BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0034.p (368 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313138|ref|YP_003777230.1| glutamate-1-semialdhyde 2,1-ami... 489 e-139 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 98 1e-21 gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferas... 91 1e-19 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 88 1e-18 gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 79 4e-16 >gi|300313138|ref|YP_003777230.1| glutamate-1-semialdhyde 2,1-aminomutase [Herbaspirillum seropedicae SmR1] Length = 427 Score = 489 bits (1259), Expect = e-139, Method: Compositional matrix adjust. Identities = 249/299 (83%), Positives = 266/299 (88%) Query: 68 RGATRRDKIVKFEGCYHGHADSLLVKAGSGLLTFGNPTSSGVPEDFVKHTLVLDYNNTQQ 127 RGAT RDKI+KFEGCYHGHADSLLVKAGSGLLTFGNPTS+GVPEDF KHTLVLDYN+ Q Sbjct: 128 RGATGRDKIIKFEGCYHGHADSLLVKAGSGLLTFGNPTSAGVPEDFAKHTLVLDYNDAAQ 187 Query: 128 LEEAFKAAGNEIACVIVEPVAGNMNLVPATAEFLQTMRRLCTEYGAILIFDEVMSGFRVA 187 LEEAFK AGNEIACVIVEPVAGNMNLV A+ EFL+TMRRLCTEYGAILIFDEVMSGFRVA Sbjct: 188 LEEAFKTAGNEIACVIVEPVAGNMNLVRASDEFLRTMRRLCTEYGAILIFDEVMSGFRVA 247 Query: 188 LGGAQSLYGIAPDLTALGKVIGGGLPVAAFGGRADIMKHLAPLGGVYQAGTLSGNPVAVA 247 GGAQ L GI PDLTALGKVIGGGLPVAAFGGRA++MKHLAPLGGVYQAGTLSGNPV VA Sbjct: 248 RGGAQELNGIVPDLTALGKVIGGGLPVAAFGGRAEVMKHLAPLGGVYQAGTLSGNPVTVA 307 Query: 248 AGLTTLRIIQEAGFYERLSAQTRKLADGLGDAARDAGVTFSADTLGGMFGLYFSAAVPTS 307 AG+ TL+IIQ+ FY LS QTRKLADGL AA+ AGVTF+AD +GGMFGLYF A VPTS Sbjct: 308 AGMATLKIIQQPDFYTNLSTQTRKLADGLAAAAQAAGVTFAADAIGGMFGLYFDAKVPTS 367 Query: 308 YAEVMQGDKERFNVFFHAMLDQGVYLAPSAFEAGFVSAQHDDAVIAETLVAARTAFAEI 366 YAEVMQGDKERFN FFH MLD GVY APSAFEAGFVSAQH DA+I ET+ AA AF+E+ Sbjct: 368 YAEVMQGDKERFNRFFHKMLDAGVYFAPSAFEAGFVSAQHSDAIIEETVAAAARAFSEL 426 Score = 115 bits (289), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 59/98 (60%), Positives = 69/98 (70%), Gaps = 10/98 (10%) Query: 8 NTNESLFARAQKTTPGGVNSPVRAFRSVGGTPRFITKAQGPYFWDAEERRYIDYIGSWGX 67 TN SLFARAQ++TPGGVNSPVRAFRSVGGTPRFI +A+GP+FWDAE +RYIDYIGSWG Sbjct: 3 KTNASLFARAQQSTPGGVNSPVRAFRSVGGTPRFIERAEGPWFWDAEGKRYIDYIGSWG- 61 Query: 68 RGATRRDKIVKFEGCYHGHADSLLVKAGSGLLTFGNPT 105 IV G H + +A + L+FG PT Sbjct: 62 ------PAIV---GHAHPEVIQAVQQAAARGLSFGAPT 90 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 98.2 bits (243), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 74/224 (33%), Positives = 117/224 (52%), Gaps = 18/224 (8%) Query: 68 RGATRRDKIVKFEGCYHGHAD---SLLVKAGSGLLTFGNPTSSGV---PEDFVKHTLVLD 121 R T R ++ F G +HG +L K L FG P V P H + + Sbjct: 126 RAHTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLGFG-PFPGDVFHAPYPSALHGITSE 184 Query: 122 YNNTQQLEEAFKA--AGNEIACVIVEPVAGNMNLVPATAEFLQTMRRLCTEYGAILIFDE 179 + + ++ FK+ +A +I+EPV G A A+F++ +R LC E+G +LI DE Sbjct: 185 -DALEAVKGLFKSDIEAKRVAAIILEPVQGEGGFYAAPADFMRGLRALCDEHGILLIADE 243 Query: 180 VMSGF-RVALGGAQSLYGIAPDLTALGKVIGGGLPVAAFGGRADIMKHLAPLGGVYQAGT 238 V SG+ R A Y + PDL + K + GG+P++A GRA+IM AP GG+ GT Sbjct: 244 VQSGYGRTGKLFAMEHYDVLPDLMTMAKSLAGGMPLSAVNGRAEIMDAPAP-GGL--GGT 300 Query: 239 LSGNPVAVAAGLTTLRIIQEAGFYERLSAQTRKLADGLGDAARD 282 +GNP+A+A+ L L +++E E+L + ++L D L + ++ Sbjct: 301 YAGNPLAIASALAVLDVMEE----EQLVTRGQRLGDKLQEHLKE 340 >gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 