BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0028.0007.p
         (516 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311937|ref|YP_003776029.1| poly(3-hydroxyalkanoate) synthe...   800   0.0  
gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthet...    78   1e-15

>gi|300311937|ref|YP_003776029.1| poly(3-hydroxyalkanoate)
           synthetase [Herbaspirillum seropedicae SmR1]
          Length = 832

 Score =  800 bits (2067), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 382/524 (72%), Positives = 422/524 (80%), Gaps = 39/524 (7%)

Query: 1   MATRKTP--AATRKDEAATPQAAPALGIPQLGDVNAYLTDAMQRTFLFLDTLLDRGNNYL 58
           M  RK P  AA     A      P       GDV AYL D +QR+FLFLDTLLDRGN+YL
Sbjct: 1   MINRKKPVSAAPNDGHAHPDDDQPVAASASWGDVPAYLQDCLQRSFLFLDTLLDRGNDYL 60

Query: 59  EHLDQGTPPLLKFEHEVVMDGHDLPNPCNYALLRLQP----------------------- 95
           EHL+QGTPPLLKF HEVV+DGHDLP  CNYALLRLQP                       
Sbjct: 61  EHLEQGTPPLLKFAHEVVLDGHDLPQACNYALLRLQPPAGLPVNPQARPVVVVDPRAGHG 120

Query: 96  --------------GMRAGHTVYFVTFRPEPEDGQTLLTVMDTEALFIEEVIRRHPQCPA 141
                          M+AGHTVYFVTFRPEPEDGQTL+TVMD EA F+EEVIRRHPQC A
Sbjct: 121 PGIGGFKFDSEVGMAMKAGHTVYFVTFRPEPEDGQTLVTVMDAEARFLEEVIRRHPQCSA 180

Query: 142 KPVVIGNCQAGWAMMALNARRPELFGPLMIVGAPVSYWAGSSTLNPMRYSGAQLGGSWLA 201
           KPVVIGNCQAGWAMM L+A RPELFGPLM+VGAP SYWAGSSTLNPMRYSGA LGG+WLA
Sbjct: 181 KPVVIGNCQAGWAMMTLDAVRPELFGPLMMVGAPASYWAGSSTLNPMRYSGANLGGTWLA 240

Query: 202 SMTSDLGADRFDGAYLVENFEKLNPANTLWNKYYNLWAGVDTEAERFLEFERWWGGYFRM 261
           S+ +DLGADRFDGAYLVENFEKLNPANT WNKYYNLWAGVD EA RFLEFERWWGG+FRM
Sbjct: 241 SLAADLGADRFDGAYLVENFEKLNPANTFWNKYYNLWAGVDQEAARFLEFERWWGGFFRM 300

Query: 262 TGSEIESIVENLFVGNKLARGAMMAGDKAIDLRNITSPVVVFASWGDNITPPPQALNWII 321
           TG+EIE+I ENLFVGNKLARG +    K ++LR I++P+VVFASWGDNITPPPQALNWII
Sbjct: 301 TGAEIEAITENLFVGNKLARGELQVRGKPVNLREISAPIVVFASWGDNITPPPQALNWII 360

Query: 322 DTWGDERAIAAAGRTIIYVLHESVGHLGIFVGGSIALKEHDQLVTSLDVIESLPAGLYEM 381
           DTWGDERAIAAAGRTIIYVLHESVGHLGIFVGGS+ALKEHDQLV+SLDVIESLP GLYEM
Sbjct: 361 DTWGDERAIAAAGRTIIYVLHESVGHLGIFVGGSVALKEHDQLVSSLDVIESLPPGLYEM 420

Query: 382 KLEAKAGKDEQRWDQLEPGDYTVHYQHRTMDDIRKLNPEGREEEEMFSTIAQWSEFNANL 441
           KLE K G+  QRWD LEPGDYTVHY+HRTM+D+RKLNPEGREEE +FSTI+QWS+ NA  
Sbjct: 421 KLERKDGRPTQRWDVLEPGDYTVHYEHRTMEDLRKLNPEGREEEAIFSTISQWSQMNAQF 480

Query: 442 YKTYVRPWVKMSATRDIANTMVKLNPLRMQRQLFSDSFFMAPFI 485
           YKT+VRPWV+M+ATR+ AN ++++NPLRMQRQLFSD+   A FI
Sbjct: 481 YKTWVRPWVRMTATRESANALMRMNPLRMQRQLFSDAHPAAAFI 524


>gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase
           [Herbaspirillum seropedicae SmR1]
          Length = 376

 Score = 78.2 bits (191), Expect = 1e-15,   Method: Compositional matrix adjust.
 Identities = 56/213 (26%), Positives = 98/213 (46%), Gaps = 18/213 (8%)

Query: 145 VIGNCQAGWAMMALNARRPELFGPLMIVGAPVSYWAGSSTLNPMRYSGAQLGGSWLASMT 204
           ++G CQ GW    + AR PE    L++ G+P+   AG     P++    +L   +   + 
Sbjct: 159 LVGLCQGGWISAMIAARFPEKVARLVLAGSPIDAGAGE---GPLKQMVQRLPLRFYEDLV 215

Query: 205 SDLGADRFDGAYLVENFEKLNPANTLWNKYYNLWAGVDTEA--ERFLEFERWWGGYFRMT 262
              G     G+++++ ++ ++P    +N++  L   +D  A   +   F  W+     + 
Sbjct: 216 RS-GGGLMRGSFMLQGWKNMHPDEHYFNEHLELLQHIDDPAYLAKRETFAAWYETTVDLP 274

Query: 263 GSEIESIVENLFVGNKLARGAMMAGDKAIDLRNITSPVVVFASWGDNITPPPQALNWIID 322
           G      + ++F  N LARG  +A  + +DLR IT P+ + A   D+IT P Q LN    
Sbjct: 275 GRWYLQAIGHIFKDNLLARGKYVALGQQLDLRQITCPLYLLAGERDDITTPEQVLN---- 330

Query: 323 TWGDERAIAAAGRTIIYVLHESV--GHLGIFVG 353
                 A A  G     +  + V  GH+G+F+G
Sbjct: 331 ------AAALVGTPAAQITQKIVPGGHIGLFMG 357


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.135    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,692,542
Number of extensions: 76270
Number of successful extensions: 190
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 188
Number of HSP's successfully gapped: 2
Length of query: 516
Length of database: 1,619,870
Length adjustment: 89
Effective length of query: 427
Effective length of database: 1,198,455
Effective search space: 511740285
Effective search space used: 511740285
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 133 (55.8 bits)