BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0022.p (773 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 392 e-110 gi|300312322|ref|YP_003776414.1| two component hybrid sensor his... 250 3e-67 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 201 3e-52 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 195 1e-50 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 181 2e-46 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 392 bits (1006), Expect = e-110, Method: Compositional matrix adjust. Identities = 253/574 (44%), Positives = 337/574 (58%), Gaps = 56/574 (9%) Query: 17 LLRPLHTIKGSARMAGAMHLGQHMHDLESRIG------EMMRD-GEPSAALIDDLLARYD 69 L RPLHT+KGSARM GA L +H +E+ + E + D + A ++ LL +D Sbjct: 201 LARPLHTLKGSARMVGARRLSHCLHQMETALRGVQTGIEGVHDRSSATLAALETLLGLHD 260 Query: 70 QGLQLFDALQAPVAIAAAAAAPVSDVTPAALPQAEVAKAPAVPAAAPAPASPAQPPATAP 129 L F AL + +A A+AP +PA P Sbjct: 261 LALDHFAALTQAALLLSADGG---------------ARAPVLPA---------------P 290 Query: 130 LVRIRAGILDSVLNQAGEISISRSGLESEVNVLRQHLTDLGSNVARLSTQLREVEIQAEI 189 L+RIR +LD +LNQ GE+SI+RS L++EV L+ LG + RLS +LR Sbjct: 291 LIRIRTTVLDKLLNQVGEVSIARSRLDNEVGSLQSESKALGEQLMRLSGELR-------- 342 Query: 190 QIAASNQKQPQNQHFDPLEFDRFTHLQELTRMLAESVNDVASLQKNLVDAVDRTQNGLVQ 249 Q Q+ D +Q L LA + + Q+ L + V T+ GL Q Sbjct: 343 ------QWLRQSGTQAQDGGDAAAAMQALAHSLAARLEQASLHQRRLQEGVSSTREGLRQ 396 Query: 250 QARLTRDMQRELMHARMVQFKGVEERLQRLVRQMAKETGKELKLDIVGGAVELDRSILEK 309 QA TR++Q+ELM+ARMV+F +E R+Q LVRQ+A ETGK L LD+ +++DR+ILE+ Sbjct: 397 QAAHTRELQQELMYARMVKFNSMEVRMQHLVRQVAAETGKPLALDLSNCRLQIDRAILER 456 Query: 310 MVGPFEHLLRNAAAHGIEGAEQRLAAGKDVVGRLLLEVRQEGNEAMIRLSDDGQGLDLAR 369 ++GP EHLLRNAA HGIE AEQRLA GK VGRL ++ EGNEA+IR+SDDG+GLDL Sbjct: 457 LMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVSDDGRGLDLEA 516 Query: 370 IHEKALHLGLIVPHQRLSDAELTGIIFHPGFSTSSNVTAIAGRGVGMDVVRSEVASLGGR 429 I +KAL LG S A L +IF PG STS V+A+AGRG+GMDVVRSEVA+LGG Sbjct: 517 IRQKALELG-AAGLAEASQARLADLIFQPGLSTSPQVSALAGRGIGMDVVRSEVAALGGW 575 Query: 430 ISIATVAGKGAQFIIHLPLSLAIAQVVLLELDRQSYAIPSLLVEQVLQIKRADQERMFRE 489 + + + G+G QF ++LPLSLA+ V LL +Q +AIPS+LV+ ++Q++ + E Sbjct: 576 MRVHSQPGQGVQFTLYLPLSLAVQHVCLLRHGQQHFAIPSMLVDSIVQLRGPQARQALAE 635 Query: 490 GLLEWQGRSLPLHYLSALLGDERNIPQQQ---TLPVLVVKSGHDFLAILVDRVVGNREVV 546 LE QG L LH LL DE P + + VL++K +A+L D V GNREVV Sbjct: 636 RSLEHQGSRLALHPWHCLL-DEAPEPAGEGSDSAYVLLIKRDDGLMAVLADHVSGNREVV 694 Query: 547 TKSIGPQLAGMTGVVGATVLGSGSIVLILNPIQL 580 K +GPQLA + GVVGATVLG G IVLI+NP+ L Sbjct: 695 VKPVGPQLATLAGVVGATVLGDGQIVLIINPLLL 728 >gi|300312322|ref|YP_003776414.