BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0005.p (116 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310143|ref|YP_003774235.1| type IV Tfp pilus assembly PilO... 146 5e-37 >gi|300310143|ref|YP_003774235.1| type IV Tfp pilus assembly PilO protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 146 bits (368), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 70/86 (81%), Positives = 79/86 (91%) Query: 1 MALHITQIEKHKFVCGLFWQSLSRPRELAREAADLGRKIDSDLMVIRMDHSTAQAGFAQS 60 MA + ++KHKFV GLFWQSLS+PREL REAADLGRKIDSDLMVIRMDH+TAQAGFAQS Sbjct: 1 MASIVITLDKHKFVGGLFWQSLSKPRELNREAADLGRKIDSDLMVIRMDHTTAQAGFAQS 60 Query: 61 KEGVRRGMFSLAAVVSKTLALEGAPY 86 +EG+RRGM+SLAAVVSKTLA+EGA Y Sbjct: 61 REGIRRGMYSLAAVVSKTLAIEGAFY 86 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.133 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 316,800 Number of extensions: 9951 Number of successful extensions: 45 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 45 Number of HSP's successfully gapped: 1 Length of query: 116 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 43 Effective length of database: 1,274,215 Effective search space: 54791245 Effective search space used: 54791245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 124 (52.4 bits)