BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0004.p (190 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 339 7e-95 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 117 8e-28 gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 108 2e-25 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 104 5e-24 gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA lig... 103 6e-24 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 339 bits (870), Expect = 7e-95, Method: Compositional matrix adjust. Identities = 155/179 (86%), Positives = 171/179 (95%) Query: 12 RTGMEIAILDDSCKKLKPFETGEICVRGPAVFMGYHNNPDANAKAFKGDWFHTGDLGHVD 71 RTGM+IAIL+D ++L PFETGEICVRGPAVFMGYHNNP+ANAKAFK DWFHTGDLGHVD Sbjct: 378 RTGMQIAILNDDGQELAPFETGEICVRGPAVFMGYHNNPEANAKAFKHDWFHTGDLGHVD 437 Query: 72 NDGFLYITGRSSDMYISGGSNVYPREIEEALLTHPSVSEVAVLGVPDPKWGESGIAVIVA 131 DGFLYITGRSSDMYISGGSNVYPREIEEALLTHP+VSEVAVLGVPDPKWGESG+AVIV Sbjct: 438 QDGFLYITGRSSDMYISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVC 497 Query: 132 KSGQQADGEALLSHLESRIAKYKWPRRFVFWETMPKSGYGKIVKKQIKSLLEEKGDYRL 190 K+GQQ DGEALL+HLESRI KYKWPRRF++W++MPKSGYGKIVKKQIK+LLEE+G+YRL Sbjct: 498 KAGQQVDGEALLAHLESRIGKYKWPRRFIYWDSMPKSGYGKIVKKQIKALLEEQGEYRL 556 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 117 bits (292), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 60/165 (36%), Positives = 91/165 (55%), Gaps = 2/165 (1%) Query: 16 EIAILDDSCKKLKPFETGEICVRGPAVFMGYHNNPDANAKAFKGD-WFHTGDLGHVDNDG 74 E AILDD + + + GEI +RGP V GY PD AK D +F TGD+G +D G Sbjct: 391 EFAILDDDGQPVPLGQPGEIAIRGPQVMPGYWQRPDETAKVMTPDGFFKTGDIGVMDERG 450 Query: 75 FLYITGRSSDMYISGGSNVYPREIEEALLTHPSVSEVAVLGVPDPKWGESGIAVIVAKSG 134 + I R DM + G NVYP E+E + HP V E A +GVPD GE+ + + V + Sbjct: 451 YTKIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEA-VKLFVVRKD 509 Query: 135 QQADGEALLSHLESRIAKYKWPRRFVFWETMPKSGYGKIVKKQIK 179 + L+ + + + YK P+ F + +PK+ GKI++++++ Sbjct: 510 KTLSAAQLMDYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELR 554 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 108 bits (271), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 60/153 (39%), Positives = 87/153 (56%), Gaps = 1/153 (0%) Query: 31 ETGEICVRGPAVFMGYHNNPDANAKAFKGDWFHTGDLGHVDNDGFLYITGRSSDMYISGG 90 E GEI RG GY N A +AF G WFHTGDLG ++ DG++ I RS D+ ISGG Sbjct: 388 EIGEIMFRGNICMKGYLKNDRATHEAFAGGWFHTGDLGVMNADGYIKIKDRSKDIIISGG 447 Query: 91 SNVYPREIEEALLTHPSVSEVAVLGVPDPKWGESGIAVIVAKSGQQADGEALLSHLESRI 150 N+ E+E+ L HP+V AV+ PD KWGE+ A + K G + L S + Sbjct: 448 ENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGETPCAFVELKEGAEVGAAELTEFCRSHL 507 Query: 151 AKYKWPRRFVFWETMPKSGYGKIVKKQIKSLLE 183 A +K P+ +++ +PK+ GKI K +++ ++ Sbjct: 508 AGFKVPKA-IYFGPLPKTSTGKIQKFELRKRMK 539 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 104 bits (259), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 60/174 (34%), Positives = 94/174 (54%), Gaps = 7/174 (4%) Query: 14 GMEIAILDDSCKKLKPFETGEICVRGP--AVFMGYHNNPDANAKAFKGDWFHTGDLGHVD 71 G + I+D+ + L G I +R P +F+ Y N +A + F GD+ TGDLG +D Sbjct: 375 GHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFLRYWRNEEATREKFAGDFLLTGDLGSMD 434 Query: 72 NDGFLYITGRSSDMYISGGSNVYPREIEEALLTHPSVSEVAVLGVPDPKWGESGIAVIVA 131 G++ GR+ D+ S G + P IEE L+ HP+V AV+GV D E A +V Sbjct: 435 EQGYIRYLGRNDDVITSAGYRIGPAAIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVL 494 Query: 132 KSGQQADGEALLS----HLESRIAKYKWPRRFVFWETMPKSGYGKIVKKQIKSL 181 K G D EAL + H+ +++A +++PR F +P + GKI++K +K + Sbjct: 495 KDGVTPD-EALKAELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKTLKEM 547 >gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] Length = 545 Score = 103 bits (258), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 53/154 (34%), Positives = 90/154 (58%), Gaps = 6/154 (3%) Query: 32 TGEICVRGPAVFMGYHNNPDANAKAFKGDWFHTGDLGHVDNDGFLYITGRSSDMYISGGS 91 TGE+ VR P + GY + +A+ + ++G W HT D+GH+D +G+L +T R D+ +GG Sbjct: 386 TGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQDIGHLDAEGYLQVTDRIKDVIKTGGE 445 Query: 92 NVYPREIEEALLTHPSVSEVAVLGVPDPKWGESGIAVIVAKSGQQADGE-ALLSHLES-- 148 V +IE+ + HP+V+E AV+G+ D KWGE +A++V K A E + H+ + Sbjct: 446 WVSSLQIEDIIARHPAVAETAVIGIQDGKWGERPVALVVLKKDAAAVSEDDIREHVRAAA 505 Query: 149 ---RIAKYKWPRRFVFWETMPKSGYGKIVKKQIK 179 I++Y P R F + ++ GK+ K+ ++ Sbjct: 506 EAGAISRYGVPDRVRFVAELARTSVGKLNKRVMR 539 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 624,073 Number of extensions: 25260 Number of successful extensions: 100 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 87 Number of HSP's successfully gapped: 14 Length of query: 190 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 110 Effective length of database: 1,241,070 Effective search space: 136517700 Effective search space used: 136517700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)