BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0002.p (184 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 226 7e-61 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 226 bits (577), Expect = 7e-61, Method: Compositional matrix adjust. Identities = 109/140 (77%), Positives = 119/140 (85%), Gaps = 1/140 (0%) Query: 1 MNLGRLLSDVARRFPDEPGLIFGDGTADGKGDKIATWKQINDRVDTLAHSLQKLGVTKGD 60 MNLGRLLSDVARRFPDEPGLI G ADG D++ W++IN RVD LAH+L + GV KGD Sbjct: 30 MNLGRLLSDVARRFPDEPGLILAQGAADGS-DRLIPWREINARVDALAHALTRRGVKKGD 88 Query: 61 KMLVHSRNNLQIFESAWAAFKLGMVWVPTNVRITPPEAAYLGQSSGASVMLYDRGFANYV 120 KMLVHSRNN QIFESAWAAFK+G+VWVPTN RITPPEAAYLGQSSGA VMLYDRGFA YV Sbjct: 89 KMLVHSRNNQQIFESAWAAFKMGVVWVPTNFRITPPEAAYLGQSSGACVMLYDRGFAQYV 148 Query: 121 DAVKEISPALKHVIALQDPR 140 DAVK SPAL+HV+AL D R Sbjct: 149 DAVKAASPALEHVVALADAR 168 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 536,124 Number of extensions: 19977 Number of successful extensions: 64 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 63 Number of HSP's successfully gapped: 1 Length of query: 184 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 104 Effective length of database: 1,241,070 Effective search space: 129071280 Effective search space used: 129071280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)