BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0003.p (165 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310142|ref|YP_003774234.1| type IVB pilus formation outer ... 185 2e-48 >gi|300310142|ref|YP_003774234.1| type IVB pilus formation outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 602 Score = 185 bits (469), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 92/118 (77%), Positives = 105/118 (88%), Gaps = 2/118 (1%) Query: 7 QNTGVASKLSVYTGLENAIKVMLSPYGKVLASPATGAITVVDTPDSLDRVAAYIEGENKA 66 QNT VASKLSVYTG+E+AIKVMLSPYGKVLASPATG+ITVVDTPDSLDR+A YI+GENK+ Sbjct: 290 QNTAVASKLSVYTGIESAIKVMLSPYGKVLASPATGSITVVDTPDSLDRIATYIDGENKS 349 Query: 67 LSRQIAINVTVLSVSLRDADEYGINWSAVYNSLNRRYGISNSF--APSDGAVGFTAGI 122 LSRQIAINVTVLSV+L D D+YGINW+AVY SLN +GI+N++ A S G V FTAGI Sbjct: 350 LSRQIAINVTVLSVTLSDDDQYGINWNAVYRSLNSTFGIANAYEGALSTGLVSFTAGI 407 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.130 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 484,316 Number of extensions: 18278 Number of successful extensions: 38 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 38 Number of HSP's successfully gapped: 1 Length of query: 165 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 87 Effective length of database: 1,250,540 Effective search space: 108796980 Effective search space used: 108796980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 127 (53.5 bits)