BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0061.p (470 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum s... 686 0.0 gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbasp... 134 2e-32 >gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum seropedicae SmR1] Length = 459 Score = 686 bits (1770), Expect = 0.0, Method: Compositional matrix adjust. Identities = 338/440 (76%), Positives = 373/440 (84%), Gaps = 1/440 (0%) Query: 30 SDPATARAAAQASEFTLKNGMKVIVKEDHRAPTAVQMVWYKVGSIDETNGATGIAHALEH 89 S P A+A A +F L NGMKVIVKED RAPTAVQMVWYKVG IDE NG TG++HALEH Sbjct: 19 SSPMLAQAEP-ARQFVLGNGMKVIVKEDRRAPTAVQMVWYKVGGIDEVNGLTGVSHALEH 77 Query: 90 MMFKGTRKHKTGEFSRLVAELGGRENAFTNRDYTAYFQQVEHGRLEAVMALEADRMRNLQ 149 MMFKGTR HK GEFSRLVAELGG+ENAFT D+TAYFQQ+E LE VMALEADRM NLQ Sbjct: 78 MMFKGTRNHKVGEFSRLVAELGGQENAFTANDFTAYFQQIEKSHLEKVMALEADRMANLQ 137 Query: 150 FDRNEFAKEIRVVMEERRWRTDDQPQGLMNEALHAAAFVAHPYHHPVVGWMDDLQHMNIG 209 FD EFAKEIRV+MEERRWRTDDQP GL+NEAL+AAA+ AHPYHHPVVGWMDDLQHM++ Sbjct: 138 FDAAEFAKEIRVIMEERRWRTDDQPMGLLNEALNAAAWTAHPYHHPVVGWMDDLQHMSVQ 197 Query: 210 DIEDWYRRWYAPNNATLVVSGDVSAQKVAALAEKYFGPIPAKTILRGKPQNEPEQNGLRR 269 DI WYR+WYAPNNATLVV+GDV AQ+V ALA KYFG IPA+ + GKPQNEP+Q G+RR Sbjct: 198 DIAAWYRQWYAPNNATLVVAGDVDAQRVLALARKYFGKIPARRLPAGKPQNEPQQLGMRR 257 Query: 270 VTVKAPAENPYVILAFKAPTLRDVEQDQDVYALDVLSAVLDGYDNARLNDKLVRTDKVAN 329 VTVKAPAENPYV++A+K P LR VEQDQDVYALDVLSAVLDGYDNARL+ LVRT A Sbjct: 258 VTVKAPAENPYVVMAWKTPALRQVEQDQDVYALDVLSAVLDGYDNARLSASLVRTGGKAT 317 Query: 330 SVGASYDDIARGPTLFTLEGSPAAGTTTAQLETLLRAEVGRIASDGVSEQELQRVKTQLI 389 +VGASY +ARGP LFTLEGSPA G TTAQLE LLR EV RIA +GVSEQELQRVKTQLI Sbjct: 318 AVGASYSGVARGPVLFTLEGSPAEGVTTAQLEGLLRGEVERIAREGVSEQELQRVKTQLI 377 Query: 390 ASQIYKRDSIFGQAMEIGVMEMSGLSHKNIDRIVERLKSVTPQQVQAVAQKYFSDDKLTV 449 ASQ+YKRDS+FGQAMEIG+ME +GL +NIDRI+ERLKSVT QVQAVAQ+YF DD LTV Sbjct: 378 ASQVYKRDSVFGQAMEIGMMETAGLGQQNIDRIIERLKSVTAAQVQAVAQQYFQDDTLTV 437 Query: 450 ATLEPLPLSGKKAGPPPGPL 469 ATL PLPL+GKK PPP L Sbjct: 438 ATLVPLPLAGKKPAPPPAGL 457 >gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbaspirillum seropedicae SmR1] Length = 929 Score = 134 bits (337), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 111/423 (26%), Positives = 194/423 (45%), Gaps = 27/423 (6%) Query: 42 SEFTLKNGMKVIVKEDHRAPTAVQMVWYKVGSIDETNGATGIAHALEHMMFKGTRKHKT- 100 SE+ NG K+++ D PT + Y VGS E G TG+AH LEH+MFKG+ +H + Sbjct: 61 SEYRFANGFKLLLLPDDSQPTVTVNITYLVGSRHENYGETGMAHLLEHLMFKGSPRHPSI 120 Query: 101 -GEFSRLVAELGGRENAFTNRDYTAYFQ--QVEHGRLEAVMALEADRMRNLQFDRNEFAK 157 +FS+ G N T D T Y++ Q L +A+EADRM N + R + Sbjct: 121 PQDFSK----RGMNFNGTTWLDRTNYYETFQASPDNLRWAIAMEADRMLNSKIARKDLDS 176 Query: 158 EIRVVMEERRWRTDDQPQGLMNEALHAAAFVAHPYHHPVVGWMDDLQHMNIGDIEDWYRR 217 E+ VV E + P +M + + + A+ H Y +G D++++ I +++ +YR Sbjct: 177 EMTVVRNEFE-AGETSPTRVMLKRMQSVAYDWHAYGRNTIGARSDIENVRIENLQAFYRT 