BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0008.p (707 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked s... 627 e-180 gi|300313120|ref|YP_003777212.1| glycolate oxidase iron-sulfur s... 402 e-113 gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbas... 158 1e-39 gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenas... 103 6e-23 gi|300313195|ref|YP_003777287.1| oxidoreductase [Herbaspirillum ... 59 1e-09 >gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked subunit) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 627 bits (1616), Expect = e-180, Method: Compositional matrix adjust. Identities = 309/353 (87%), Positives = 326/353 (92%), Gaps = 3/353 (0%) Query: 57 NPDKAIPSLHRCAEYGKMHVQRGAIKFGTGLSGGAMPIADGVVVSTAKFNKIVSMDKYSR 116 N + + + C V RGA GTGLSGGA PIADGVVVSTAKFN+I+ MDKYSR Sbjct: 60 NEAQVVAIMKTCHALQVQIVPRGA---GTGLSGGATPIADGVVVSTAKFNQILQMDKYSR 116 Query: 117 TAVVQPGVRNLAISEAAAPHGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVL 176 TAVVQPGVRNLAISEAAAPHGLYYAPDPSSQIAC+IGGNVAENSGGVHCLKYGLTVHNVL Sbjct: 117 TAVVQPGVRNLAISEAAAPHGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLTVHNVL 176 Query: 177 RVRVVTIEGDVIELGNAGLDAPGLDLLAVFIGSEGMLGIVTEVTVKLVPKPQQARVIMAS 236 RVR+VTIEG+V+ELG+ LDAPGLDLLAVFIGSEGMLG+VTEVTVKLVPKPQ ARVIMAS Sbjct: 177 RVRMVTIEGEVVELGSGALDAPGLDLLAVFIGSEGMLGVVTEVTVKLVPKPQAARVIMAS 236 Query: 237 FDDVVKGGNAVANVIAAGIIPAGLEMMDKTSSRMVEPFVKAGYDVDAEAILLCESDGTAE 296 FDDVVKGGNAVANVIAAGIIPAGLEMMDKTSSRMVEPFVKAGYD+DAEAILLCESDGT E Sbjct: 237 FDDVVKGGNAVANVIAAGIIPAGLEMMDKTSSRMVEPFVKAGYDIDAEAILLCESDGTVE 296 Query: 297 EVEEEIGRMSEVLNASGATAIAVSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPR 356 EVEEEIGRM++VLNASGATAIA SQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPR Sbjct: 297 EVEEEIGRMTDVLNASGATAIACSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPR 356 Query: 357 KRLAEVLLGIARMEGKYGLRCANVFHAGDGNLHPLILFDANLADEFHRAELFG 409 K+LA+VLLGIA ME KYGLRCANVFHAGDGNLHPLILFDAN+ADEFHRAELFG Sbjct: 357 KKLAQVLLGIAEMEKKYGLRCANVFHAGDGNLHPLILFDANIADEFHRAELFG 409 Score = 165 bits (418), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 74/83 (89%), Positives = 79/83 (95%) Query: 1 LFGAEILELCVEVGGTITGEHGVGIEKINSMCVQYSPEEREAFFKVKRAFDTAFLLNPDK 60 LFGAEILELCVEVGGTITGEHGVGIEKINSMCVQ+SP ERE FFK+KRAFD AFLLNPDK Sbjct: 407 LFGAEILELCVEVGGTITGEHGVGIEKINSMCVQFSPAERETFFKLKRAFDPAFLLNPDK 466 Query: 61 AIPSLHRCAEYGKMHVQRGAIKF 83 AIP+LHRCAEYGKMHVQRG ++F Sbjct: 467 AIPTLHRCAEYGKMHVQRGQLRF 489 >gi|300313120|ref|YP_003777212.1| glycolate oxidase iron-sulfur subunit oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 402 bits (1034), Expect = e-113, Method: Compositional matrix adjust. Identities = 197/260 (75%), Positives = 224/260 (86%) Query: 411 GQEPRGEVLDTRPYSGIIDYEPTELVITARCGTPLSEIDAALAANNQVLAFEPPRFGGVS 470 GQ P GE+LDTR YSGIIDYEPTELVITARCGTPLSEIDAALA +NQ+LA+EPP FGG + Sbjct: 38 GQTPSGELLDTRAYSGIIDYEPTELVITARCGTPLSEIDAALAQHNQMLAWEPPSFGGAA 97 Query: 471 TIGGMVAAGLSGPGRASSGSVRDFVLGADLMDAQGQVLHFGGQVMKNVAGYDVSRLLTGS 530 T+GGMVAAGLSGP RAS+G+VRDFVLGA LMDAQG+ LHFGGQVMKNVAGYD+SRLL GS Sbjct: 98 TVGGMVAAGLSGPARASAGAVRDFVLGAVLMDAQGEPLHFGGQVMKNVAGYDISRLLAGS 157 