BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0074.p (66 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313442|ref|YP_003777534.1| transmembrane protein [Herbaspi... 107 3e-25 >gi|300313442|ref|YP_003777534.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 66 Score = 107 bits (266), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 50/66 (75%), Positives = 61/66 (92%) Query: 1 MKIIVAIAFVLIIGSLASALLFMMRDKGKSNRTVYALTMRVGFSVLLFILLLIAYRLGWI 60 MKI+VAIAF+LI+ SLASAL+F+MRDKGKSNRTV ALT+RVGFSV LF+ +L++Y+LGWI Sbjct: 1 MKIVVAIAFILILASLASALIFLMRDKGKSNRTVQALTLRVGFSVALFLFILLSYKLGWI 60 Query: 61 QPTGIH 66 QPTGI Sbjct: 61 QPTGIR 66 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.337 0.148 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 155,441 Number of extensions: 3517 Number of successful extensions: 35 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 35 Number of HSP's successfully gapped: 1 Length of query: 66 Length of database: 1,619,870 Length adjustment: 38 Effective length of query: 28 Effective length of database: 1,439,940 Effective search space: 40318320 Effective search space used: 40318320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.7 bits) S2: 123 (52.0 bits)