BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0016.p (366 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-me... 77 3e-15 gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding prote... 70 2e-13 gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [He... 56 4e-09 >gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 76.6 bits (187), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 84/310 (27%), Positives = 128/310 (41%), Gaps = 31/310 (10%) Query: 7 SMEIGILGGGIAGLSVALALHKQGYSPRIYERRAVPATMGAGVTLWPNASFVLGELGLLQ 66 + I I+GGG +G+S A+ L ++G + E A GAG++L P L +LG+L+ Sbjct: 5 AQRILIIGGGFSGMSAAIDLRRRGAQVDLVELDAQWRNYGAGISLGPATLRALKQLGVLE 64 Query: 67 DIEAIGGRPLTMRRQDAAGNAL------GGLDIGLLDRTMGYPTY----TVLRRHLQEVL 116 R+ AAG+ + G L + P +LR L +L Sbjct: 65 ----------AFLREGAAGDGVRLCLPHGPQVAELPTPRLASPDVPGGGAILRPVLARIL 114 Query: 117 LDHAARAGIPVEFGRRAVAIELDAHGRAVAHFENGTSICPDLLIGADGRMESVARKFVAG 176 D AG V G A+ H + F +G + DL+IGADG + Sbjct: 115 ADATRAAGADVRLGCTFSAVREVGH-KVEVDFTDGQTRRYDLVIGADGLYSKLRMHLFPH 173 Query: 177 DNRPIYQGFVNWIGVAQGPHALVDDISIQDFWGAGERFGCVAIRPDLVYWAAAQARPLNE 236 +P Y G W V P +V + + G + G + D +Y + RP+NE Sbjct: 174 APKPRYSGQAVWRAVLPRPQEIV---TCTMWMGPRIKPGVNPVSKDEMYLFVTEPRPVNE 230 Query: 237 -ATPTAGMHKKEVENLFAQWPEPVSNIIRATPENAIRLIAVHDLEPL---HTWSRANVLL 292 PT + + L ++ PV IR ++ R+I LE L W + V+L Sbjct: 231 HVDPTTFV--SHLRGLLEEFSAPVLKTIREQLDDNARII-FRPLEGLLLPRPWYQGRVVL 287 Query: 293 VGDAAHAPLP 302 +GDA HA P Sbjct: 288 IGDAVHATTP 297 >gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 384 Score = 70.5 bits (171), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 77/298 (25%), Positives = 132/298 (44%), Gaps = 44/298 (14%) Query: 28 KQGYSPRIYERRAVPATMGAGVTLWPNASFVLGELGLLQDIEAIGGRP-LTMRRQDAAGN 86 ++G++ R+YE+ + +GAG+ + PN VL +G+ ++ G RP R G+ Sbjct: 25 QEGFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIGIEDEMNRQGSRPDFWYSRHWQTGD 84 Query: 87 ALGGLDIG-LLDRTMGYPTYTVLRRHLQEVLLDHAARAGIPVE---FGRRAVAIELDAHG 142 L + +G + G TV R E+L+ +P E FG+ +E D Sbjct: 85 VLAQIPLGDYAVKHYGASYLTVHRGDFHELLI-----KALPSERLSFGKCLTKVE-DRGD 138 Query: 143 RAVAHFENGTSICPDLLIGADGRMESVARKFVAGDNRPIYQGFVNWIGVAQGPH----AL 198 + F +GT+ D++IGADG + S R+ + G P Y G++ V P L Sbjct: 139 VVILSFADGTTEEADIVIGADG-VNSRIREELLGVEPPKYAGYLAHRAVFPTPELKAGML 197 Query: 199 VDDISIQDFWGAGERFGCVAIRPDLVYWAAAQARPLNEAT--------------PTAGMH 244 D ++ W + +R +VY+ ++A L T P++ Sbjct: 198 PFDACVK--WWSDDRHM-------MVYFVTSKANELYYVTGVPVEKWDLNDRWLPSS--- 245 Query: 245 KKEVENLFAQWPEPVSNIIRATPENAIRLIAVHDLEPLHTWSRANVLLVGDAAHAPLP 302 K+E+ F W V ++ AT E + ++ + +PL WSR ++L+GDA H P Sbjct: 246 KEEMRETFHGWHPTVQALVDATVE--VTKWSLLERDPLPLWSRGRLVLLGDACHPMKP 301 >gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 56.2 bits (134), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 88/315 (27%), Positives = 135/315 (42%), Gaps = 36/315 (11%) Query: 8 MEIGILGGGIAGLSVALALHKQGYSPRIYERRAVPATMGAGVTLWPNASFVLGELGLLQD 67 + + I G G AGL++AL L + G+ + ER A +G+G+ L P VL +LGL Sbjct: 4 LHVAIAGAGPAGLAMALYLKRAGHRVSLLERFLQAAPVGSGLILQPTGLTVLQDLGLFSR 63 Query: 68 IEAIGGRPLTMRRQDA-AGNALGGLDIGLLDRTMGYPTYTVLRRHLQEVLLDHAARAGIP 126 I A+G R + DA +G + LD+ G V R L VLL+ A + GI Sbjct: 64 IAALGQRIDRLYGADALSGKTV--LDVRYDAIAGGRYGIAVHRAALFGVLLEAAQQEGIE 121 Query: 127 VEFGRR--------AVAIELDAHGRAVAHFENGTSICPDLLIGADGRMESVARKFV-AGD 177 VE G+ A A A GR + F DL+I A G + R + + + Sbjct: 122 VETGQDIGNLEHTGAQACLTAADGRRIGGF--------DLVIDASGARSPLRRHALHSCE 173 Query: 178 NRPIYQGFVNWIGV-AQGPHALVDDISIQDFWGAGERFGCVAI-RPD-------LVYWAA 228 RP+ G W+ + AQ + + +Q + A G + I RP +W+ Sbjct: 174 PRPLPYG-AFWVSLRAQPGLSFGHNSLVQRYRRASTMIGVLPIGRPTPQDEPMVAFFWST 232 Query: 229 AQARPLNEATPTAGMHKKEVENLFAQWPEPVSNIIRATPENAIRLIAV-HDLEPLHTWSR 287 R E+ AG+ + + + A WPE + + + L H PL R Sbjct: 233 RPDR--VESLKQAGLSAWK-DGVHALWPECAPYLAQIDGFEQMTLARYGHHTLPLPYGER 289 Query: 288 ANVLLVGDAAHAPLP 302 + ++GDAAH+ P Sbjct: 290 --LAIIGDAAHSTSP 302 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,260,806 Number of extensions: 58593 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 3 Length of query: 366 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 280 Effective length of database: 1,212,660 Effective search space: 339544800 Effective search space used: 339544800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)