BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0009.p (322 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC t... 315 4e-87 gi|300312954|ref|YP_003777046.1| amino acid ABC transporter perm... 133 2e-32 gi|300313932|ref|YP_003778024.1| branched-chain amino acid ABC t... 113 2e-26 gi|300313286|ref|YP_003777378.1| branched-chain amino acid ABC t... 97 2e-21 gi|300311946|ref|YP_003776038.1| branched-chain amino acid ABC t... 95 1e-20 >gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 628 Score = 315 bits (807), Expect = 4e-87, Method: Compositional matrix adjust. Identities = 175/215 (81%), Positives = 200/215 (93%) Query: 1 MTFSSLAVQLINGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGVFIAYTLVSLIG 60 MT +SLAVQL+NGLADASAMF+VAAGLSLIFGVTRIVNFAHGSFYMLG+++AY+LV +G Sbjct: 1 MTLASLAVQLLNGLADASAMFMVAAGLSLIFGVTRIVNFAHGSFYMLGLYLAYSLVDRLG 60 Query: 61 ASGVGFWSAVLLSACAVALLGALVEITVLRRIYRAPELFQLLATFALVLVLKDAALYLWG 120 A+ G+W+A+LL+A VA LGALVE+T+LRRIYRAPELFQLLATFALVLV+KDAAL+ WG Sbjct: 61 ATPWGYWAALLLAALPVAALGALVELTILRRIYRAPELFQLLATFALVLVIKDAALWYWG 120 Query: 121 PEDLFGPRAPGLAGSVELLGRRLPQYDLVLIVIGPAVLGLLWLLLNRTRWGTLIRAATQD 180 PEDLFGPRAPGLAG+V++LGRRLPQYDL+L+ IGP V LLWLLLNRTRWG LIRAATQD Sbjct: 121 PEDLFGPRAPGLAGAVQVLGRRLPQYDLLLMAIGPLVFALLWLLLNRTRWGALIRAATQD 180 Query: 181 REMAGALGIHQSWLFTGVFALGCFLAGLGGALQGP 215 REM GALG++Q+WLFTGVFALGCFLAGLGGALQGP Sbjct: 181 REMLGALGVNQAWLFTGVFALGCFLAGLGGALQGP 215 >gi|300312954|ref|YP_003777046.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 304 Score = 133 bits (335), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 81/213 (38%), Positives = 120/213 (56%), Gaps = 4/213 (1%) Query: 9 QLINGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGVFIAYTLVSLIGASGVGFWS 68 Q++N L +FL+A+GL+LIFGV VN AHGSF+M I + +++ + FW Sbjct: 8 QVLNSLQFGVMLFLIASGLTLIFGVMNFVNLAHGSFFM----IGAYVAAVVAGASGSFWL 63 Query: 69 AVLLSACAVALLGALVEITVLRRIYRAPELFQLLATFALVLVLKDAALYLWGPEDLFGPR 128 +L++ A A LG L+E V RR+Y+ + Q+LATFA +L+ D +WGP+ L Sbjct: 64 GLLVALPATAALGFLLEWVVFRRLYQKDHMVQVLATFAFILIFNDGVRMIWGPQPLPLNM 123 Query: 129 APGLAGSVELLGRRLPQYDLVLIVIGPAVLGLLWLLLNRTRWGTLIRAATQDREMAGALG 188 AG V L G Y L+++ +G V L+ L+NRTR G +RA + EM A+G Sbjct: 124 PAAFAGPVNLFGLPYSSYRLLVMAVGVVVAIALYFLINRTRIGMWVRAGANNLEMVTAMG 183 Query: 189 IHQSWLFTGVFALGCFLAGLGGALQGPXSGMRS 221 I+ LF VF+ G LA L G + GP +++ Sbjct: 184 INIRLLFRLVFSFGLLLAALAGIMLGPLVAVQA 216 >gi|300313932|ref|YP_003778024.