BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0022.0016.p
         (177 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312440|ref|YP_003776532.1| threonine efflux protein [Herba...   245   2e-66
gi|300314064|ref|YP_003778156.1| threonine efflux protein [Herba...    67   7e-13
gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herba...    64   5e-12

>gi|300312440|ref|YP_003776532.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 208

 Score =  245 bits (625), Expect = 2e-66,   Method: Compositional matrix adjust.
 Identities = 130/173 (75%), Positives = 145/173 (83%), Gaps = 4/173 (2%)

Query: 1   MPDVFLFLVAAMMLTLAPGPDNIYVLTRGVAQGKKAGFVAALGFSSGLFFHTLLAVLGFA 60
           MPD+ LFL+AAM LTLAPGPDN+YVLTRG+AQG+KAG VAA GF SGL FHTLLAVLGFA
Sbjct: 1   MPDLILFLLAAMTLTLAPGPDNLYVLTRGIAQGRKAGLVAAAGFCSGLVFHTLLAVLGFA 60

Query: 61  AIIKAYPAAYHALQYAGAAYLVYLGIRTLRSAASMSLEAT----MTPVSLSRIYWQSVIA 116
           A+IKAYP AYHALQYAGAAYL YLG+RTLRSA++    A        V L RIYWQSV+A
Sbjct: 61  ALIKAYPPAYHALQYAGAAYLAYLGVRTLRSASAGLALAADGQGAAAVPLRRIYWQSVLA 120

Query: 117 NILNPKVTLFFIAFLPQFVNVQAGHVAWQMLLLAVVFILQALAIFSAIAFFSG 169
           N+LNPKVTLFFIAFLPQFV  +AGH A QML+LA+VFI+QA AIFSAIA FSG
Sbjct: 121 NMLNPKVTLFFIAFLPQFVQREAGHEALQMLVLALVFIVQAFAIFSAIALFSG 173


>gi|300314064|ref|YP_003778156.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 210

 Score = 67.4 bits (163), Expect = 7e-13,   Method: Compositional matrix adjust.
 Identities = 48/136 (35%), Positives = 69/136 (50%), Gaps = 2/136 (1%)

Query: 1   MPDVFLFLVAAMMLTLAPGPDNIYVLTRGVAQGKKAGFVAALGFSSGLFFHTLLAVLGFA 60
           + D++  + A M+  L PGP    VLT     G + GF A  G   G      LA  G A
Sbjct: 10  ITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFLAAAGVA 69

Query: 61  AIIKAYPAAYHALQYAGAAYLVYLGIRTLRSAASMSLEATMTPVSLSRIYWQSVIANILN 120
           A++ A P  +H LQY GAAYL YLG R L +        T+ P S +  + +  +  + N
Sbjct: 70  ALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGG--GTVVPFSNAAEFRRGFLVTLFN 127

Query: 121 PKVTLFFIAFLPQFVN 136
           PK  +F++AF P F++
Sbjct: 128 PKAIVFYMAFFPLFLD 143


>gi|300311201|ref|YP_003775293.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 219

 Score = 64.3 bits (155), Expect = 5e-12,   Method: Compositional matrix adjust.
 Identities = 47/181 (25%), Positives = 82/181 (45%), Gaps = 14/181 (7%)

Query: 5   FLFLVAAMMLTLAPGPDNIYVLTRGVAQGKKAGFVAALGFSSGLFFHTLLAVLGFAAIIK 64
           F F  AA ++ ++PG   +  ++ G+  G +      LG    L    ++A  G  +++ 
Sbjct: 7   FAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAGVGSLLV 66

Query: 65  AYPAAYHALQYAGAAYLVYLGIRTLRS-------AASMSLEATMTPVSLSRIYWQSV--- 114
           A   A++ ++ AGA YL+YLG    R+         ++            + +WQ     
Sbjct: 67  ASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWWQRCMTG 126

Query: 115 -IANILNPKVTLFFIAFLPQFVNVQAGHVAW-QMLLLAVVFILQALAIFSAIAFFSGRPE 172
            + N  NPK  +F +A LPQF++       W Q+L+LAV  +   L +    AF + R +
Sbjct: 127 FLTNATNPKGIVFMVAVLPQFID--HDRPLWLQLLVLAVTMVAVDLVVMHGYAFAASRLQ 184

Query: 173 R 173
           R
Sbjct: 185 R 185


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.331    0.140    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 437,110
Number of extensions: 13604
Number of successful extensions: 81
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 77
Number of HSP's successfully gapped: 4
Length of query: 177
Length of database: 1,619,870
Length adjustment: 79
Effective length of query: 98
Effective length of database: 1,245,805
Effective search space: 122088890
Effective search space used: 122088890
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 127 (53.5 bits)