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 775 Score = 250 bits (638), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 206/633 (32%), Positives = 319/633 (50%), Gaps = 39/633 (6%) Query: 100 LPQAEVAKAPAVPAAAPAPASPAQPPATAPLVRIRAGILDSVLNQAGEISISRSGLESEV 159 LP+ E+A+ VPAA A A A A VR+ A LD +L GL E Sbjct: 168 LPEPELAEPAPVPAAPVAGAHLANASG-ARAVRVSADSLDRLL-----------GLTGES 215 Query: 160 NVLRQHLTDLGSNVARLSTQLREVEIQAEIQIAASNQKQPQNQHFDPLEFDRFTHLQELT 219 V + L +++ R+ RE +Q Q++ D F L Sbjct: 216 LVESRQLKPFSASMLRMKRTQREA-----MQALDLLQQKLATTQLDEFAQTAFAEL---- 266 Query: 220 RMLAESVNDVASLQKNLVDAVDRTQNGLVQQARLTRDMQRELMHARMVQFKGVEERLQRL 279 R L + + Q + +DA DR L Q+ + E + RM F L R+ Sbjct: 267 RTLMQQNQQLLGEQLSALDAFDRRSVNLSQR------LYDEALACRMRPFADGTAGLARM 320 Query: 280 VRQMAKETGKELKLDIVGGAVELDRSILEKMVGPFEHLLRNAAAHGIEGAEQRLAAGKDV 339 VR + GK ++LDIVG ++DR ILEK+ P HLLRNA HGIE + QR AAGK Sbjct: 321 VRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAPIGHLLRNAVDHGIESSAQRRAAGKPE 380 Query: 340 VGRLLLEVRQEGNEAMIRLSDDGQGLDLARIHEKALHLGLIVPH--QRLSDAELTGIIFH 397 G + LE R +I ++DDG G+D+ ++ + GL +RLS+AEL + Sbjct: 381 QGVIRLEARHSAGMLLIEVADDGAGIDIEQVRSAVVARGLSNQETAERLSEAELLEFLLL 440 Query: 398 PGFSTSSNVTAIAGRGVGMDVVRSEVASLGGRISIATVAGKGAQFIIHLPLSLAIAQVVL 457 P FS VT I+GRGVG+DVV + + G I I T AG+G++F++ LPL+L++ + +L Sbjct: 441 PSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRITTQAGQGSRFLMQLPLTLSVIRSLL 500 Query: 458 LELDRQSYAIPSLLVEQVLQIKRADQERMFREGLLEWQ--GRSLPLHYLSALLGDERNIP 515 +++ + YA P V + L++ + + EG + GR + L +L + Sbjct: 501 VDIAGEPYAFPLAYVNRTLRLPAEALQTL--EGYQHFSHGGRQVGLVSAHQVLQKGQWRS 558 Query: 516 QQQTLPVLVVKSGHDFLAILVDRVVGNREVVTKSIGPQLAGMTGVVGATVLGSGSIVLIL 575 + ++ V+V+ + VD +G R +V + + P+L + V+ ++ G+ +LIL Sbjct: 559 RDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPLDPRLGKIADVLAGALMEDGTPLLIL 618 Query: 576 NPIQLVQRHGYQRILRREQPQGVDGG-----VSRKIVLVVDDSLTVRRVMHRLLTREGYQ 630 + +++ R Q DG +RK VLVVDDSLTVR + +LLT GYQ Sbjct: 619 DVADMLRTVEKLTSSGRLQTVDQDGAAPTTTAARKKVLVVDDSLTVRELERKLLTNRGYQ 678 Query: 631 VVLATDGVDGLHQMQAVRPDIVLLDIEMPRMDGFDLTRNIRDMAATATLPIIMITSRTAA 690 V +A DG+DG + ++A D+V+ DI+MPRMDG +L IR A +LP+++++ + Sbjct: 679 VTVAVDGMDGWNAVRADSFDLVITDIDMPRMDGIELVSLIRGAANLQSLPVMIVSYKDRE 738 Query: 691 