BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0002.p (501 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 318 6e-88 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 266 4e-72 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 197 1e-51 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 107 2e-24 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 94 2e-20 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 318 bits (815), Expect = 6e-88, Method: Compositional matrix adjust. Identities = 187/437 (42%), Positives = 269/437 (61%), Gaps = 5/437 (1%) Query: 5 LKKIDVVGLLDNSKITKFHVQLIFWSFLIMVVDGFDLQAIGYVAPSLIKAWQVSRASFGT 64 L + V +++ + F L+ L +++DGFD+QA+GYVAP++I W VS+AS G Sbjct: 9 LPTVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGP 68 Query: 65 AFSAGLVGMMIGATQGGTLGDRWGRKRTVLAGAVCFGLFTLVTAFATGLNGLIALRFLAG 124 F AGL GM++G+ +GDR+GR+ ++ + F L L T T L+ L+ LRF+ G Sbjct: 69 VFGAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITG 128 Query: 125 IGLGCAVPNAIALNTEYAPKKYRATLVTVMFLGYTIGAAMGG-AAALLIPRFGWQSVFVI 183 GLG +PNA+AL E++P R T + ++ G+T+GAA GG +A LIP +GW +VF + Sbjct: 129 FGLGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFWV 188 Query: 184 GGIAPLVVALLCYFFLPESVRFQVLRQGAIEQARAQMRKWFPHAGITDESVLEVKEEVRS 243 GG PLV+ L +LPES++F VL + Q +RK P+ I D++ + VKE Sbjct: 189 GGAVPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEVVVKETKAE 248 Query: 244 GMSLPHLFKEKRAPFTVLLWICFIANLITLHFIANWLPTVIESTGVPLQRAVIVTSLFQV 303 GM + LF++ RA TVLLW+ NLI L+F++NWLPT+I G AV++ + QV Sbjct: 249 GMPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIGTSLQV 308 Query: 304 GGTFGGIIISRLLDR-GSLKPLVAFFGLAAVTIASIGKFSNSEVMLEVIVFAAGLFVVGT 362 GG G + + R + R G + L + F LA ++IA IGK + +L V V AG +VG Sbjct: 309 GGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIVAGFCIVGG 368 Query: 363 QFGLNALAGSSYPTFIRATGVGWALGIGRIGSILGPVIGGILIALKLPLPQIFLYGAIPM 422 Q +NALAG+ YPT +R+TG+GWALGIGRIGS++GPVIGG LIAL+ +FL A+P Sbjct: 369 QPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASLFLAAAVPA 428 Query: 423 LVGCMSAFLLLRLRSEA 439 L+ SA + RL A Sbjct: 429 LI---SALTIARLHRTA 442 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 266 bits (679), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 174/436 (39%), Positives = 253/436 (58%), Gaps = 6/436 (1%) Query: 5 LKKIDVVGLLDNSKITKFHVQLIFWSFLIMVVDGFDLQAIGYVAPSLIKAWQVSRASFGT 64 +K +DV ++D ++ TKFH ++ L++V DG+DL G V P L+K W +S G Sbjct: 1 MKTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGA 60 Query: 65 AFSAGLVGMMIGATQGGTLGDRWGRKRTVLAGAVCFGLFTLVTAFATGLNGLIALRFLAG 124 S L GMM GA G L DR GRK+ V V F + T ++ FAT RFLAG Sbjct: 61 LGSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAG 120 Query: 125 IGLGCAVPNAIALNTEYAPKKYRATLVTVMFLGYTIGAAM-GGAAALLIPRFGWQSVFVI 183 +G G +PNA+AL EYAPK+ R+TLV +MF GY++G + G ++P FGWQS+F Sbjct: 121 LGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFA 180 Query: 184 GGIAPLVVALLCYFFLPESVRFQVLRQGAIEQARAQMRKWFPHAGITDESVLEVKEEVRS 243 + PLV+ + + LPESV F +LRQG +EQARA + + P A I ++ L + + Sbjct: 181 