BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0025.p (304 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine... 503 e-144 gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine... 247 1e-66 gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine... 224 8e-60 gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirill... 88 9e-19 >gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 393 Score = 503 bits (1295), Expect = e-144, Method: Compositional matrix adjust. Identities = 260/294 (88%), Positives = 278/294 (94%) Query: 9 KFFGDQFEDQQQDDKQSSLGSGVIVSPQGYILTNNHVVEAADEIEVALADGRKASAKVVG 68 KFFGDQF++QQQDDKQSSLGSGVIVSPQGYILTNNHVVEAAD+IEVALADGRKASAKVVG Sbjct: 95 KFFGDQFDEQQQDDKQSSLGSGVIVSPQGYILTNNHVVEAADKIEVALADGRKASAKVVG 154 Query: 69 TDPETDLAVIKINLPNLPAITLAHVEQATVGDIVLAIGNPFGVGQTVTMGIISALGRNHL 128 DPETDLAVIKI+LPNLPAITL H E ++VGD+VLAIGNPFGVGQTVTMGI+SALGRNHL Sbjct: 155 IDPETDLAVIKIDLPNLPAITLGHPENSSVGDVVLAIGNPFGVGQTVTMGIVSALGRNHL 214 Query: 129 GINTFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYSRTGGNLGIGFAIPVSTAKTV 188 GINTFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYSRTGGNLGIGFAIP+STAKTV Sbjct: 215 GINTFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYSRTGGNLGIGFAIPMSTAKTV 274 Query: 189 MEAIINHGQVVRGWIGVEPQDITPELAESFGLTKKTGAIIAGVLKNGPADKAGMKPGDIL 248 MEAII+HGQVVRGWIGVEPQDITPELAESFGL KKTGAIIAGVLK GPAD+AGM+PGDIL Sbjct: 275 MEAIISHGQVVRGWIGVEPQDITPELAESFGLGKKTGAIIAGVLKGGPADRAGMRPGDIL 334 Query: 249 ISVQDKPVTDTTEMLNLIAQLVPGQKAKMTVLRKAQETTLEIVVGKRPPPKREE 302 +S+ DKPV DTTEMLN+IAQL PGQ+ MTVLRK+QET L I VGKRPPP + E Sbjct: 335 VSIDDKPVADTTEMLNVIAQLTPGQQIAMTVLRKSQETKLNITVGKRPPPPKNE 388 >gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine endoprotease [Herbaspirillum seropedicae SmR1] Length = 494 Score = 247 bits (630), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 130/295 (44%), Positives = 184/295 (62%), Gaps = 1/295 (0%) Query: 9 KFFGDQFEDQQQDDKQSSLGSGVIVSPQGYILTNNHVVEAADEIEVALADGRKASAKVVG 68 K FG Q + QQ LGSG I+SP G ILTN HVV+ A E+ V L D R+ AKV+G Sbjct: 107 KRFGPQLQMPQQPRIMHGLGSGFIISPDGLILTNAHVVDGAQEVVVKLTDRREFKAKVLG 166 Query: 69 TDPETDLAVIKINLPNLPAITLAHVEQATVGDIVLAIGNPFGVGQTVTMGIISALGRNHL 128 D ++D+AVI+I+ NLP + + + VG VLAIG+P+G T T GIISA R+ L Sbjct: 167 IDKQSDIAVIRIDAKNLPTVQIGDPSRVKVGQPVLAIGSPYGFDNTATAGIISAKSRS-L 225 Query: 129 GINTFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYSRTGGNLGIGFAIPVSTAKTV 188 + + FIQTD A+NPGNSGG L D NG ++GIN+ IYS+TGG G+ F+IP+ A V Sbjct: 226 PDDNYVPFIQTDVAVNPGNSGGPLFDLNGQVIGINSQIYSQTGGFQGLSFSIPIDVAMKV 285 Query: 189 MEAIINHGQVVRGWIGVEPQDITPELAESFGLTKKTGAIIAGVLKNGPADKAGMKPGDIL 248 + ++ HG+V RG +GV QD+ L++SFGL K GA+++ V K PADKAG++ GD++ Sbjct: 286 EQQLVTHGKVTRGRLGVSVQDLNQALSDSFGLKKAEGALVSSVEKGSPADKAGLQAGDVI 