BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0012.p (466 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase ... 441 e-125 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 134 2e-32 gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 70 3e-13 >gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 839 Score = 441 bits (1133), Expect = e-125, Method: Compositional matrix adjust. Identities = 246/449 (54%), Positives = 311/449 (69%), Gaps = 9/449 (2%) Query: 13 CFHCGLPVAPASSWRVTIAGVERAMCCPGCEAVAQSIVDNGFSDYYGTREGFAATAAAQD 72 CFHCG PV +W + I G R +CC GC++VA+ IVD+G D+Y R +A + Sbjct: 16 CFHCGQPVPAGQAWTLEIGGARRELCCVGCQSVARLIVDSGCEDFYLRRTAPSARVDPEQ 75 Query: 73 LVPPELQFYDSEPPDHDAGASAGADTNADTDISETDYTVSNIRCAACIWLIERRLARLPG 132 L+PPEL + P D +A A +E ++ +RC+AC+WLIE+ LARLPG Sbjct: 76 LLPPELALLEQPQPAPDQAHAADAS-------AELVLSIDGLRCSACVWLIEKYLARLPG 128 Query: 133 VYSAQVNAVSERLQVRRDARICKTSDILAALRAIGYAAYPYDPVQHGEQLERSRKTLFRQ 192 + A++N + RL +RRD +C T+ IL LR +GY AYP+DP++ GEQ R+ + LFRQ Sbjct: 129 MQMAEMNVATSRLHLRRDPALCSTATILRGLRGLGYTAYPFDPLRQGEQARRASRRLFRQ 188 Query: 193 LFIAGLSMMQVMMYAVPAYLAGEGGMDADMAGLMRWAGLLLTLPALVYSAQPFFRGAWRD 252 LFIAGLSMMQVMMYAVP Y+ EG +D DM LMRWA L LT+PA+ YSA PFF GAW Sbjct: 189 LFIAGLSMMQVMMYAVPVYMTHEG-IDPDMMSLMRWASLFLTIPAVFYSALPFFTGAWAG 247 Query: 253 IRNCAPGMDVPVALGIAAAFGGSALATWRGEGDVYFDSVTMFIFLLLCSRYLELAARRKA 312 +R APGMD+PVA+GIAAAF SA+ATWRGEG+++FDSV+MFIFLLL SRYLE AARRK+ Sbjct: 248 LRARAPGMDLPVAIGIAAAFLASAIATWRGEGEIWFDSVSMFIFLLLGSRYLEAAARRKS 307 Query: 313 ASALDKLQQSLPASALRMDAYPHA-GTQLVAAAQLCAGDVILVRPGEALAADCIVLDGVS 371 ASAL+++QQ+ PASAL YP T LV AAQL GDVIL RPG+ +AAD +L+G Sbjct: 308 ASALERMQQAFPASALLAPGYPEQRETTLVVAAQLREGDVILARPGDTIAADACLLEGAG 367 Query: 372 SVDLSLLTGESRPVHKGVGDFVPGGAVNIAQALTAKVVRTAGDSTLSVLVGLVNRAGQAK 431 DL+LL+GESRP +G PGGAVN++Q + +VVR G+STL+ L L +AGQ K Sbjct: 368 EFDLALLSGESRPQSFQIGQEAPGGAVNLSQPVFLRVVRATGESTLAALTRLAEQAGQGK 427 Query: 432 PKLALWADRVAAWFVAALLLFAVAVFCAW 460 P LA WAD VA+ FV ALLL A A F AW Sbjct: 428 PALAQWADVVASRFVVALLLLAGATFLAW 456 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 134 bits (337), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 108/362 (29%), Positives = 178/362 (49%), Gaps = 27/362 (7%) Query: 101 DTDISETDYT--VSNIRCAACIWLIERRLARLPGVYSAQVNAVSERLQVRRDARICKTSD 158 D+ + TD + + + CA+C+ +E+ L ++PGV VN +ER + + Sbjct: 3 DSTATRTDISLDIEGMSCASCVMRVEKVLKKIPGVTEVSVNLATERASIVTAGAVAP-EQ 61 Query: 159 ILAALRAIGYAAYPYDPVQHGEQLERSRKTLFRQLFIAGLSMMQVMMYAVPAYLAGEGGM 218 ++AA+ GY A + P E+ + KT + LS + +P LA G+ Sbjct: 62 LIAAIEKAGYHARAHLP----EEAAPTAKTALSSWWPVALSAALSLPLVLPMLLA-PLGV 116 Query: 219 DADMAGLMRWAGLLLTLPALVYSAQPFFRGAWRDIRNCAPGMDVPVALGIAAAFGGS--- 