Query: 531 LGTLGLILQASIKVLPQAFATSTRVFEVDEANAIRLLNEWGGQPLPLSASAWVNGVLTIR 590 LGTLGLIL+ S+KVLP+ A+STRVFE+++ A+RLLN+ GQPLPL ASAWV VLTIR Sbjct: 158 LGTLGLILEVSLKVLPRPIASSTRVFEINQEGALRLLNQSAGQPLPLVASAWVGNVLTIR 217 Query: 591 LSGAQAAVSAAETRLGGTELTNGEDFWRDLREQNHAFFADAEAPLWRISLPSVTPPLALK 650 LSGAQAAV +A +LGGTEL+N +D+W DLREQ H FFADAEAPLWR+SLPSV P+ L Sbjct: 218 LSGAQAAVDSAIAKLGGTELSNPDDYWTDLREQTHPFFADAEAPLWRLSLPSVAAPIDLP 277 Query: 651 GVQLIEWGGAQRWYRGDDGV 670 G QLIEWGGAQRW R + V Sbjct: 278 GRQLIEWGGAQRWLRPEGNV 297 >gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 158 bits (399), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 104/342 (30%), Positives = 170/342 (49%), Gaps = 10/342 (2%) Query: 68 CAEYGKMHVQRGAIKFGTGLSGGAMPIADGVVVSTAKFNKIVSMDKYSRTAVVQPGVRNL 127 C++Y + GA GT L G + + GV V ++ N+++++ TA VQ GV Sbjct: 71 CSQYDTPIIPYGA---GTSLEGHVLALQGGVTVDLSQMNQVLAVHAEDLTATVQAGVTRK 127 Query: 128 AISEAAAPHGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVLRVRVVTIEGDV 187 +++ GL++ DP + ++GG + + G + ++YG N L + VVT +G++ Sbjct: 128 QLNQEIKDTGLFFPIDPGAD--ASLGGMASTRASGTNAVRYGTMKENTLTLTVVTAQGEI 185 Query: 188 IELGN-AGLDAPGLDLLAVFIGSEGMLGIVTEVTVKLVPKPQQARVIMASFDDVVKGGNA 246 I+ G A + G DL V++GSEG LGI+TEVTV+L P+P+ + SF V N Sbjct: 186 IKTGTRAKKSSAGYDLTRVYVGSEGTLGIITEVTVRLYPQPEAISAAICSFPTVADAVNT 245 Query: 247 VANVIAAGIIPAGLEMMDKTSSRMVEPFVKAGYDVDAEAILLCESDGTAEEVEEEIGRMS 306 V I G+ A +E++D+ R + K V+ +LL E G+ V+E+ + Sbjct: 246 VIQTIQMGVPLARVELLDENGVRAINAHDKLSLPVN--PLLLFEFHGSENGVKEQAELVQ 303 Query: 307 EVLNASGATAIAVSQSEAERLRFWSGRKNAFPAAGRISPDYYCM--DGTIPRKRLAEVLL 364 ++ A + +R R W+ R NA+ A ++ P + D +P RLAE +L Sbjct: 304 DIAKEFHALGFEWATRPEDRTRLWTARHNAYFALLQLRPGARAISTDCCVPISRLAECVL 363 Query: 365 GIARMEGKYGLRCANVFHAGDGNLHPLILFDANLADEFHRAE 406 + GL A + H GDGN H ++ D N + RAE Sbjct: 364 ATKADCEEQGLTHAIIGHVGDGNFHVQMMVDPNDPADIARAE 405 Score = 65.9 bits (159), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 42/166 (25%), Positives = 81/166 (48%), Gaps = 9/166 (5%) Query: 390 PLILFDANLADEFHRAELFGXGQEPRGEVLDTRPYSGIIDYEPTELVITARCGTPLSEID 449 P+I + A + E H L G G +D + ++ +L T + G +++ Sbjct: 77 PIIPYGAGTSLEGHVLALQG------GVTVDLSQMNQVLAVHAEDLTATVQAGVTRKQLN 130 Query: 450 AALAANNQVLAFEPPRFGGVSTIGGMVAAGLSGPGRASSGSVRDFVLGADLMDAQGQVLH 509 + +P G +++GGM + SG G++++ L ++ AQG+++ Sbjct: 131 QEIKDTGLFFPIDP---GADASLGGMASTRASGTNAVRYGTMKENTLTLTVVTAQGEIIK 187 Query: 510 FGGQVMKNVAGYDVSRLLTGSLGTLGLILQASIKVLPQAFATSTRV 555 G + K+ AGYD++R+ GS GTLG+I + ++++ PQ A S + Sbjct: 188 TGTRAKKSSAGYDLTRVYVGSEGTLGIITEVTVRLYPQPEAISAAI 233 >gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 103 bits (256), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 94/334 (28%), Positives = 151/334 (45%), Gaps = 16/334 (4%) Query: 68 CAEYGKMHVQRGAIKFGTGLSGGAMPIADG--VVVSTAKFNKIVSMDKYSRTAVVQPGVR 125 CA++ V +G TGL G++P G VV+S + N+I ++D + T V+ G Sbjct: 65 CAQFAVPLVPQGG---NTGLVLGSVPDQQGNAVVLSLRRLNRIRAVDALNNTITVESGCV 121 Query: 126 NLAISEAAAPHGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVLRVRVVTIEG 185 + E AA G + +++ +CTIGGN++ N+GG L+YG T L + VVT +G Sbjct: 122 LQHLQEQAAAAGRLFPLSLAAEGSCTIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQG 