BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0005.p (335 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311090|ref|YP_003775182.1| allophanate hydrolase subunit 2... 473 e-134 gi|300313401|ref|YP_003777493.1| allophanate hydrolase subunit 2... 174 7e-45 gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum... 122 6e-29 >gi|300311090|ref|YP_003775182.1| allophanate hydrolase subunit 2 [Herbaspirillum seropedicae SmR1] Length = 344 Score = 473 bits (1216), Expect = e-134, Method: Compositional matrix adjust. Identities = 240/344 (69%), Positives = 279/344 (81%), Gaps = 10/344 (2%) Query: 1 MEIIQPGVLTSVQDLGRPGHRRLGINPGGALNPLALSTANLLVGNDAGAAGLEITMGACE 60 ++IIQPG+L SVQD+GR GHRRLGI+PGGALN LAL++ANLLVGN AAGLEITMG E Sbjct: 2 IDIIQPGMLASVQDMGRYGHRRLGIHPGGALNTLALASANLLVGNPVDAAGLEITMGVAE 61 Query: 61 LRFTADTRIALGGDDFDARLDDKPLAPYWSMPVRAGQTLRLAHVGRGGTP------GMRT 114 LRFT TRIALGGDD A LD KP+A WS+PVRAG L+L+ + +P MRT Sbjct: 62 LRFTRATRIALGGDDIGATLDGKPIAACWSIPVRAGSILKLS-LAAADSPEQAPRHSMRT 120 Query: 115 CLAVAGGIDVPVILDSRSTDLKAGFGGHQGRALRKGDRLPLGDAGVK---ASGPAFGVRS 171 LAVAGGIDVP++L SRSTDLKAGFGGHQGRAL++GD+LP+GDA GPAFGVRS Sbjct: 121 YLAVAGGIDVPLVLGSRSTDLKAGFGGHQGRALKRGDKLPIGDAQAAAAAVHGPAFGVRS 180 Query: 172 PQWSGLSAQSNDSSKLTTLRVLPGPEFEQFTVAARNTLCQDKWRITPNSNRMGYRLEGPE 231 P+WSGL+ ++++ S+ TLRVLP PEF+QF+ +AR L D WR+TPNSNRMGYRLEGPE Sbjct: 181 PRWSGLALRADEHSQHVTLRVLPAPEFDQFSKSAREALWSDTWRVTPNSNRMGYRLEGPE 240 Query: 232 LKRKQSRDLLSHGVVPGVIQVPPSGQPIILMGDAQTTGGYPKIGVVIAADMWKLAQAPLN 291 LKRKQ RD+LSHGVVPGVIQVPPSGQPIILMGDAQTTGGYPKIGV+I AD+WKLAQAPLN Sbjct: 241 LKRKQGRDMLSHGVVPGVIQVPPSGQPIILMGDAQTTGGYPKIGVIIGADLWKLAQAPLN 300 Query: 292 GLLQLQLCDLEQALHAWEEQQRYLAHVEQTVAALDWSRATQYRK 335 L+L+ C++ AL A EEQQRYL + TVAALDWSRAT Y+K Sbjct: 301 ATLRLEPCEMADALAAAEEQQRYLDSIRATVAALDWSRATLYKK 344 >gi|300313401|ref|YP_003777493.1| allophanate hydrolase subunit 2 [Herbaspirillum seropedicae SmR1] Length = 325 Score = 174 bits (442), Expect = 7e-45, Method: Compositional matrix adjust. Identities = 121/320 (37%), Positives = 171/320 (53%), Gaps = 27/320 (8%) Query: 1 MEIIQPGVLTSVQDLGRPGHRRLGINPGGALNPLALSTANLLVGNDAGAAGLEITMGACE 60 +++ QPG+ TSVQD GR G+ LGI P GAL+ AL +ANLLVGN A + LE ++ + Sbjct: 3 IKVKQPGLATSVQDGGREGYYHLGIPPSGALDQYALRSANLLVGNHASTSALECSLLGPQ 62 Query: 61 LRFTADTRIALGGDDFDARLD--DKPLAPYWSMPVRAGQTLRLAHVGRGGTPGMRTCLAV 118 L F +D +A+ G R+D ++PL + VR+GQ L + G R+ LA+ Sbjct: 63 LEFRSDALVAVTGARMQPRVDGSERPLDTAFM--VRSGQVLSFDYP----KAGARSYLAI 116 Query: 119 AGGIDVPVILDSRSTDLKAGFGGHQGRALRKGDRLPLGDAGVKASGPAFGVRSPQWSGLS 178 AGGIDVPV+L+SRST GG QGR L K D LPLG +AS A R+ S + Sbjct: 117 AGGIDVPVVLESRSTYALGALGGWQGRKLAKDDELPLG----RASPKAIEGRTLPLSLVP 172 Query: 179 AQSNDSSKLTTLRVLPGPEFEQFTVAARNTLCQDKWRITPNSNRMGYRLEGPELKRKQSR 238 ++ TLRV+PG + T A D W + ++R+GYR +G + + + R Sbjct: 173 VYDREA----TLRVVPGLYIHRLTDEAVERFFDDTWTVGSEADRIGYRFKGGQAMQFRPR 228 Query: 239 D-----------LLSHGVVPGVIQVPPSGQPIILMGDAQTTGGYPKIGVVIAADMWKLAQ 287 + ++ G IQ+P +PI+L+ DA + GGY IG VI+AD+ + Q Sbjct: 229 EQPFGAGSDPSNIVDACYPIGSIQIPGGLEPIVLLRDAVSGGGYATIGTVISADLDLIGQ 288 Query: 288 APLNGLLQLQLCDLEQALHA 307 N Q LEQAL A Sbjct: 289 LQPNHKAQFVRVTLEQALEA 308 >gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum seropedicae SmR1] Length = 1207 Score = 122 bits (305), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 107/302 (35%), Positives = 142/302 (47%), Gaps = 28/302 (9%) Query: 1 MEIIQPGVLTSVQDL-GRPGHRRLGINPGGALNPLALSTANLLVGNDAGAAGLEITMGAC 59 +E++ PG T+VQD GR G+ +G+ P G ++ AL N L+GN AAGLE+TM Sbjct: 455 VEVLAPGTQTTVQDHPGRIGYWAVGVPPSGPMDERALRLGNRLLGNAEEAAGLEVTMSGP 514 Query: 60 ELRFTADTRIALGGDDFDARLD--DKPLAPYWSMPVRAGQTLRLAHVGRGGTPGMRTCLA 117 LRF + + G LD +P+A + + AG TL+L + G R+ LA Sbjct: 515 ILRFNTAAMVVVTGAVIPVLLDGVAQPMATV--LHIAAGSTLKLGAISGAGA---RSYLA 569 Query: 118 VAGGIDVPVILDSRSTDLKAGFGGHQGRALRKGDRLPLGDAGVKASGPAFGVRSPQWSGL 177 V GG+ VP L SRST FGGH GRAL GD L L A A G P Sbjct: 570 VRGGVQVPTYLGSRSTFTLGQFGGHAGRALASGDILHLAPLAADAMTQA-GQTLP----- 623 Query: 178 SAQSNDSSKLTTLRVLPGPEF--EQFTVAARNTLCQDKWRITPNSNRMGYRLEGPELKRK 235 +A + +RV+ GP E FT A T W + NS+R G RL GP K + Sbjct: 624 AALYPALEAVRQVRVIYGPHGAPEYFTPAYMETFFATDWEVHFNSSRTGVRLIGP--KPE 681 Query: 236 QSRDLLSH-GVVPGVIQVPPSG---------QPIILMGDAQTTGGYPKIGVVIAADMWKL 285 RD G+ P I P P+IL D + GG+ VI AD+W++ Sbjct: 682 WVRDSGGEAGLHPSNIHDNPYAVGAVDFTGDMPVILGPDGPSLGGFVCPVTVIEADLWQI 741 Query: 286 AQ 287 Q Sbjct: 742 GQ 743 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,167,846 Number of extensions: 53235 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 3 Length of query: 335 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 249 Effective length of database: 1,212,660 Effective search space: 301952340 Effective search space used: 301952340 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)