275 + L +W LL P + F+ W +R + MD+ VALG +AA+G S Sbjct: 117 HWSLPPLWQW---LLATPVQFWLGWRFYHAGWGALRARSGNMDLLVALGTSAAYGLSLWQ 173 Query: 276 --ALATWRGEG----DVYFDSVTMFIFLLLCSRYLELAARRKAASALDKLQQSLPASA-L 328 L T G +YF+S + I L+L ++LE A+R+ A A+ L P +A + Sbjct: 174 WLTLPTMPGHAMAMPHLYFESSAVVITLVLLGKWLEGRAKRQTADAIAALNALRPENARV 233 Query: 329 RMDAYPHAGTQL-VAAAQLCAGDVILVRPGEALAADCIVLDGVSSVDLSLLTGESRPVHK 387 R D G + V AQ+ GD++++RPGE + D +V +G S +D ++LTGESRP K Sbjct: 234 RRD-----GQDIEVPLAQVRVGDLVVLRPGERVPVDGVVREGRSHLDEAMLTGESRPQAK 288 Query: 388 GVGDFVPGGAVNIAQALTAKVVRTAGDSTLSVLVGLVNRAGQAKPKLALWADRVAAWFVA 447 GD + G++N L + ++ L+ +V +V +A AK + D+V+A FV Sbjct: 289 QAGDPLTAGSINAEGVLVMETTAIGAETVLARIVRMVEQAQAAKAPIQRLVDQVSAVFVP 348 Query: 448 AL 449 A+ Sbjct: 349 AV 350 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 70.5 bits (171), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 85/354 (24%), Positives = 146/354 (41%), Gaps = 42/354 (11%) Query: 105 SETDYTVSNIRCAACIWLIERRLARLPGVYSAQVNAVSERLQVRRDARICKTSDILAALR 164 E + + + C LI RL+++ GV + + N + L V+ ++ + ++A L+ Sbjct: 63 EEAVFFIQKMDCPTEEKLIRERLSQMSGVGTLEFNLIQRELTVQH--QLPSIAPLVATLK 120 Query: 165 AIGYAAYPYDPVQHGEQLERSRKTLFRQLFIAGLSMM-QVMMYAVPA---YLAGEGGMDA 220 A+ P + L+ AG Q Y +PA L G G+ A Sbjct: 121 ALDML-----PAVKSDSLDAE----------AGRDAPDQAAAYRIPARRWILLGLAGVAA 165 Query: 221 DMAGLMRWAG--------LLLTLPALVYSAQPFFRGAWRDIRNCAPGMDVPVALGIAAAF 272 A ++ W+ + L L A++ + W +RN + M+ ++L A Sbjct: 166 LGAEVLAWSSGDERSLPVIGLALAAILLGGLGTLKKGWIALRNFSLNMNFLMSL---AVI 222 Query: 273 GGSALATWRGEGDVYFDSVTMFIFLLLCSRYLELAARRKAASALDKLQQSLPASALRMDA 332 G + + W + I L + +E + +A +A+ L P A R Sbjct: 223 GAAVIGQW--------PEAAVVIVLFTLAEMIEALSLDRARNAIAGLLAMTPDMATRRGE 274 Query: 333 YPHAGTQLVAAAQLCAGDVILVRPGEALAADCIVLDGVSSVDLSLLTGESRPVHKGVGDF 392 T V A + D + V PGE + D +VL G +S++ + +TGES PV K GD Sbjct: 275 DGVWST--VPARTVTVDDTVRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQAGDP 332 Query: 393 VPGGAVNIAQALTAKVVRTAGDSTLSVLVGLVNRAGQAKPKLALWADRVAAWFV 446 V G +N A +V GDSTL+ +V V +A + + D A +++ Sbjct: 333 VFAGTINEQGAFEMRVTAAQGDSTLARIVKSVQQAQGQRAPTQRFVDNFARYYI 386 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.136 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,363,185 Number of extensions: 54212 Number of successful extensions: 266 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 261 Number of HSP's successfully gapped: 5 Length of query: 466 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 377 Effective length of database: 1,198,455 Effective search space: 451817535 Effective search space used: 451817535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)