BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0009.p (198 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310146|ref|YP_003774238.1| general protein secretion prote... 284 4e-78 >gi|300310146|ref|YP_003774238.1| general protein secretion protein BfpE [Herbaspirillum seropedicae SmR1] Length = 357 Score = 284 bits (726), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 140/197 (71%), Positives = 163/197 (82%) Query: 1 MLPDINRWWARTQFIDTVRLRLYRKIAKMLSNGLPLLKILEELHARASEQGKKSKEPLAI 60 MLPD+NRWWA+ QF DT RLRLYRK++KMLSNGLPLLK+LEEL R S GKK KEPLAI Sbjct: 1 MLPDLNRWWAKAQFTDTARLRLYRKMSKMLSNGLPLLKVLEELRDRESHYGKKPKEPLAI 60 Query: 61 ILDDCRRTVQNGRLLSEGLEPWVPKSEQMILMAGEQSGRLETALLSVVTVVQAGKKINSV 120 ILDDCRR+VQNGRLLSE LE W P++EQMILMAGEQSG+LE L++VV VVQA KKI+SV Sbjct: 61 ILDDCRRSVQNGRLLSEALEGWAPRAEQMILMAGEQSGKLEGTLVAVVNVVQARKKIHSV 120 Query: 121 ILGGIAYPITVLCLTAVYIYLFGTQVIPQFTRMIDPTTWHGPARSLYLMSEWVQQWMLVS 180 I+GG+AYP+ +L L Y+YLFG +VIPQFT M+DP WHG ARSLYLMS WVQQWM + Sbjct: 121 IVGGMAYPLAILALVLSYVYLFGVKVIPQFTLMMDPAKWHGAARSLYLMSLWVQQWMGYT 180 Query: 181 IAGVVALFVLLILSMPR 197 + + L VLLI+SMPR Sbjct: 181 LVALALLIVLLIVSMPR 197 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 587,999 Number of extensions: 21383 Number of successful extensions: 92 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 1 Length of query: 198 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 118 Effective length of database: 1,241,070 Effective search space: 146446260 Effective search space used: 146446260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 128 (53.9 bits)