BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0005.p (330 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis tra... 327 7e-91 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 126 3e-30 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 125 6e-30 gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis pro... 104 9e-24 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 103 2e-23 >gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis transducer transmembrane protein I [Herbaspirillum seropedicae SmR1] Length = 547 Score = 327 bits (838), Expect = 7e-91, Method: Compositional matrix adjust. Identities = 170/277 (61%), Positives = 215/277 (77%), Gaps = 1/277 (0%) Query: 1 MKNWKIGVRLTIGFGVVLALLVAGATFGLRQVSKLNDHVEFITSVDEGKLLALSKVQFAI 60 MKN KIG RL +GF VL LLVA A FGL+QVS+LND + F+TSVDEGKL ALSKVQFA+ Sbjct: 1 MKNLKIGHRLGLGFAAVLILLVAVAAFGLQQVSRLNDRIVFLTSVDEGKLEALSKVQFAV 60 Query: 61 GLRAVAARNLVLVTDPVQQKGDIELVTKAQKDIDSGMAELTALMKNPADATAQERNMLEQ 120 GLRA+AARNL LV QK D++LV +Q+DID+GMAEL LM++PA ++ QER MLE+ Sbjct: 61 GLRAIAARNLTLVQSAADQKNDLDLVGSSQQDIDAGMAELGRLMQHPA-SSPQEREMLEK 119 Query: 121 LRALEAKYLPIATKIGALATTQQTEAAKASLIAECMPLLKQVIAHISEFNVVMRARTHES 180 LR LEA+YLP+A I LAT+QQTEAA+ASL+ +CMP+L +VIA + +FN ++R + + Sbjct: 120 LRQLEAQYLPVAVNIVKLATSQQTEAARASLVKDCMPILNRVIAQVEQFNKLLRKNSEAN 179 Query: 181 VTTAQEAYEVSKWTMFALSAVSLLLGSLIAWWLTRSISRPLQEAVTIAQNVSTGDLTSTI 240 V A+ AY ++KWTM A S +L LG ++AW LTRSISRPL +AV++AQ+V +GDL S I Sbjct: 180 VEEAEAAYTLAKWTMLAASLAALTLGGMVAWLLTRSISRPLAQAVSVAQSVQSGDLGSRI 239 Query: 241 TVDSKDELGQLKLALKEMNDNLLRIVGDVRHGTDTXA 277 V SKDELGQL ALK MND+L+ IVG+VR GTDT A Sbjct: 240 EVTSKDELGQLMSALKGMNDSLVNIVGEVRQGTDTIA 276 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 126 bits (316), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 92/277 (33%), Positives = 137/277 (49%), Gaps = 9/277 (3%) Query: 1 MKNWKIGVRLTIGFGVVLALLVAGATFGLRQVSKLNDHVEFITSVDEGKLLALSKVQFAI 60 MKN KIGVRL IGF +L L+ G+ ++ + D + +T + AL K + A+ Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRI------ALKKERDAV 54 Query: 61 GLRAVAARNLVLVTDPVQQKGDIELVTKAQKDIDSGMAELTALMKN--PADATAQERNML 118 A N V T V + D QK ID+ A ++ L K A A+E+ + Sbjct: 55 QWHAAIKENGVR-TFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLF 113 Query: 119 EQLRALEAKYLPIATKIGALATTQQTEAAKASLIAECMPLLKQVIAHISEFNVVMRARTH 178 +++ L A Y I + T A+ + ++ + + + Sbjct: 114 DEVGKLRAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVID 173 Query: 179 ESVTTAQEAYEVSKWTMFALSAVSLLLGSLIAWWLTRSISRPLQEAVTIAQNVSTGDLTS 238 ++ Y + + AL A+ ++LG+ +AW LTRSI+RPL +AVTIA+ V+ GDLTS Sbjct: 174 QTAAAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTS 233 Query: 239 TITVDSKDELGQLKLALKEMNDNLLRIVGDVRHGTDT 275 I S+DE G+L ALK MN NLL IVG VR GT+T Sbjct: 234 RIDSSSRDETGKLLAALKTMNGNLLDIVGRVRMGTET 270 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 125 bits (313), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 82/282 (29%), Positives = 142/282 (50%), Gaps = 19/282 (6%) Query: 1 MKNWKIGVRLTIGFGVVLALLVAGATFGLRQVSKLNDHVEFITSVDEGKLLALSKVQFAI 60 N I RL I F +++ ++ GL +++ +N ++ + + K+ ++ + + Sbjct: 3 FNNLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISGKV 62 Query: 61 GLRAVAARNLVLVTDPVQQKGDIELVTKAQKDIDSGMAE-------LTALMKNPADATAQ 113 A++ RN +L+TDP Q + QK I+S AE LT L+ NP + Sbjct: 63 