91.3 bits (225), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 66/215 (30%), Positives = 99/215 (46%), Gaps = 21/215 (9%) Query: 64 SWGXRG----ATRRDKIVKFEGCYHGHADSLLVKAGS-GLLTFGNPTSSGVPEDFVKHTL 118 WG + + R +I+ F+ +HG + + +G G T P G P+ + Sbjct: 115 KWGKKNPAADGSARFEIITFKHSFHGRTLATMSASGKDGWDTMFAPQVPGFPKAVL---- 170 Query: 119 VLDYNNTQQLEEAFKAAGNEIACVIVEPVAGNMNLVPATAEFLQTMRRLCTEYGAILIFD 178 LE G V++EPV G ++PA+ EF+Q +R L E +LI D Sbjct: 171 -------NDLESVKALIGEHTVAVMLEPVQGEGGVIPASKEFMQGLRSLTKEKNLLLIVD 223 Query: 179 EVMSGF-RVALGGAQSLYGIAPDLTALGKVIGGGLPVAAFGGRADIMKHLAPLGGVYQAG 237 EV SG R A GI PD+ L K IGGG+P+AA R +I A Q G Sbjct: 224 EVQSGMGRTGQLFAYQHSGIEPDIMTLAKGIGGGVPLAALLAREEI----ACFEAGEQGG 279 Query: 238 TLSGNPVAVAAGLTTLRIIQEAGFYERLSAQTRKL 272 T +GNP+ A G+ ++ + + GF E + + + L Sbjct: 280 TYNGNPLMTAVGVAVIKELLKPGFMESVRERGQYL 314 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 75/242 (30%), Positives = 113/242 (46%), Gaps = 25/242 (10%) Query: 71 TRRDKIVKFEGCYHG--HADSLLVKAGSGLLTFGNPTSSGVPEDFVKHTLVLDYNNTQQL 128 T R +I+ F G YHG +A + L + FG P GV + ++ + + Sbjct: 150 TGRHQIIAFHGGYHGIWNASNALTTGTAYRKGFG-PFMGGVIHAPYPYAYRFPFDTSHKS 208 Query: 129 EEAFK------------AAGNEIACVIVEPVAGNMNLVPATAEFLQTMRRLCTEYGAILI 176 E A +++A VIVEPV G VP + EFLQ +R+ C GA+LI Sbjct: 209 AEQIAGEYVDYLLNTPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLI 268 Query: 177 FDEVMSGF-RVALGGAQSLYGIAPDLTALGKVIGGGLPVAAFGGRADIMKHLAPLGGVYQ 235 DEV +G R A G+ PD+ GK IGG +P+A R+D+ + P G Q Sbjct: 269 VDEVQAGAGRTGKMWAVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKI-PDGS--Q 325 Query: 236 AGTLSGNPVAVAAGLTTLRIIQEAGFYERLSAQTRKLADGLGDAARDAGVTFSADTLGGM 295 T + N ++ A LT + I+Q+ RL R A LG A++ +F++ +G + Sbjct: 326 PNTFAANSISAAVALTNISILQDP----RLDLVNR--AHTLGLEAQERIRSFNSPWVGEV 379 Query: 296 FG 297 G Sbjct: 380 RG 381 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 79.3 bits (194), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 71/236 (30%), Positives = 111/236 (47%), Gaps = 30/236 (12%) Query: 68 RGATRRDKIVKFEGCYHG--HADSLLVKAGSGLLTFGNPTSSGVPEDFVKHTLVLDYNNT 125 +G R +++ E YHG + G TF GV D + HT L+ N Sbjct: 133 KGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGV--DHLPHTHNLEKNAF 190 Query: 126 QQLEEAFKAA-------------GNEIACVIVEPVAGNMNLVPATAEFLQTMRRLCTEYG 172 + E + A + IA VIVEPVAG+ ++ +L+ +R +CT++G Sbjct: 191 TKGEPEYGAHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLKRLREICTKHG 250 Query: 173 AILIFDEVMSGF-RVALGGAQSLYGIAPDLTALGK-VIGGGLPVAAFGGRADIMKHLAPL 230 +LIFDEV++GF R+ A + + PD+ K + G +P+ A + +I H A + Sbjct: 251 ILLIFDEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKPEI--HDAFM 308 Query: 231 GG-----VYQAGTLSGNPVAVAAGLTTLRIIQEAGFYERLSAQTRKLADGLGDAAR 281 G ++ T SG+P+A AAGL +L + Q G E A +AD +AA Sbjct: 309 NGPDGIELFHGYTYSGHPLACAAGLASLEVFQTEGVLENAQA----VADYFQEAAH 360 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,176,024 Number of extensions: 51470 Number of successful extensions: 171 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 9 Length of query: 368 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 281 Effective length of database: 1,207,925 Effective search space: 339426925 Effective search space used: 339426925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)