BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0030.p (127 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313346|ref|YP_003777438.1| diadenosine tetraphosphate (Ap4... 219 4e-59 >gi|300313346|ref|YP_003777438.1| diadenosine tetraphosphate (Ap4A) hydrolase family protein [Herbaspirillum seropedicae SmR1] Length = 126 Score = 219 bits (559), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 100/126 (79%), Positives = 113/126 (89%) Query: 1 LDNCIFCKIAAGEIPSKKVYEDEDVLAFHDINPAAPVHFLIIPKLHVATLADCGEKHAAL 60 +DNCIFCKIAAG+IPSKK+YEDED++AFHDINPAAPVHFLI+P+ HVATLADCGE+HAA+ Sbjct: 1 MDNCIFCKIAAGQIPSKKIYEDEDLIAFHDINPAAPVHFLIVPRQHVATLADCGEQHAAV 60 Query: 61 LGKMALLAPKLAQEQGCGYQLDATGNKTGGFKTLFNTGPDGGQEVYHLHMHVIGGAHPWR 120 LGKM LAP+LA EQGCGY LDA G +GGFKT+ NTGPDGGQEVYHLHMHVIGG HPWR Sbjct: 61 LGKMLALAPRLAAEQGCGYGLDAEGKPSGGFKTIINTGPDGGQEVYHLHMHVIGGPHPWR 120 Query: 121 TKSVLQ 126 + V Q Sbjct: 121 VRFVQQ 126 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.140 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 456,305 Number of extensions: 19136 Number of successful extensions: 40 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 40 Number of HSP's successfully gapped: 1 Length of query: 127 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 52 Effective length of database: 1,264,745 Effective search space: 65766740 Effective search space used: 65766740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)