DDVAISLRNQLLMTDPAQ-------IAAEQKTIESLSAENTKLYDELTRLVANP-----E 110 Query: 114 ERNMLEQLRALEAKYLPIATKIGALATTQQTEAAKASLIAECMPLLKQVIAHISEFNVVM 173 + L+++ Y + +I L ++ +AA +++E +PL K + + E + Sbjct: 111 AKAKLDKVTETRKSYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQ 170 Query: 174 RARTHESVTTAQEAYEVSKWTMFALSAVSLLLGSLIAWWLTRSISRPLQEAVTIAQNVST 233 + +SV A+ AYE ++ M ++ + IAW ++RSI+RPL AV +A+ V+ Sbjct: 171 QKLMDQSVDQAEAAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAA 230 Query: 234 GDLTSTITVDSKDELGQLKLALKEMNDNLLRIVGDVRHGTDT 275 GDLT+ I S DE G+L AL MN L IV +VR GTD+ Sbjct: 231 GDLTAVIAAHSNDETGRLLQALAAMNQKLKAIVLEVRRGTDS 272 >gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 571 Score = 104 bits (260), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 82/284 (28%), Positives = 131/284 (46%), Gaps = 15/284 (5%) Query: 3 NWKIGVRLTIGFGVVLALLVAGATFGLRQVSKLNDHV-----EFITSVDEGKLLALSKVQ 57 N KI +L F VVL ++ F +++ +N ++ S+D ALSK+ Sbjct: 6 NLKIAHKLNAAFLVVLVFIIGLGGFSSLELAAVNKSSTDIARNWLPSID-----ALSKMS 60 Query: 58 FAIGLRAVAARNLVLVTDPVQQKGDIELVTKAQKDIDSGMAELTALMKNPADATAQERNM 117 A+ +L D ++K E K + +A L+ P +E+ + Sbjct: 61 LALARSRSFDMQQLLTADKQEEKASRERADKQMATLLKEVARYGKLVSEP-----REKEL 115 Query: 118 LEQLRALEAKYLPIATKIGALATTQQTEAAKASLIAECMPLLKQVIAHISEFNVVMRART 177 ++ A Y ++ AL Q +AA A + + P+ +++ I E + V Sbjct: 116 YPEIERNIALYKEAHERMSALFEQDQKQAAHALMNSTSTPVYRKLQEQIGELSEVNSKGA 175 Query: 178 HESVTTAQEAYEVSKWTMFALSAVSLLLGSLIAWWLTRSISRPLQEAVTIAQNVSTGDLT 237 S A Y + + AL ++L ++WW+ R ++RPL AV IA+ V+ GDLT Sbjct: 176 ALSDADADHIYGQANMAILALVTTCVVLAMALSWWIARLVARPLANAVDIARQVAAGDLT 235 Query: 238 STITVDSKDELGQLKLALKEMNDNLLRIVGDVRHGTDTXARRSA 281 + I +DE GQL AL++MN NLL IV +VR GTDT SA Sbjct: 236 ADIPTAHRDETGQLLEALRQMNGNLLNIVREVRQGTDTITTASA 279 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 103 bits (257), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 84/275 (30%), Positives = 135/275 (49%), Gaps = 5/275 (1%) Query: 1 MKNWKIGVRLTIGFGVVLALLVAGATFGLRQVSKLNDHVEFITSVDEGKLLALSKVQFAI 60 + + KI VRL F ++ ++ A G++ + ++D + I + K+ +++ + Sbjct: 4 ISDMKISVRLGACFAALIVVMCVIAGVGVKNLLMVSDATDEIVNDRYVKVALGTQITDKV 63 Query: 61 GLRAVAARNLVLVTDPVQQKGDIELVTKAQKDIDSGMAELTALMKNPADATAQERNMLEQ 120 A + RN ++ +P +QK ++ + + +AE+ P + +L Sbjct: 64 NTGARSLRNAIMARNPEEQKKYLDRMDTYTSQVSEHLAEMEKRTNTPRGS-----ELLAA 118 Query: 121 LRALEAKYLPIATKIGALATTQQTEAAKASLIAECMPLLKQVIAHISEFNVVMRARTHES 180 L+ A Y K+ L Q+ + A A L + +P+ + A + E + +S Sbjct: 119 LKQARADYSKERIKVVDLIHQQKRDEATAHLFDKTIPVQDKYFAAVKEMVSFQSSLMDKS 178 Query: 181 VTTAQEAYEVSKWTMFALSAVSLLLGSLIAWWLTRSISRPLQEAVTIAQNVSTGDLTSTI 240 A A + M LS V++LL +L A +TRSI+RPL EAV +A V+ GDLT I Sbjct: 179 AERASAASSSAINIMLVLSGVAVLLSALCAMLITRSITRPLNEAVQVATAVAAGDLTVQI 238 Query: 241 TVDSKDELGQLKLALKEMNDNLLRIVGDVRHGTDT 275 SKDE G L ALK MNDNL RIV +VR GTDT Sbjct: 239 EATSKDETGALLGALKSMNDNLHRIVAEVRAGTDT 273 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 874,267 Number of extensions: 31574 Number of successful extensions: 282 Number of sequences better than 1.0e-08: 43 Number of HSP's gapped: 251 Number of HSP's successfully gapped: 43 Length of query: 330 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 244 Effective length of database: 1,212,660 Effective search space: 295889040 Effective search space used: 295889040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)