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 113 bits (283), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 71/194 (36%), Positives = 114/194 (58%), Gaps = 8/194 (4%) Query: 8 VQLINGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGVFIAYTLVSLIGASGVGFW 67 +Q+ NG+ FL+A GLS++FG+ R VNF+HG+F++LG ++ + + L G+GFW Sbjct: 6 LQIANGVGIGMLYFLLAVGLSIVFGLLRFVNFSHGAFFLLGAYLCFQVTDL----GLGFW 61 Query: 68 SAVLLSACAVALLGALVEITVLRRIYRAPELFQLLATFALVLVLKDAALYLWGPEDLFGP 127 ++L++ V L +VE VLR +Y P F +L T + LV+++A + WGP L Sbjct: 62 WSLLIAPLVVGLFAWIVERFVLRHVYALPHHFHILFTVGMALVIQEAVISTWGP--LGNN 119 Query: 128 RAPG--LAGSVELLGRRLPQYDLVLIVIGPAVLGLLWLLLNRTRWGTLIRAATQDREMAG 185 AP L G V G P+Y L +I + + LLW +L TR G+++RA ++ EM Sbjct: 120 VAPPDLLQGVVMWGGFVYPKYRLFVIGLTAVMALLLWWVLEGTRLGSIVRAGSESTEMVS 179 Query: 186 ALGIHQSWLFTGVF 199 LG++ + +F+ VF Sbjct: 180 LLGLNINRIFSLVF 193 >gi|300313286|ref|YP_003777378.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 97.1 bits (240), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 68/191 (35%), Positives = 106/191 (55%), Gaps = 3/191 (1%) Query: 10 LINGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGVFIAYTLVSLIGASGVGFWSA 69 L +G+A S +FL++ GLS+ G+ +N AHG+F MLG Y V+L+ GV F + Sbjct: 9 LFDGIAYGSLLFLISVGLSVTMGMMNFINLAHGAFAMLG---GYVCVTLLNGMGVPFLAT 65 Query: 70 VLLSACAVALLGALVEITVLRRIYRAPELFQLLATFALVLVLKDAALYLWGPEDLFGPRA 129 + L+ A A++G ++E ++ RR+Y+A L Q+L + L + A + WGP Sbjct: 66 LPLAFIAAAIVGLVLERSLYRRLYKASHLDQVLFSIGLTFMAVAGATWQWGPTQQPVVLP 125 Query: 130 PGLAGSVELLGRRLPQYDLVLIVIGPAVLGLLWLLLNRTRWGTLIRAATQDREMAGALGI 189 L G + LLG + Y + LI + V L LL+ RTR+G IRA+ ++ A LGI Sbjct: 126 DWLRGQISLLGLEVGAYRVFLIGVVVIVTVALGLLIERTRFGAQIRASVDNQVAAAGLGI 185 Query: 190 HQSWLFTGVFA 200 + S +F+ FA Sbjct: 186 NVSRVFSLTFA 196 >gi|300311946|ref|YP_003776038.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 94.7 bits (234), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 77/229 (33%), Positives = 120/229 (52%), Gaps = 25/229 (10%) Query: 8 VQLINGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGVFIAYTLVSLIGASG---- 63 +Q NGL+ + L+A GL++IFG ++N AHG F +G +I Y L L Sbjct: 1 MQGFNGLSVFCVLLLMALGLAIIFGQMGVINMAHGEFLTIGAYITYLLSHLTTTYAPALQ 60 Query: 64 -VGFWSAVLLSACAVALLGALVEITVLRRIYRAPELFQLLATFALVLVLKDAALYLWGPE 122 F+ A++LS +G L E+ ++ R+Y+ P L LLAT+ L L ++ ++G + Sbjct: 61 PFYFFVAIVLSFAVAYGVGYLTELLLISRLYKRP-LDTLLATWGLSLAMQQTFRSIFGAK 119 Query: 123 DLFGPRAPGLAGSVELLGRRLPQYDLV-----LIVIGPAVL--GLLWLLLNRTRWGTLIR 175 ++ L GS + P D+ L ++G VL G +++LL R+RWG +R Sbjct: 120 EVSATLPDWLMGSFK------PTDDIDIPLNGLFMMGLTVLLTGAIFVLLFRSRWGLQVR 173 Query: 176 AATQDREMAGALGIHQSWLFTGVFALGCFLAGLGGAL------QGPXSG 218 A Q+R M+GA+GI+ + FALGC +AG+ GA GP SG Sbjct: 174 ATMQNRVMSGAVGINTRKVDRTTFALGCGVAGVAGAAFTTIGSTGPTSG 222 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.138 0.457 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 766,367 Number of extensions: 24879 Number of successful extensions: 194 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 17 Length of query: 322 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 237 Effective length of database: 1,217,395 Effective search space: 288522615 Effective search space used: 288522615 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)