AAV-PLVLLPVILWKLPESVGF-LLRQGRLEQARAMLTQVDPQARIATDTQLVQADVKGA 238 Query: 244 GMSLPHLFKEKRAPFTVLLWICFIANLITLHFIANWLPTVIESTGVPLQRAVIVTSLFQV 303 G + HLFK+ RA T+++W+ F L+ ++ + +WLP ++ S G L ++ Sbjct: 239 GAPMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNF 298 Query: 304 GGTFGGIIISRLLDRGSL-KPLVAFFGLAAVTIASIGKFSNSEVMLEVIVFAAGLFVVGT 362 GG G I L DR +L K +V+FF + V IA +G F++ +L +++ AG +GT Sbjct: 299 GGVVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLG-FNSPTPVLYLLIIVAGATTIGT 357 Query: 363 QFGLNALAGSSYPTFIRATGVGWALGIGRIGSILGPVIGGILIALKLPLPQIFLYGAIPM 422 Q L A YP IR+TG+GWA G+GR G+I+GP++GG L+A LPL FL AIP Sbjct: 358 QILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAIPG 417 Query: 423 LVGCMS-AFLLLRLRS 437 LV ++ +LR R Sbjct: 418 LVAALAMGLFMLRYRH 433 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 197 bits (502), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 142/463 (30%), Positives = 236/463 (50%), Gaps = 22/463 (4%) Query: 4 GLKKIDVVGLLDNSKITKFHVQLIFWSFLIMVVDGFDLQAIGYVAPSLIKAWQVSRASFG 63 G + +D+ +LD+ + F ++ + L +V+DGFD Q IG+ PSLIK W ++R +F Sbjct: 8 GNQTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFA 67 Query: 64 TAFSAGLVGMMIGATQGGTLGDRWGRKRTVLAGAVCFGLFTLVTAFATGLNGLIALRFLA 123 A +AGLVGM IG+ G DR+GR+ ++ FG+ T + AF+ + + LRF A Sbjct: 68 PAVAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIAILRFCA 127 Query: 124 GIGLGCAVPNAIALNTEYAPKKYRATLVTVMFLGYTIGAAMGGA-AALLIPRFGWQSVFV 182 G+G+G A+P++ + E++P + R VT + +G + G A ++P +GW+ +F+ Sbjct: 128 GLGIGGALPSSTTMTAEFSPARRRTLAVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFL 187 Query: 183 IGGIAPLVVALLCYFFLPESVRFQVLRQGAIEQARAQMRKWFPHAGITDESVLEVKE--- 239 IGG+ P+ ++L +F +PES R+ ++ R +++ D + +E Sbjct: 188 IGGLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQR-MSRPVSADTGFADAREQAI 246 Query: 240 EVRSGMSLPHLFKEKRAPFTVLLWICFIANLITLHFIANWLPTVIESTGVPLQRAVIVTS 299 E R+G LF +A T+ LW+ F NL+ ++ +WLPT++ G+ A + Sbjct: 247 ETRAGFG--ALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGLT 304 Query: 300 LFQVGGTFGGIIISRLLDR-GSLKPLVAFFGLAAVTIASIGK---FSNSEVMLEVIVFAA 355 + GG FG ++ + + R GS P++ LAA A + ++ +L V + Sbjct: 305 AYNFGGVFGALLCALFITRFGSRLPML-LCCLAATASALFLRSLDVASQTTLLVVGIGLH 363 Query: 356 GLFVVGTQFGLNALAGSSYPTFIRATGVGWALGIGRIGSILGPVIGGILIALKLPLPQIF 415 GLFV Q + AL YPT +RATG AL GR+G+IL G ++I Q Sbjct: 364 GLFVNAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALVIT------QGG 417 Query: 416 LYGAIPMLVGCM----SAFLLLRLRSEAIRRGENLTVVAKGEA 454 G + ML G M A L++R + G T+ + A Sbjct: 418 AAGYLAMLAGAMLMVFVALLVVRKHIPRVNAGAAETLKKESHA 460 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 107 bits (268), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 109/394 (27%), Positives = 187/394 (47%), Gaps = 27/394 (6%) Query: 14 LDNSKITKFHVQLIFWSFLIMVVDGFDLQAIGYVAPSLIKAWQVSRASFGTAFSAGLVGM 73 ++N +++H + + D F ++ +V P L+ WQ+S A G ++G +G Sbjct: 33 IENVPFSRWHTKARIIMGSATLFDAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQ 92 Query: 74 MIGATQGGTLGDRWGRKRTVLAGAVCFGL---FTLVTAFATGLNGLIALRFLAGIGLGCA 130 +IGA G + +R+GR V + +V GL ++V A + L RFL GIG+G