345 Query: 249 ISVQDKPVTDTTEMLNLIAQLVPGQKAKMTVLRKAQETTLEIVVGKRPPPKREER 303 IS + + ++ L+A PG M V+R + T+ + VG+ K + + Sbjct: 346 ISFNGHAIDHSVDLPTLVADTPPGSTKPMEVMRAGKVRTINVTVGEMKQAKNDAK 400 >gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 492 Score = 224 bits (570), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 123/314 (39%), Positives = 190/314 (60%), Gaps = 21/314 (6%) Query: 9 KFFGDQFEDQQQ----------------DDKQSSLGSGVIVSPQGYILTNNHVVEAADEI 52 +FFG QQQ ++ +GSG I+S G+I+TN HVVE A E+ Sbjct: 82 RFFGIPIPRQQQPGTPRGNGKQGGQGQQEEVPRGVGSGFIISADGFIMTNAHVVEGASEV 141 Query: 53 EVALADGRKASAKVVGTDPETDLAVIKINLPNLPAITLAHVEQATVGDIVLAIGNPFGVG 112 V L D R+ AK+VG+D TD+AV+KI+ NLP + + ++ VG+ VLAIG+PFG+ Sbjct: 142 YVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLPRLNMGDSDKIRVGEWVLAIGSPFGLE 201 Query: 113 QTVTMGIISALGRNHLGINTFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYSRTGG 172 TVT GI+SA R+ + IQTD A+NPGNSGG L++ G ++GIN+ IYSR+GG Sbjct: 202 NTVTAGIVSAKARD---TGDYLPLIQTDVAVNPGNSGGPLINLKGEVIGINSQIYSRSGG 258 Query: 173 NLGIGFAIPVSTAKTVMEAIINHGQVVRGWIGVEPQDITPELAESFGLTKKTGAIIAGVL 232 +GI FA+P+ A V + + G+V RG IGV+ ++T ++AES GL + GA++ V Sbjct: 259 FMGISFAVPIDEALRVADQLKASGRVTRGRIGVQIGEVTKDVAESLGLARAQGALVQRVE 318 Query: 233 KNGPADKAGMKPGDILISVQDKPVTDTTEMLNLIAQLVPGQKAKMTVLRK--AQETTLEI 290 GPA+KAG++ GDI++ + +++ ++ PG KA +++ RK A++ ++ + Sbjct: 319 AGGPAEKAGLEAGDIILKYNGAAIERPSDLPRMVGSTKPGAKATVSIWRKGSARDVSVTV 378 Query: 291 VVGKRPPPKREERE 304 V + PK+ E + Sbjct: 379 VELEADKPKQAEEK 392 >gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirillum seropedicae SmR1] Length = 438 Score = 88.2 bits (217), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 56/163 (34%), Positives = 94/163 (57%), Gaps = 15/163 (9%) Query: 28 GSGVIVSPQGYILTNNHVVEAADEIEVALADGRKASAKVVGTDPETDLAVIKINLPNLPA 87 G+G + G++L+N HVV A + V L GR+ A+V+ D D+A++K P + Sbjct: 271 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 330 Query: 88 ITLAHVEQATVGDIVLAIGNPFG--VGQTVTMGIISALGRNHLGINTF--ENFIQTDAAI 143 + L +++ +G+ V A+G+P G T+T GI+S G+ + F+Q+D AI Sbjct: 331 LPL-RLDEPGLGEDVYALGSPLGDTFNTTLTRGILS-------GVRLLNGQEFLQSDVAI 382 Query: 144 NPGNSGGALVDTNGNLLGINTA-IYSRTGGNLGIGFAIPVSTA 185 PG+SGG L+D +G ++GI A + +R G G+ F IP+++A Sbjct: 383 LPGSSGGPLLDRSGQVVGITVAGLGAR--GMAGMNFFIPLASA 423 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.135 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 922,220 Number of extensions: 40373 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 4 Length of query: 304 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 219 Effective length of database: 1,217,395 Effective search space: 266609505 Effective search space used: 266609505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)