KHRERAMELGVNAYLGK-PYQDDVLLDLVHGLI 722 + R+R +E G + Y K + D+ LL V LI Sbjct: 739 EDRQRGLEAGADHYFTKSSFHDESLLQAVTDLI 771 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 201 bits (510), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 119/333 (35%), Positives = 189/333 (56%), Gaps = 2/333 (0%) Query: 245 NGLVQQARLTRDMQRELMHARMVQFKGVEERLQRLVRQMAKETGKELKLDIVGGAVELDR 304 N + Q +R TRD+Q +M RM+ V R R+VR +A + GK+++ G A ELD+ Sbjct: 348 NSVSQLSRNTRDLQESVMSIRMMPMDYVFSRFPRMVRDLAHKLGKQVEFVTHGAATELDK 407 Query: 305 SILEKMVGPFEHLLRNAAAHGIEGAEQRLAAGKDVVGRLLLEVRQEGNEAMIRLSDDGQG 364 ++E++V P HL+RN+ HGIE + R A+GK G L L +G +I ++DDG G Sbjct: 408 GLIERIVDPLTHLVRNSIDHGIELPDARRASGKSEAGTLSLSAEHQGGNIIIEVTDDGGG 467 Query: 365 LDLARIHEKALHLGLIVPHQRLSDAELTGIIFHPGFSTSSNVTAIAGRGVGMDVVRSEVA 424 L+ +I KA G+ V LSDAE+ +IF PGFST+ VT ++GRGVGMDVV+ + Sbjct: 468 LNREKILAKAKQQGMPVT-DNLSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNIL 526 Query: 425 SLGGRISIATVAGKGAQFIIHLPLSLAIAQVVLLELDRQSYAIPSLLVEQVLQIKRADQE 484 ++GG + I + G G I LPL+LAI + + + + Y +P V + LQ D + Sbjct: 527 AMGGAVDIRSSKGFGTTISISLPLTLAILDGMSIRIGEEVYILPLGYVVESLQPLAQDIK 586 Query: 485 RMFREG-LLEWQGRSLPLHYLSALLGDERNIPQQQTLPVLVVKSGHDFLAILVDRVVGNR 543 + +G +L +G LPL L + E + V++++S A+ VD ++G + Sbjct: 587 EISGQGQVLRVRGEYLPLIPLYQIFNIEPSFTDPSQGLVVILESDGKKAALFVDDLIGQQ 646 Query: 544 EVVTKSIGPQLAGMTGVVGATVLGSGSIVLILN 576 ++V K++ + G+ GAT+LG G + LI++ Sbjct: 647 QIVVKNLESNYRKVAGISGATILGDGGVALIVD 679 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 195 bits (496), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 114/329 (34%), Positives = 196/329 (59%), Gaps = 4/329 (1%) Query: 252 RLTRDMQRELMHARMVQFKGVEERLQRLVRQMAKETGKELKLDIVGGAVELDRSILEKMV 311 R+ +MQ +M RM+ V +R RLVR ++++ GKE++L +VG E D++I+E + Sbjct: 504 RIAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNIIEALA 563 Query: 312 GPFEHLLRNAAAHGIEGAEQRLAAGKDVVGRLLLEVRQEGNEAMIRLSDDGQGLDLARIH 371 P H++RN+ HG+EG EQR A+GK +G L + EG++ +I + DDG+G+D A I Sbjct: 564 DPLIHIVRNSLDHGLEGPEQRRASGKPALGSLTISAAHEGDQVVIVIEDDGKGIDPALIK 623 Query: 372 EKALHLGLIVPHQ--RLSDAELTGIIFHPGFSTSSNVTAIAGRGVGMDVVRSEVASLGGR 429 KA GLI Q R+SD + ++F GFST+ V+ ++GRGVGMDVVR+ V + G Sbjct: 624 RKAYEKGLIDEAQLERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGS 683 Query: 430 ISIATVAGKGAQFIIHLPLSLAIAQVVLLELDRQSYAIPSLLVEQVLQIKRADQERMFRE 489 +++ +V+GKG + I LPLS+A+ +V+++E D Q + +P V + +++ R+ Sbjct: 684 LALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQLFGVPMEHVMETVRVPRSSLHFANSS 743 Query: 490 GLLEWQGRSLPLHYLSALLG--DERNIPQQQTLPVLVVKSGHDFLAILVDRVVGNREVVT 547 + +G L L ++ +LG E+ + VLVV+ G + +++D +V+ Sbjct: 744 AKMVLRGHILGLKGINQILGIAAEQKTNADGEVAVLVVRVGGQSVGLVIDDFRETVDVIQ 803 Query: 548 KSIGPQLAGMTGVVGATVLGSGSIVLILN 576 K + L G++ G+ ++G GS++++LN Sbjct: 804 KPLLGILGGLSLYSGSALMGDGSVLMVLN 832 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 181 bits (460), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 131/398 (32%), Positives = 206/398 (51%), Gaps = 24/398 (6%) Query: 193 ASNQKQPQNQHFDPLEFDRFTHLQELTRMLAESVNDVASLQKNLVDAVDRTQNGLVQQAR 252 A ++++ Q Q F +E + L +L L +A L+ R ++ ++A Sbjct: 322 ALDERRAQEQKFIKIEVSKLDQLIDLVGELV-----IAGASARLI--ARRRKDSQFEEAT 374 Query: 253 -----LTRDMQRELMHARMVQFKGVEERLQRLVRQMAKETGKELKLDIVGGAVELDRSIL 307 L ++ + RMVQ V +R R+VR +A++ GK++ L + G ELD+S++ Sbjct: 375 QAIDTLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDKSMV 434 Query: 308 EKMVGPFEHLLRNAAAHGIEGAEQRLAAGKDVVGRLLLEVRQEGNEAMIRLSDDGQGLDL 367 E++ P H++RNA HGIE A +RLAAGK L L E +I + DDG+G+D Sbjct: 435 ERLADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMDR 494 Query: 368 ARIHEKALHLGLIVPHQRLSDAELTGIIFHPGFSTSSNVTAIAGRGVGMDVVRSEVASLG 427 I KA+ GL P LSDAE+ +F PGFST+ VT ++GRGVGMDVVR + +L Sbjct: 495 EAILAKAVAQGLASPDAELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNIEALH 554 Query: 428 GRISIATVAGKGAQFIIHLPLSLAIAQVVLLELDRQSYAIPSLLVEQVLQI---KRADQE 484 G +SI T G+G + LPL+LAI + + + IP +V + + + + Sbjct: 555 GEVSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQQPGSHI 614 Query: 485 RMFREGLLEWQGRSLPLHYLSALLGDERNIPQQQTLPVLVVKSGHDFLAILVDRVVGNRE 544 R+ L + +PL L L ER + +L ++V+ G +LVD ++G + Sbjct: 615 VNVRDEPLPF----VPLRELFDLPARERG---RNSL--VIVQYGPLRAGLLVDGLLGECQ 665 Query: 545 VVTKSIGPQLAGMTGVVGATVLGSGSIVLILNPIQLVQ 582 V K +G + G+ G+T+LG G + LIL+ LV Sbjct: 666 AVIKPLGRLFGKVKGLSGSTILGDGRVALILDIAHLVH 703 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,135,537 Number of extensions: 91670 Number of successful extensions: 596 Number of sequences better than 1.0e-08: 52 Number of HSP's gapped: 564 Number of HSP's successfully gapped: 52 Length of query: 773 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 680 Effective length of database: 1,179,515 Effective search space: 802070200 Effective search space used: 802070200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)