Sbjct: 93 VIGALFFGWMAERYGR---VPSASVTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGE 149 Query: 131 VPNAIALNTEYAPKKYRATLVTVMFLGYTIG-AAMGGAAALLIPRFGWQSVFVIGGIAPL 189 VP A E + R + L + +G A A L+PRFGW+ +F++GGI + Sbjct: 150 VPVAATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLVPRFGWEYMFLVGGIPGI 209 Query: 190 VVALLCYFFLPESVRFQVLRQGAIEQARAQMRKW---FPHAGI---TDESVLEVKEEV-- 241 +VALL LPES R+ + R G ++ A +R P + TD+ +E + Sbjct: 210 LVALLIT-RLPESPRWLIAR-GRLDDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALA 267 Query: 242 -----RSGMSLPHLFKEKRAPFTVLLWICFIANLITLHFIANWLPTVIEST-GVPLQRAV 295 R S LF T+++W+ + ++ + I NWLP++ ++ +PLQ ++ Sbjct: 268 AGAGKRQRGSWKELFSPVYRGRTIIVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESL 327 Query: 296 IVTSLFQVGGTFGGIIISRLLDR-GSLKPLVAFFGLAAVTIASIGKFSNSEVMLEVIVFA 354 + S+ V + + L+DR G + +A F + + + ++ +E V+ A Sbjct: 328 RMASVSNVLSACAVLGCALLIDRVGRRRWAIASF-ILSGALLALLGVLKAESAWSVMFLA 386 Query: 355 AGLF-VVGTQFGLNALAGSS-YPTFIRATGVGWA 386 + + V+GT L L YPT +RA G G A Sbjct: 387 SSAYAVMGTTTVLLYLYTPEIYPTRMRAIGTGLA 420 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 94.4 bits (233), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 109/398 (27%), Positives = 182/398 (45%), Gaps = 22/398 (5%) Query: 14 LDNSKITKFHVQLIFWSFLIMVVDGFDLQAIGYVAPSLIKAWQVSRASFGTAFSAGLVGM 73 L + + KF +L L+ + D + +IG+ AP++ K + ++ + VGM Sbjct: 12 LQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMFFVGM 71 Query: 74 MIGATQGGTLGDRWGRKRTVLAGAV----CFGLFTLVTAFATGLNGLIALRFLAGIGLGC 129 ++GA G L DR GR R VL GAV CFG+ +AFA L+ALR L GIG+G Sbjct: 72 LLGAFVFGRLADRIGR-RPVLMGAVVIDACFGV---ASAFAPDFTWLLALRLLTGIGVGG 127 Query: 130 AVPNAIALNTEYAPKKYRATLVTVMFLGYTIGAAMGGAAALLIPRFG---WQSVFVIGGI 186 +P + E+ P R + ++ + IG AL+ +G W+ +F + G+ Sbjct: 128 TLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVSWGNDAWRVIFFVTGL 187 Query: 187 APLVVALLCYFFLPESVRFQVLRQGAIEQARAQMRKWFPHAGITDESVLEVKEEVRSGMS 246 P +V ++ F++PES + + R G + AR + + G + + ++ EV Sbjct: 188 -PALVGVVLRFYVPESPMY-LNRSGKSDAARQVLERVAKVNG-SSTPIPPLQPEVVERKP 244 Query: 247 LPHLFKEKRAPFTVLLWICFIANLITLHFIANWLPTVIESTGVPLQRAVIVTSLFQVGGT 306 + LF ++ L++ + I + + +LP + S G R I + Sbjct: 245 MSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFLALVQL 304 Query: 307 FGGIIISRLLDRGSLKP-LVAFFGLAAV--TIASIGKFSNSEVMLEVIVFAAGLFVVGTQ 363 G + + ++R KP LV F L+AV + S+GK + ML V F + Sbjct: 305 PGYALSAYGVERWGRKPTLVGFLVLSAVGCMLYSLGK----DPMLVVGSTLLLSFSLLGT 360 Query: 364 F-GLNALAGSSYPTFIRATGVGWALGIGRIGSILGPVI 400 + L A YPT +RA+G+G A + R G + P I Sbjct: 361 WAALYAFTPEVYPTDLRASGMGTAGAVARFGGLFAPAI 398 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,414,292 Number of extensions: 61378 Number of successful extensions: 472 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 459 Number of HSP's successfully gapped: 21 Length of query: 501 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 412 Effective length of database: 1,198,455 Effective search space: 493763460 Effective search space used: 493763460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)