181 Query: 186 DVI-ELGNAGLDAPGLDLLAVFIGSEGMLGIVTEVTVKLVPKPQ-QARVIMASFDDVVKG 243 +++ L D G DL +FIG+EG LG++T +KL P+P+ Q + A Sbjct: 182 EIMSSLKGLRKDNTGYDLRDLFIGAEGTLGVITAAVMKLFPQPRAQVTALAALPHPAQAL 241 Query: 244 GNAVANVIAAGIIPAGLEMMDKTSSRMVE---PFVKAGYDV-DAEAILLCESDGTAEEVE 299 G G E+M ++V P + +D + +LL SD +EE Sbjct: 242 QLLQLAQAHCGPALTGFELMSDFCLQLVRKHFPEQRLPFDRPHPQYVLLELSDSESEEHA 301 Query: 300 EEI--GRMSEVLNASGATAIAVSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRK 357 + G + L ++ S A+ W R++ A + D ++P Sbjct: 302 RALFEGLIGTALEQGLVDDAVIAASLAQSRALWRLRESISSAQAHEGKNIK-HDISVPIS 360 Query: 358 RLAEVLLG--IARMEGKYGLRCANVFHAGDGNLH 389 R+AE + + + G R H GDGNLH Sbjct: 361 RIAEFIAATDVLVQQAAPGCRMVTFGHLGDGNLH 394 Score = 57.0 bits (136), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 42/142 (29%), Positives = 65/142 (45%), Gaps = 1/142 (0%) Query: 412 QEPRGEVLDTRPYSGIIDYEPTELVITARCGTPLSEIDAALAANNQVLAFEPPRFGGVST 471 Q+ VL R + I + IT G L + AA ++ G T Sbjct: 89 QQGNAVVLSLRRLNRIRAVDALNNTITVESGCVLQHLQEQAAAAGRLFPLSLAAEGSC-T 147 Query: 472 IGGMVAAGLSGPGRASSGSVRDFVLGADLMDAQGQVLHFGGQVMKNVAGYDVSRLLTGSL 531 IGG ++ G G G+ R+ LG +++ AQG+++ + K+ GYD+ L G+ Sbjct: 148 IGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQGEIMSSLKGLRKDNTGYDLRDLFIGAE 207 Query: 532 GTLGLILQASIKVLPQAFATST 553 GTLG+I A +K+ PQ A T Sbjct: 208 GTLGVITAAVMKLFPQPRAQVT 229 >gi|300313195|ref|YP_003777287.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 1333 Score = 59.3 bits (142), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 82/322 (25%), Positives = 121/322 (37%), Gaps = 67/322 (20%) Query: 111 MDKYSRTAVVQPGVRNLAISEAAAPHGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGL 170 +DK T GV +S+AA G +A DP+S A IGGNVA N+GG + +G Sbjct: 263 LDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSAEASCIGGNVAMNAGGKKAVLWGT 322 Query: 171 TVHNVLRVRVVTIEGD------------------------------------------VI 188 + N+ R+V +GD ++ Sbjct: 323 ALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVEVARFKLEWSHPGEKGQKTEVFKTEIL 382 Query: 189 ELGNAGLDAPGL--DLLAVFIG------SEGMLGIVTEVTVKLVPKPQQARVIMASF--- 237 E+ GL D+ F+ EG G++T L P+Q R + F Sbjct: 383 EISGKKFRKEGLGKDVTDKFLSGLPGVQKEGCDGLITSARWILHKMPKQTRTVCLEFFGQ 442 Query: 238 -DDVVKGGNAVANVIAA-----GIIPAGLEMMDKTSSRMVEPFVKAGYDVDAEAILLCES 291 D + + + + A G I AGLE +D+ R V K+ V + +L+ + Sbjct: 443 ARDAIPSIVEIKDYLDAETKKGGAILAGLEHLDERYLRAVGYATKSKRGVLPKMVLIGDI 502 Query: 292 DGTAEE----VEEEIGRMSEVLNASGATAIAVSQSEAERLRFWSGRKNAFPAAGRISPDY 347 G E E+ RM+ N G +AVS EA R +FW R A + Sbjct: 503 VGDDENAVAAAASEVIRMAN--NRVGEGFVAVS-PEA-RKKFWLDRSRTAAIAKHTNAFK 558 Query: 348 YCMDGTIPRKRLAEVLLGIARM 369 D IP R+ E GI R+ Sbjct: 559 INEDVVIPLNRMGEYTDGIERI 580 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,073,553 Number of extensions: 86710 Number of successful extensions: 307 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 300 Number of HSP's successfully gapped: 8 Length of query: 707 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 615 Effective length of database: 1,184,250 Effective search space: 728313750 Effective search space used: 728313750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)