235 Query: 218 WYAPNNATLVVSGDVSAQKVAALAEKYFGPIPA-KTILRGKPQNEPEQNGLRRVTVKAPA 276 +Y P+NA L+++G A +V ++ FG +P K L EP Q+G R+ ++ Sbjct: 236 YYQPDNAVLLIAGKFDAAQVLQWVDQSFGRLPKPKRTLPAFWTVEPTQDGERQFVIRRRG 295 Query: 277 ENPYVILAFKAPTLRDVEQDQDVYALDVLSAVLDGYDNARLNDKLVRTDKVANSVGASYD 336 + V LA+K P+ D AL + +L N RL+ LV T + Sbjct: 296 DQQLVALAYKMPS----ALHPDATALGFAADILTDTPNGRLHKALVETGMATEVYAMPLE 351 Query: 337 DIARGPTLFTLEGSPAAGTTTAQLETLLRAEVGRIASDGVSEQELQRVKTQLIASQIYKR 396 +A P L + G + + + V ++ + +E+ R+ + S Sbjct: 352 GMA--PGLQMMVAKVKVGGDLDAVRQAMISAVESFSTQPPTPEEVARLHREAANS----F 405 Query: 397 DSIFGQAMEIGVMEMSGLSHKN-----IDRIVERLKSVTPQQVQAVAQKYFSDDKLTVAT 451 +++ ++ V + ++ + I+R +R++ ++ + A A +YF D TV Sbjct: 406 ETLQNNPQQLAVAMSNAIARGDWRLLFIER--DRMQRLSSADIAAAAGRYFRRDNRTVGL 463 Query: 452 LEP 454 P Sbjct: 464 YLP 466 Score = 60.8 bits (146), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 96/433 (22%), Positives = 168/433 (38%), Gaps = 79/433 (18%) Query: 53 IVKEDHRAPTAVQMVWYKVGSIDETNGATGIAHALEHMMFKGT----RKHKTGEFSRLVA 108 ++ + R T + + G G +A E M+ +GT R+ EF+RL Sbjct: 528 LLPKQSRGQTVSVSLHLRFGDAQSLFGQRNVAQLTEAMLMRGTDKLDRRQLADEFARL-- 585 Query: 109 ELGGRENAFTNRDYTAYFQQVEHGRLEAVMALEADRMRNLQFDRNEFAK---EIRVVMEE 165 ++ G + Y Q L +AL + R+ + D EF + E +E Sbjct: 586 KING----------SLYRFQTTRENLVPAIALVGEVFRHPRMDAAEFEQLKNETVAGLEA 635 Query: 166 RRWRTDDQPQGLMNEALHAAAFVAHPYHHPVVGW---------MDDLQHMNIGDIEDWYR 216 R P EAL H H+P W + ++ + + ++D++R Sbjct: 636 GR----KDPDTRAGEALSV-----HFDHYPAGDWRAAQTLEQRIAAVRAVTLEQVKDFHR 686 Query: 217 RWYAPNNATLVVSGDVSAQKVAALAEKYFG----------------PIPAKTILRGKPQN 260 R+Y + L V GD + + ++ FG IPAK +L P Sbjct: 687 RFYGASQGELAVVGDFDPAQARSAIDQAFGGWTSAAPYARVLQTWADIPAKRLLVDTPDK 746 Query: 261 EPEQNGLRRVTVKAPAENPYVILAFKAPTLRDVEQDQDVYALDVLSAVLDGYD-NARLND 319 E NG+ F +R + D D AL V + +L G +RL D Sbjct: 747 E---NGV----------------YFARQNIRLSDTDPDYPALAVANFLLGGSSLKSRLAD 787 Query: 320 KLVRTDKVANSVGASYDDIARGPTLFTLEGSPAAGTTTAQLETLLRAEVGRIASDGVSEQ 379 ++ + D ++ + + A + AA +++ +R E+ DG SE+ Sbjct: 788 RVRQQDGLSYGIYSGLQVAALTDAGHLTIYAIAAPQNLDKVDAAVREEIALALKDGFSEE 847 Query: 380 ELQRVKTQLIASQIYKR---DSIFGQAMEIGVMEMSGLSHKNIDRIVERLKSVTPQQVQA 436 ELQR K+ ++ + +R D++ + + + L + IDR +L S+T QV A Sbjct: 848 ELQRAKSGILQQRNQQRAGNDNVAATWTLLMNQDRNFLWQQEIDR---KLASLTLAQVNA 904 Query: 437 VAQKYFSDDKLTV 449 +KY ++T Sbjct: 905 ALRKYVDPARMTT 917 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,473,250 Number of extensions: 60673 Number of successful extensions: 203 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 198 Number of HSP's successfully gapped: 3 Length of query: 470 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 381 Effective length of database: 1,198,455 Effective search space: 456611355 Effective search space used: 456611355 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)