BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0022.p (89 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312496|ref|YP_003776588.1| transmembrane protein [Herbaspi... 57 4e-10 >gi|300312496|ref|YP_003776588.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 83 Score = 56.6 bits (135), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 33/75 (44%), Positives = 43/75 (57%), Gaps = 1/75 (1%) Query: 2 QRIVPNILWASFLAAIAAEGCFFAWFDPDELLNLVSQR-DRLAAYTVGFFFLWLFCALSG 60 QR + ++W +FL A AAEG FF+ F+PD+L + L AYTVGFF W+ A+ G Sbjct: 5 QRNIAIVIWVAFLMATAAEGIFFSIFNPDDLAMAAGHEWEALGAYTVGFFCFWVSFAICG 64 Query: 61 GLAFLLNLPGQREYL 75 LA L RE L Sbjct: 65 LLAVRLTQTLPRERL 79 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.331 0.143 0.468 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 298,467 Number of extensions: 10502 Number of successful extensions: 28 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 27 Number of HSP's successfully gapped: 1 Length of query: 89 Length of database: 1,619,870 Length adjustment: 59 Effective length of query: 30 Effective length of database: 1,340,505 Effective search space: 40215150 Effective search space used: 40215150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 123 (52.0 bits)