLLVLGHIAIKSFDLVMALTAGGPGNSSDLPAIFMYQFS 280 A T+ R +++P LR F +V A++SFDL+ +T+GGP SS + A + Y + Sbjct: 216 AKGWTMLRYVILPQLRSTTFMAFVVSIIGALRSFDLISVMTSGGPYESSTVLAYYAYDQA 275 Query: 281 FTRGQLGLGAASAVMMLATVLAVLV 305 + G AA AV + A +L +V Sbjct: 276 IKYYRQGYSAAVAVTLFAIMLVYIV 300 >gi|300312636|ref|YP_003776728.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 299 Score = 105 bits (261), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 83/292 (28%), Positives = 146/292 (50%), Gaps = 12/292 (4%) Query: 34 NWLPRLVLSPTIILSLVFVYGFIALTGYLSLTNSRLMPNYEFAGFDQYTRLFALDRWWTA 93 N L L ++P +IL +VF+ + L +L T++++ F G D +T L + Sbjct: 10 NVLGMLFMAPAVILLVVFLTYPLGLGIWLGFTDTKIGGEGSFIGLDNFTYLAG-----DS 64 Query: 94 ASNLGIFGGLFI-----LLCLAIGLFMAILLDQKIRAEGALRAVYLYPMALSFIVTGAAW 148 + L +F +F +L +GL++AILL++ + + RA+ L P + ++ A+ Sbjct: 65 LAQLSLFNTVFYTVSASILKFMLGLWLAILLNKNVPLKTFFRAIVLLPWIVPTALSALAF 124 Query: 149 KWILNPGLG-LEKMMHDWGFLNFHFDWLVNSDMAIYTVVIAGVWQSSGFVMALFLAGLRG 207 W+ + + +H G ++ + D+L + A ++ V A VW+ FV LAGL+ Sbjct: 125 WWLYDAQFSVISWALHKMGLIDRYIDFLGDPWNARWSTVFANVWRGIPFVAISLLAGLQT 184 Query: 208 IDDSIIKAAMVDGASLPTIYRRIVIPALRPVFFSVLLVLGHIAIKSFDLVMALTAGGPGN 267 I S+ +AA +DGA+ +R + +P L P+ V+ F L+ LT GGP N Sbjct: 185 ISPSLYEAAAIDGATPWQQFRHVTLPLLTPIIAVVMTFSVLFTFTDFQLIYVLTRGGPLN 244 Query: 268 SSDLPAIFMYQFSFTRGQLGLGAASAVMMLATVL-AVLVPLMYLETKGTRNG 318 ++ L A +Q + G LG GAA A M+ +L A++ L+ +G + G Sbjct: 245 ATHLMATLSFQRAIPGGALGEGAALATYMIPFLLAAIMFSYFGLQRRGWQQG 296 >gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 324 Score = 95.1 bits (235), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 87/308 (28%), Positives = 141/308 (45%), Gaps = 11/308 (3%) Query: 3 NSTVSLSSTGTHSSKAAPQSRRSGKFAALADNWLP-RLVLSPTIILSLVFVYGFIALTGY 61 +ST S++ S + P SRR + + P RL+ +P++++ L ++ +A+T Y Sbjct: 5 HSTQVSSTSPMMQSTSQPASRRQQTSPPRKERFNPIRLMQTPSVLILLAWMIVPLAMTIY 64 Query: 62 LSLTNSRLM-PNYE-FAGFDQYTRLFALDRWWTAASNLGIFGGLFILLCLAIGLFMAILL 119 S+ LM P+ F G + Y L +W + N + G + + + G +A+L Sbjct: 65 FSVNRYNLMAPDQTGFVGLENYAFLLQDPAFWPSIGNTALLIGSVLAISVIGGTLLAVLF 124 Query: 120 DQKIRAEGALRAVYLYPMALSFIVTGAAWK-WILNPGLG-LEKMMHDWGFLNFHFDWLVN 177 DQ R G R + + P + V WK I++P G L +M G DWL Sbjct: 125 DQPFRGRGIARLLVIGPFFVMPTVAALIWKNMIMHPVYGILAYVMRHLGLEPI--DWLAE 182 Query: 178 SDMAIYTVVIAGVWQSSGFVMALFLAGLRGIDDSIIKAAMVDGASLPTIYRRIVIPALRP 237 M +V+I WQ F + L L+ +D +AA +DGA I+ IV+P L+ Sbjct: 183 YPM--LSVIIIVAWQWIPFAFLILLTALQSLDTDQKEAAQLDGAGPVRIFFYIVLPHLKR 240 Query: 238 VFFSVLLVLGHIAIKSFDLVMALTAGGPGNSSDLPAIFMYQFSFTRGQLGLGAASAVMML 297 V+++ + F + TAGGPG ++ +Y S Q +G ASA + Sbjct: 241 AITVVIMIETIFLLSVFAEIFTTTAGGPGTATTNLTFLVY--SIGLQQFDIGIASAGGIF 298 Query: 298 ATVLAVLV 305 A VLA +V Sbjct: 299 AVVLANIV 306 >gi|300313833|ref|YP_003777925.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 304 Score = 85.5 bits (210), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 73/240 (30%), Positives = 115/240 (47%), Gaps = 12/240 (5%) Query: 74 EFAGFDQY-TRLFALD---RWWTAASNLGIFGGLFILLCLAIGLFMAILLDQK-IRAEGA 128 F G D Y T LF D +W A N F + + L IGL +A L K +R Sbjct: 46 SFVGLDNYRTILFDPDWSAAFWNAMWNNVKFFCIHMALQNPIGLLLATLFSLKGLRGART 105 Query: 129 LRAVYLYPMALSFIVTGAAWKWILNPGLGL-EKMMHDWGFLNFHFDWLVNSDMAIYTVVI 187 R + P LS ++ G W+ IL+P G+ EK++ G ++ WL A+ T+ + Sbjct: 106 YRTLIFLPTLLSVVIIGFIWQLILSPLWGVGEKLLGTVGLADWFAPWLGQESTALLTLSL 165 Query: 188 AGVWQSSGFVMALFLAGLRGIDDSIIKAAMVDGASLPTIYRRIVIPALRPVFFSVLLVLG 247 VWQ G M L A L + + +++AA +GAS I+ +I +P + P V ++ Sbjct: 166 ISVWQYVGIPMMLIYAALLAVPEEVLEAAHAEGASAMRIFWQIKLPLIYPTLGLVTILTF 225 Query: 248 HIAIKSFDLVMALTA--GGPGNSSDLPAIFMYQFSF-TRGQLG---LGAASAVMMLATVL 301 +FDL+ ++ GP +SDL + Y+ F + Q+G +GAA A +M +L Sbjct: 226 VANFNAFDLIYSVKGALAGPNYASDLLGTYFYRTFFGYQAQIGSPTMGAAVATLMFLVIL 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 871,723 Number of extensions: 32464 Number of successful extensions: 187 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 174 Number of HSP's successfully gapped: 9 Length of query: 319 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 234 Effective length of database: 1,217,395 Effective search space: 284870430 Effective search space used: 284870430 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)